Endobronchial ultrasound increases the diagnostic yields of polymerase chain reaction and smear for pulmonary tuberculosis

Shu Min Lin, Yung Lun Ni, Chih Hsia Kuo, Ting Yu Lin, Tsai Yu Wang, Fu Tsai Chung, Han Pin Kuo

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objectives: Our objective was to determine the contribution of endobronchial ultrasound in the diagnostic yields of acid-fast bacillus smear, nucleic acid amplification tests, and culture in bronchoalveolar lavage fluid for pulmonary tuberculosis. Methods: During a 1-year interval, 99 patients who had initial sputum-negative acid-fast bacillus smears or no sputum but were later proven to have a positive culture for Mycobacterium tuberculosis in their sputum or bronchoalveolar lavage fluid were retrospectively studied. Among them, 56 patients underwent bronchoscopy with endobronchial ultrasound (EBUS group) and 43 patients received conventional bronchoscopy for bronchoalveolar lavage (non-EBUS group). Results: The diagnostic yields of the nucleic acid amplification tests (89.3%, 50/56; P = .006), acid-fast bacillus smear (30.4%, 17/56; P = .013), and M tuberculosis culture in bronchoalveolar lavage fluid (67.9%, 38/56; P = .041) were significantly higher in the EBUS group of patients. The results of those who underwent conventional bronchoscopy were 65.1% (28/43), 9.3% (4/43), and 46.5% (20/43), respectively. Combining bronchoalveolar lavage fluid smear and nucleic acid amplification tests, we made a rapid diagnosis of pulmonary tuberculosis in 51 (91.1%) of the 56 EBUS patients and 29 (67.4%; P = .004) of the 43 non-EBUS patients. Conclusions: The introduction of endobronchial ultrasound increases the diagnostic yield of the nucleic acid amplification tests, acid-fast bacillus smear, and M tuberculosis culture from bronchioalveolar lavage fluid in patients with pulmonary tuberculosis who have negative sputum smear or no sputum production.

Original languageEnglish
Pages (from-to)1554-1560
Number of pages7
JournalJournal of Thoracic and Cardiovascular Surgery
Volume139
Issue number6
DOIs
Publication statusPublished - Jun 1 2010
Externally publishedYes

Fingerprint

Pulmonary Tuberculosis
Ultrasonography
Nucleic Acid Amplification Techniques
Sputum
Polymerase Chain Reaction
Bronchoalveolar Lavage Fluid
Bacillus
Bronchoscopy
Acids
Bronchoalveolar Lavage
Tuberculosis
Mycobacterium tuberculosis

ASJC Scopus subject areas

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

Endobronchial ultrasound increases the diagnostic yields of polymerase chain reaction and smear for pulmonary tuberculosis. / Lin, Shu Min; Ni, Yung Lun; Kuo, Chih Hsia; Lin, Ting Yu; Wang, Tsai Yu; Chung, Fu Tsai; Kuo, Han Pin.

In: Journal of Thoracic and Cardiovascular Surgery, Vol. 139, No. 6, 01.06.2010, p. 1554-1560.

Research output: Contribution to journalArticle

Lin, Shu Min ; Ni, Yung Lun ; Kuo, Chih Hsia ; Lin, Ting Yu ; Wang, Tsai Yu ; Chung, Fu Tsai ; Kuo, Han Pin. / Endobronchial ultrasound increases the diagnostic yields of polymerase chain reaction and smear for pulmonary tuberculosis. In: Journal of Thoracic and Cardiovascular Surgery. 2010 ; Vol. 139, No. 6. pp. 1554-1560.
@article{bf85ce7837124a60bc81edc7b68c81b7,
title = "Endobronchial ultrasound increases the diagnostic yields of polymerase chain reaction and smear for pulmonary tuberculosis",
abstract = "Objectives: Our objective was to determine the contribution of endobronchial ultrasound in the diagnostic yields of acid-fast bacillus smear, nucleic acid amplification tests, and culture in bronchoalveolar lavage fluid for pulmonary tuberculosis. Methods: During a 1-year interval, 99 patients who had initial sputum-negative acid-fast bacillus smears or no sputum but were later proven to have a positive culture for Mycobacterium tuberculosis in their sputum or bronchoalveolar lavage fluid were retrospectively studied. Among them, 56 patients underwent bronchoscopy with endobronchial ultrasound (EBUS group) and 43 patients received conventional bronchoscopy for bronchoalveolar lavage (non-EBUS group). Results: The diagnostic yields of the nucleic acid amplification tests (89.3{\%}, 50/56; P = .006), acid-fast bacillus smear (30.4{\%}, 17/56; P = .013), and M tuberculosis culture in bronchoalveolar lavage fluid (67.9{\%}, 38/56; P = .041) were significantly higher in the EBUS group of patients. The results of those who underwent conventional bronchoscopy were 65.1{\%} (28/43), 9.3{\%} (4/43), and 46.5{\%} (20/43), respectively. Combining bronchoalveolar lavage fluid smear and nucleic acid amplification tests, we made a rapid diagnosis of pulmonary tuberculosis in 51 (91.1{\%}) of the 56 EBUS patients and 29 (67.4{\%}; P = .004) of the 43 non-EBUS patients. Conclusions: The introduction of endobronchial ultrasound increases the diagnostic yield of the nucleic acid amplification tests, acid-fast bacillus smear, and M tuberculosis culture from bronchioalveolar lavage fluid in patients with pulmonary tuberculosis who have negative sputum smear or no sputum production.",
author = "Lin, {Shu Min} and Ni, {Yung Lun} and Kuo, {Chih Hsia} and Lin, {Ting Yu} and Wang, {Tsai Yu} and Chung, {Fu Tsai} and Kuo, {Han Pin}",
year = "2010",
month = "6",
day = "1",
doi = "10.1016/j.jtcvs.2010.02.019",
language = "English",
volume = "139",
pages = "1554--1560",
journal = "Journal of Thoracic and Cardiovascular Surgery",
issn = "0022-5223",
publisher = "Mosby Inc.",
number = "6",

}

TY - JOUR

T1 - Endobronchial ultrasound increases the diagnostic yields of polymerase chain reaction and smear for pulmonary tuberculosis

AU - Lin, Shu Min

AU - Ni, Yung Lun

AU - Kuo, Chih Hsia

AU - Lin, Ting Yu

AU - Wang, Tsai Yu

AU - Chung, Fu Tsai

AU - Kuo, Han Pin

PY - 2010/6/1

Y1 - 2010/6/1

N2 - Objectives: Our objective was to determine the contribution of endobronchial ultrasound in the diagnostic yields of acid-fast bacillus smear, nucleic acid amplification tests, and culture in bronchoalveolar lavage fluid for pulmonary tuberculosis. Methods: During a 1-year interval, 99 patients who had initial sputum-negative acid-fast bacillus smears or no sputum but were later proven to have a positive culture for Mycobacterium tuberculosis in their sputum or bronchoalveolar lavage fluid were retrospectively studied. Among them, 56 patients underwent bronchoscopy with endobronchial ultrasound (EBUS group) and 43 patients received conventional bronchoscopy for bronchoalveolar lavage (non-EBUS group). Results: The diagnostic yields of the nucleic acid amplification tests (89.3%, 50/56; P = .006), acid-fast bacillus smear (30.4%, 17/56; P = .013), and M tuberculosis culture in bronchoalveolar lavage fluid (67.9%, 38/56; P = .041) were significantly higher in the EBUS group of patients. The results of those who underwent conventional bronchoscopy were 65.1% (28/43), 9.3% (4/43), and 46.5% (20/43), respectively. Combining bronchoalveolar lavage fluid smear and nucleic acid amplification tests, we made a rapid diagnosis of pulmonary tuberculosis in 51 (91.1%) of the 56 EBUS patients and 29 (67.4%; P = .004) of the 43 non-EBUS patients. Conclusions: The introduction of endobronchial ultrasound increases the diagnostic yield of the nucleic acid amplification tests, acid-fast bacillus smear, and M tuberculosis culture from bronchioalveolar lavage fluid in patients with pulmonary tuberculosis who have negative sputum smear or no sputum production.

AB - Objectives: Our objective was to determine the contribution of endobronchial ultrasound in the diagnostic yields of acid-fast bacillus smear, nucleic acid amplification tests, and culture in bronchoalveolar lavage fluid for pulmonary tuberculosis. Methods: During a 1-year interval, 99 patients who had initial sputum-negative acid-fast bacillus smears or no sputum but were later proven to have a positive culture for Mycobacterium tuberculosis in their sputum or bronchoalveolar lavage fluid were retrospectively studied. Among them, 56 patients underwent bronchoscopy with endobronchial ultrasound (EBUS group) and 43 patients received conventional bronchoscopy for bronchoalveolar lavage (non-EBUS group). Results: The diagnostic yields of the nucleic acid amplification tests (89.3%, 50/56; P = .006), acid-fast bacillus smear (30.4%, 17/56; P = .013), and M tuberculosis culture in bronchoalveolar lavage fluid (67.9%, 38/56; P = .041) were significantly higher in the EBUS group of patients. The results of those who underwent conventional bronchoscopy were 65.1% (28/43), 9.3% (4/43), and 46.5% (20/43), respectively. Combining bronchoalveolar lavage fluid smear and nucleic acid amplification tests, we made a rapid diagnosis of pulmonary tuberculosis in 51 (91.1%) of the 56 EBUS patients and 29 (67.4%; P = .004) of the 43 non-EBUS patients. Conclusions: The introduction of endobronchial ultrasound increases the diagnostic yield of the nucleic acid amplification tests, acid-fast bacillus smear, and M tuberculosis culture from bronchioalveolar lavage fluid in patients with pulmonary tuberculosis who have negative sputum smear or no sputum production.

UR - http://www.scopus.com/inward/record.url?scp=77952300958&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952300958&partnerID=8YFLogxK

U2 - 10.1016/j.jtcvs.2010.02.019

DO - 10.1016/j.jtcvs.2010.02.019

M3 - Article

C2 - 20494195

AN - SCOPUS:77952300958

VL - 139

SP - 1554

EP - 1560

JO - Journal of Thoracic and Cardiovascular Surgery

JF - Journal of Thoracic and Cardiovascular Surgery

SN - 0022-5223

IS - 6

ER -