Encephalitis induced by a newly discovered ruminant rhadinovirus in a free-living formosan sambar deer (Rusa unicolor swinhoei)

A.-M. Chang, C.-C. Chen, C.-D. Chang, Y.-L. Huang, G.-M. Ke, B.A. Walther

Research output: Contribution to journalArticle

Abstract

We documented a case of a free-living Formosan sambar deer (Rusa unicolor swinhoei) infected with a newly discovered ruminant Rhadinovirus (RuRv). Non-purulent encephalitis was the primary histological lesion of the sambar deer. We conducted nested PCR to screen for herpesvirus using generic primers targeting the DNA polymerase gene. In addition, we found that DNA polymerase gene of the sambar deer RuRv was present in the macrophage distributed in the Virchow Robin space with histopathologic lesions by chromogenic in-situ hybridization (CISH). The phylogenetic analysis indicated a high similarity between the viral sequence isolated from fallow deer and our case. This result suggests the possibility of cross-species transmission from other exotic Cervidae reservoir to the Formosan sambar deer. © 2018 The Japanese Society of Veterinary Science.
Original languageEnglish
Pages (from-to)810-813
Number of pages4
JournalJournal of Veterinary Medical Science
Volume80
Issue number5
DOIs
Publication statusPublished - 2018

Fingerprint

Rhadinovirus
Deer
Ruminants
Encephalitis
encephalitis
ruminants
DNA-directed DNA polymerase
DNA-Directed DNA Polymerase
Cervidae
Songbirds
Cervus dama
Herpesviridae
in situ hybridization
veterinary medicine
Genes
In Situ Hybridization
macrophages
genes
Macrophages
Rusa unicolor

Keywords

  • Cervidae
  • Formosan sambar deer
  • Ruminant rhadinovirus
  • animal
  • case report
  • cell line
  • classification
  • deer
  • herpes virus infection
  • isolation and purification
  • male
  • molecular typing
  • phylogeny
  • real time polymerase chain reaction
  • Rhadinovirus
  • veterinary medicine
  • virology
  • virus encephalitis
  • Animals
  • Cell Line
  • Deer
  • Encephalitis, Viral
  • Herpesviridae Infections
  • Male
  • Molecular Typing
  • Phylogeny
  • Real-Time Polymerase Chain Reaction

Cite this

Encephalitis induced by a newly discovered ruminant rhadinovirus in a free-living formosan sambar deer (Rusa unicolor swinhoei). / Chang, A.-M.; Chen, C.-C.; Chang, C.-D.; Huang, Y.-L.; Ke, G.-M.; Walther, B.A.

In: Journal of Veterinary Medical Science, Vol. 80, No. 5, 2018, p. 810-813.

Research output: Contribution to journalArticle

Chang, A.-M. ; Chen, C.-C. ; Chang, C.-D. ; Huang, Y.-L. ; Ke, G.-M. ; Walther, B.A. / Encephalitis induced by a newly discovered ruminant rhadinovirus in a free-living formosan sambar deer (Rusa unicolor swinhoei). In: Journal of Veterinary Medical Science. 2018 ; Vol. 80, No. 5. pp. 810-813.
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abstract = "We documented a case of a free-living Formosan sambar deer (Rusa unicolor swinhoei) infected with a newly discovered ruminant Rhadinovirus (RuRv). Non-purulent encephalitis was the primary histological lesion of the sambar deer. We conducted nested PCR to screen for herpesvirus using generic primers targeting the DNA polymerase gene. In addition, we found that DNA polymerase gene of the sambar deer RuRv was present in the macrophage distributed in the Virchow Robin space with histopathologic lesions by chromogenic in-situ hybridization (CISH). The phylogenetic analysis indicated a high similarity between the viral sequence isolated from fallow deer and our case. This result suggests the possibility of cross-species transmission from other exotic Cervidae reservoir to the Formosan sambar deer. {\circledC} 2018 The Japanese Society of Veterinary Science.",
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author = "A.-M. Chang and C.-C. Chen and C.-D. Chang and Y.-L. Huang and G.-M. Ke and B.A. Walther",
note = "Export Date: 12 October 2018 通訊地址: Chen, C.-C.; Institute of wildlife conservation, College of Veterinary Medicine, National Pingtung University of Science and Technology, 1, Shuefu Road, Taiwan; 電子郵件: ccchen@mail.npust.edu.tw 出資正文: ACKNOWLEDGMENTS. This study was supported by Yushan National Park, Construction and Planning Administration, Ministry 參考文獻: Baxter, S.I.F., Pow, I., Bridgen, A., Reid, H.W., PCR detection of the sheep-associated agent of malignant catarrhal fever (1993) Arch. Virol., 132, pp. 145-159; Ensser, A., Neipel, F., Fickenscher, H., Rhadinovirus pathogenesis (2002) Structure-Function Relationships of Human Pathogenic Viruses, pp. 349-429. , Holzenburg, A. and Bogner, EKluwer Academic Publishers, New York; (2014) Schedule of Protected Wildlife, , http://www.conservation.forest.gov.tw, Forest Bureau, Council of Agriculture, Executive Yuan, Taipei, Taiwan, accessed on August, 20; Li, H., Gailbreath, K., Flach, E.J., Taus, N.S., Cooley, J., Keller, J., Russell, G.C., Crawford, T.B., A novel subgroup of rhadinoviruses in ruminants (2005) J. Gen. Virol., 86, pp. 3021-3026; McGeoch, D.J., Gatherer, D., Dolan, A., On phylogenetic relationships among major lineages of the Gammaherpesvirinae (2005) J. Gen. Virol., 86, pp. 307-316; Nei, M., Kumar, S., (2000) Molecular Evolution and Phylogenetics, , Oxford University Press, New York; O’Toole, D., Li, H., The pathology of malignant catarrhal fever, with an emphasis on ovine herpesvirus 2 (2014) Vet. Pathol., 51, pp. 437-452; Palacios, G., Cowled, C., Bussetti, A.V., Savji, N., Weir, R., Wick, I., Travassos da Rosa, A., Lipkin, W.I., Rapid molecular strategy for orbivirus detection and characterization (2011) J. Clin. Microbiol., 49, pp. 2314-2317; Pitra, C., Fickel, J., Meijaard, E., Groves, P.C., Evolution and phylogeny of old world deer (2004) Mol. Phylogenet. Evol., 33, pp. 880-895; Plowright, W., Ferris, R.D., Scott, G.R., Blue wildebeest and the aetiological agent of bovine malignant catarrhal fever (1960) Nature, 188, pp. 1167-1169; Russell, G.C., Stewart, J.P., Haig, D.M., Malignant catarrhal fever: A review (2009) Vet. J., 179, pp. 324-335; Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S., MEGA6: Molecular evolutionary genetics analysis version 6.0 (2013) Mol. Biol. Evol., 30, pp. 2725-2729; Vandevanter, D.R., Warrener, P., Bennett, L., Schultz, E.R., Coulter, S., Garber, R.L., Rose, T.M., Detection and analysis of diverse herpesviral species by consensus primer PCR (1996) J. Clin. Microbiol., 34, pp. 1666-1671; Yen, S.C., Wang, Y., Ou, H.Y., Habitat of the vulnerable Formosan sambar deer Rusa unicolor swinhoii in Taiwan (2014) Oryx, 48, pp. 232-240",
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TY - JOUR

T1 - Encephalitis induced by a newly discovered ruminant rhadinovirus in a free-living formosan sambar deer (Rusa unicolor swinhoei)

AU - Chang, A.-M.

AU - Chen, C.-C.

AU - Chang, C.-D.

AU - Huang, Y.-L.

AU - Ke, G.-M.

AU - Walther, B.A.

N1 - Export Date: 12 October 2018 通訊地址: Chen, C.-C.; Institute of wildlife conservation, College of Veterinary Medicine, National Pingtung University of Science and Technology, 1, Shuefu Road, Taiwan; 電子郵件: ccchen@mail.npust.edu.tw 出資正文: ACKNOWLEDGMENTS. This study was supported by Yushan National Park, Construction and Planning Administration, Ministry 參考文獻: Baxter, S.I.F., Pow, I., Bridgen, A., Reid, H.W., PCR detection of the sheep-associated agent of malignant catarrhal fever (1993) Arch. Virol., 132, pp. 145-159; Ensser, A., Neipel, F., Fickenscher, H., Rhadinovirus pathogenesis (2002) Structure-Function Relationships of Human Pathogenic Viruses, pp. 349-429. , Holzenburg, A. and Bogner, EKluwer Academic Publishers, New York; (2014) Schedule of Protected Wildlife, , http://www.conservation.forest.gov.tw, Forest Bureau, Council of Agriculture, Executive Yuan, Taipei, Taiwan, accessed on August, 20; Li, H., Gailbreath, K., Flach, E.J., Taus, N.S., Cooley, J., Keller, J., Russell, G.C., Crawford, T.B., A novel subgroup of rhadinoviruses in ruminants (2005) J. Gen. Virol., 86, pp. 3021-3026; McGeoch, D.J., Gatherer, D., Dolan, A., On phylogenetic relationships among major lineages of the Gammaherpesvirinae (2005) J. Gen. Virol., 86, pp. 307-316; Nei, M., Kumar, S., (2000) Molecular Evolution and Phylogenetics, , Oxford University Press, New York; O’Toole, D., Li, H., The pathology of malignant catarrhal fever, with an emphasis on ovine herpesvirus 2 (2014) Vet. Pathol., 51, pp. 437-452; Palacios, G., Cowled, C., Bussetti, A.V., Savji, N., Weir, R., Wick, I., Travassos da Rosa, A., Lipkin, W.I., Rapid molecular strategy for orbivirus detection and characterization (2011) J. Clin. Microbiol., 49, pp. 2314-2317; Pitra, C., Fickel, J., Meijaard, E., Groves, P.C., Evolution and phylogeny of old world deer (2004) Mol. Phylogenet. Evol., 33, pp. 880-895; Plowright, W., Ferris, R.D., Scott, G.R., Blue wildebeest and the aetiological agent of bovine malignant catarrhal fever (1960) Nature, 188, pp. 1167-1169; Russell, G.C., Stewart, J.P., Haig, D.M., Malignant catarrhal fever: A review (2009) Vet. J., 179, pp. 324-335; Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S., MEGA6: Molecular evolutionary genetics analysis version 6.0 (2013) Mol. Biol. Evol., 30, pp. 2725-2729; Vandevanter, D.R., Warrener, P., Bennett, L., Schultz, E.R., Coulter, S., Garber, R.L., Rose, T.M., Detection and analysis of diverse herpesviral species by consensus primer PCR (1996) J. Clin. Microbiol., 34, pp. 1666-1671; Yen, S.C., Wang, Y., Ou, H.Y., Habitat of the vulnerable Formosan sambar deer Rusa unicolor swinhoii in Taiwan (2014) Oryx, 48, pp. 232-240

PY - 2018

Y1 - 2018

N2 - We documented a case of a free-living Formosan sambar deer (Rusa unicolor swinhoei) infected with a newly discovered ruminant Rhadinovirus (RuRv). Non-purulent encephalitis was the primary histological lesion of the sambar deer. We conducted nested PCR to screen for herpesvirus using generic primers targeting the DNA polymerase gene. In addition, we found that DNA polymerase gene of the sambar deer RuRv was present in the macrophage distributed in the Virchow Robin space with histopathologic lesions by chromogenic in-situ hybridization (CISH). The phylogenetic analysis indicated a high similarity between the viral sequence isolated from fallow deer and our case. This result suggests the possibility of cross-species transmission from other exotic Cervidae reservoir to the Formosan sambar deer. © 2018 The Japanese Society of Veterinary Science.

AB - We documented a case of a free-living Formosan sambar deer (Rusa unicolor swinhoei) infected with a newly discovered ruminant Rhadinovirus (RuRv). Non-purulent encephalitis was the primary histological lesion of the sambar deer. We conducted nested PCR to screen for herpesvirus using generic primers targeting the DNA polymerase gene. In addition, we found that DNA polymerase gene of the sambar deer RuRv was present in the macrophage distributed in the Virchow Robin space with histopathologic lesions by chromogenic in-situ hybridization (CISH). The phylogenetic analysis indicated a high similarity between the viral sequence isolated from fallow deer and our case. This result suggests the possibility of cross-species transmission from other exotic Cervidae reservoir to the Formosan sambar deer. © 2018 The Japanese Society of Veterinary Science.

KW - Cervidae

KW - Formosan sambar deer

KW - Ruminant rhadinovirus

KW - animal

KW - case report

KW - cell line

KW - classification

KW - deer

KW - herpes virus infection

KW - isolation and purification

KW - male

KW - molecular typing

KW - phylogeny

KW - real time polymerase chain reaction

KW - Rhadinovirus

KW - veterinary medicine

KW - virology

KW - virus encephalitis

KW - Animals

KW - Cell Line

KW - Deer

KW - Encephalitis, Viral

KW - Herpesviridae Infections

KW - Male

KW - Molecular Typing

KW - Phylogeny

KW - Real-Time Polymerase Chain Reaction

KW - Cervidae

KW - Formosan sambar deer

KW - Ruminant rhadinovirus

U2 - 10.1292/jvms.17-0477

DO - 10.1292/jvms.17-0477

M3 - Article

VL - 80

SP - 810

EP - 813

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

SN - 0916-7250

IS - 5

ER -