Endogenous nitric oxide inhibits neutrophil adherence to lung epithelial cells to modulate interleukin-8 release

Horng Chyuan Lin, Chun Hua Wang, Chih Teng Yu, Kuo Shiung Hwang, Han Pin Kuo

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To investigate the effect of neutrophil adherence to epithelial cells on the release of interleukin 8 (IL-8), we measured neutrophil adherence in the presence or absence of IFN-γ+TNF-α+IL-1β (cytomix) stimulation on cultured A549 epithelial cells. The extent of neutrophil adherence to A549 epithelial cells was measured and the concomitant production of IL-8 and nitrite were assayed. The roles of adhesion molecules and nitrite in modulation of neutrophil adherence were examined by pretreatment with oversaturating ICAM-1 blocking antibody and L-NAME (1mM), respectively. There was a time-dependent spontaneous and cytomix-induced release of IL-8 from epithelial cells, as well as a time-dependent increase in the magnitude of neutrophil adherence to epithelial cells. Stimulation of epithelial cells with cytomix induced a further increase in neutrophil adherence. Pretreatment with oversaturated ICAM-1 monoclonal antibody inhibited neutrophil adherence with or without cytomix stimulation. The inhibition of neutrophil adherence to epithelial cells with ICAM-1 monoclonal antibody or a semipermeable membrane downregulated the release of IL-8 with or without cytomix stimulation. Stimulation with cytomix decreased nitrite production. Both neutrophil adherence and L-NAME pretreatment significantly inhibited the production of nitrite. The inhibition of neutrophil adherence to epithelial cells with ICAM-1 monoclonal antibody or a semipermeable membrane upregulated nitrite production. Pretreatment with L-NAME failed to modify the spontaneous release of IL-8, but significantly enhanced the response to adherence and cytomix. In conclusion, endogenous nitric oxide may play a role in preventing neutrophil adherence to lung epithelial cells, thus modulating concomitant IL-8 release.

Original languageEnglish
Pages (from-to)1333-1344
Number of pages12
JournalLife Sciences
Volume69
Issue number11
DOIs
Publication statusPublished - Aug 3 2001
Externally publishedYes

Fingerprint

Interleukin-8
Nitric Oxide
Neutrophils
Epithelial Cells
Lung
Nitrites
Intercellular Adhesion Molecule-1
NG-Nitroarginine Methyl Ester
Monoclonal Antibodies
Membranes
Blocking Antibodies
Interleukin-1
Adhesion
Modulation
Cultured Cells
Down-Regulation
Molecules

Keywords

  • Adhesion molecules
  • Epithelial cell
  • IL-8
  • Neutrophil
  • Nitric oxide

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Endogenous nitric oxide inhibits neutrophil adherence to lung epithelial cells to modulate interleukin-8 release. / Lin, Horng Chyuan; Wang, Chun Hua; Yu, Chih Teng; Hwang, Kuo Shiung; Kuo, Han Pin.

In: Life Sciences, Vol. 69, No. 11, 03.08.2001, p. 1333-1344.

Research output: Contribution to journalArticle

Lin, Horng Chyuan ; Wang, Chun Hua ; Yu, Chih Teng ; Hwang, Kuo Shiung ; Kuo, Han Pin. / Endogenous nitric oxide inhibits neutrophil adherence to lung epithelial cells to modulate interleukin-8 release. In: Life Sciences. 2001 ; Vol. 69, No. 11. pp. 1333-1344.
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