Encapsula of periosteal stem cells in injectable photopolymerized hydrogel enhances tendon graft osteointegration

Hsia Wei Liu, Chih Hwa Chen, Ching Lin Tsai, Chung Ming Yu, I. Hsuan Lin, Ging Ho Hsiue

Research output: Contribution to journalArticle

Abstract

Photopolymerized hydrogel based on poly(ethylene glycol) diacrylate (PEGDA) was applied to periosteum-derived periosteal stem cell (PSC) encapsulation and orthopedic tissue engineering. To provide support for cell adhesion, hyaluronic acid (HA) was incorporated into the PEGDA solution prior to photopolymerization. HA can be utilized to mimic the extracellular matrix composition as well as control cell growth and differentiation. In vitro studies have demonstrated its ability to encapsulate PSCs to form bone-like tissue in a photopolymerized hydrogel. Osteointegration of a tendon graft within the bone tunnel is a primary concern when employing tendon grafts for ligament reconstruction. This study presents a novel technique for fabricating injectable hydrogel-photoencapsulated PSCs in a bone tunnel to enhance tendon-bone healing. A total of 21 adult New Zealand white rabbits were used. The long extensor digitorum tendon was transplanted into a bone tunnel of the proximal tibia. The tendon was pulled through a drill hole in the proximal tibia and attached to the medial aspect of the tibia. Hydrogel suspension containing PSCs at a concentration of 20 million/mL was injected into the bone tunnel. Histological examination of the tendon-bone interface and biomechanical testing for maximal pull-out load were evaluated at postoperative weeks 3 and 6. Histological analysis of the tendon-bone interface showed an interface fibrous layer formed by the photoencapsulation of PSCs between the tendon and the bone. This layer became progressive integrated with the tendon-bone surface during the healing process. Biomechanical testing revealed higher maximal pull-out strength in experimental groups with a statistically significant difference at 3 and 6 weeks. These results suggest that photopolymerizable PEGDA and HA hydrogels are promising for tissue-engineered stem cell therapy to enhance tendon-bone healing.

Original languageEnglish
Pages (from-to)109-120
Number of pages12
JournalJournal of Musculoskeletal Research
Volume10
Issue number3
DOIs
Publication statusPublished - Sep 2006
Externally publishedYes

Fingerprint

Hydrogel
Tendons
Stem Cells
Transplants
Bone and Bones
Injections
Hyaluronic Acid
Tibia
Periosteum
Hydrogels
Tissue Engineering
Cell- and Tissue-Based Therapy
Ligaments
Cell Adhesion
Orthopedics
Extracellular Matrix
Cell Differentiation
Suspensions
Rabbits

Keywords

  • Hyaluronic acid
  • Hydrogel
  • Periosteal stem cells
  • Photoencapsulation
  • Tendon-bone healing

ASJC Scopus subject areas

  • Surgery

Cite this

Encapsula of periosteal stem cells in injectable photopolymerized hydrogel enhances tendon graft osteointegration. / Liu, Hsia Wei; Chen, Chih Hwa; Tsai, Ching Lin; Yu, Chung Ming; Lin, I. Hsuan; Hsiue, Ging Ho.

In: Journal of Musculoskeletal Research, Vol. 10, No. 3, 09.2006, p. 109-120.

Research output: Contribution to journalArticle

Liu, Hsia Wei ; Chen, Chih Hwa ; Tsai, Ching Lin ; Yu, Chung Ming ; Lin, I. Hsuan ; Hsiue, Ging Ho. / Encapsula of periosteal stem cells in injectable photopolymerized hydrogel enhances tendon graft osteointegration. In: Journal of Musculoskeletal Research. 2006 ; Vol. 10, No. 3. pp. 109-120.
@article{f1010b7fab974181903e00f4bb4357fa,
title = "Encapsula of periosteal stem cells in injectable photopolymerized hydrogel enhances tendon graft osteointegration",
abstract = "Photopolymerized hydrogel based on poly(ethylene glycol) diacrylate (PEGDA) was applied to periosteum-derived periosteal stem cell (PSC) encapsulation and orthopedic tissue engineering. To provide support for cell adhesion, hyaluronic acid (HA) was incorporated into the PEGDA solution prior to photopolymerization. HA can be utilized to mimic the extracellular matrix composition as well as control cell growth and differentiation. In vitro studies have demonstrated its ability to encapsulate PSCs to form bone-like tissue in a photopolymerized hydrogel. Osteointegration of a tendon graft within the bone tunnel is a primary concern when employing tendon grafts for ligament reconstruction. This study presents a novel technique for fabricating injectable hydrogel-photoencapsulated PSCs in a bone tunnel to enhance tendon-bone healing. A total of 21 adult New Zealand white rabbits were used. The long extensor digitorum tendon was transplanted into a bone tunnel of the proximal tibia. The tendon was pulled through a drill hole in the proximal tibia and attached to the medial aspect of the tibia. Hydrogel suspension containing PSCs at a concentration of 20 million/mL was injected into the bone tunnel. Histological examination of the tendon-bone interface and biomechanical testing for maximal pull-out load were evaluated at postoperative weeks 3 and 6. Histological analysis of the tendon-bone interface showed an interface fibrous layer formed by the photoencapsulation of PSCs between the tendon and the bone. This layer became progressive integrated with the tendon-bone surface during the healing process. Biomechanical testing revealed higher maximal pull-out strength in experimental groups with a statistically significant difference at 3 and 6 weeks. These results suggest that photopolymerizable PEGDA and HA hydrogels are promising for tissue-engineered stem cell therapy to enhance tendon-bone healing.",
keywords = "Hyaluronic acid, Hydrogel, Periosteal stem cells, Photoencapsulation, Tendon-bone healing",
author = "Liu, {Hsia Wei} and Chen, {Chih Hwa} and Tsai, {Ching Lin} and Yu, {Chung Ming} and Lin, {I. Hsuan} and Hsiue, {Ging Ho}",
year = "2006",
month = "9",
doi = "10.1142/S0218957706001820",
language = "English",
volume = "10",
pages = "109--120",
journal = "Journal of Musculoskeletal Research",
issn = "0218-9577",
publisher = "World Scientific Publishing Co. Pte Ltd",
number = "3",

}

TY - JOUR

T1 - Encapsula of periosteal stem cells in injectable photopolymerized hydrogel enhances tendon graft osteointegration

AU - Liu, Hsia Wei

AU - Chen, Chih Hwa

AU - Tsai, Ching Lin

AU - Yu, Chung Ming

AU - Lin, I. Hsuan

AU - Hsiue, Ging Ho

PY - 2006/9

Y1 - 2006/9

N2 - Photopolymerized hydrogel based on poly(ethylene glycol) diacrylate (PEGDA) was applied to periosteum-derived periosteal stem cell (PSC) encapsulation and orthopedic tissue engineering. To provide support for cell adhesion, hyaluronic acid (HA) was incorporated into the PEGDA solution prior to photopolymerization. HA can be utilized to mimic the extracellular matrix composition as well as control cell growth and differentiation. In vitro studies have demonstrated its ability to encapsulate PSCs to form bone-like tissue in a photopolymerized hydrogel. Osteointegration of a tendon graft within the bone tunnel is a primary concern when employing tendon grafts for ligament reconstruction. This study presents a novel technique for fabricating injectable hydrogel-photoencapsulated PSCs in a bone tunnel to enhance tendon-bone healing. A total of 21 adult New Zealand white rabbits were used. The long extensor digitorum tendon was transplanted into a bone tunnel of the proximal tibia. The tendon was pulled through a drill hole in the proximal tibia and attached to the medial aspect of the tibia. Hydrogel suspension containing PSCs at a concentration of 20 million/mL was injected into the bone tunnel. Histological examination of the tendon-bone interface and biomechanical testing for maximal pull-out load were evaluated at postoperative weeks 3 and 6. Histological analysis of the tendon-bone interface showed an interface fibrous layer formed by the photoencapsulation of PSCs between the tendon and the bone. This layer became progressive integrated with the tendon-bone surface during the healing process. Biomechanical testing revealed higher maximal pull-out strength in experimental groups with a statistically significant difference at 3 and 6 weeks. These results suggest that photopolymerizable PEGDA and HA hydrogels are promising for tissue-engineered stem cell therapy to enhance tendon-bone healing.

AB - Photopolymerized hydrogel based on poly(ethylene glycol) diacrylate (PEGDA) was applied to periosteum-derived periosteal stem cell (PSC) encapsulation and orthopedic tissue engineering. To provide support for cell adhesion, hyaluronic acid (HA) was incorporated into the PEGDA solution prior to photopolymerization. HA can be utilized to mimic the extracellular matrix composition as well as control cell growth and differentiation. In vitro studies have demonstrated its ability to encapsulate PSCs to form bone-like tissue in a photopolymerized hydrogel. Osteointegration of a tendon graft within the bone tunnel is a primary concern when employing tendon grafts for ligament reconstruction. This study presents a novel technique for fabricating injectable hydrogel-photoencapsulated PSCs in a bone tunnel to enhance tendon-bone healing. A total of 21 adult New Zealand white rabbits were used. The long extensor digitorum tendon was transplanted into a bone tunnel of the proximal tibia. The tendon was pulled through a drill hole in the proximal tibia and attached to the medial aspect of the tibia. Hydrogel suspension containing PSCs at a concentration of 20 million/mL was injected into the bone tunnel. Histological examination of the tendon-bone interface and biomechanical testing for maximal pull-out load were evaluated at postoperative weeks 3 and 6. Histological analysis of the tendon-bone interface showed an interface fibrous layer formed by the photoencapsulation of PSCs between the tendon and the bone. This layer became progressive integrated with the tendon-bone surface during the healing process. Biomechanical testing revealed higher maximal pull-out strength in experimental groups with a statistically significant difference at 3 and 6 weeks. These results suggest that photopolymerizable PEGDA and HA hydrogels are promising for tissue-engineered stem cell therapy to enhance tendon-bone healing.

KW - Hyaluronic acid

KW - Hydrogel

KW - Periosteal stem cells

KW - Photoencapsulation

KW - Tendon-bone healing

UR - http://www.scopus.com/inward/record.url?scp=34147188605&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34147188605&partnerID=8YFLogxK

U2 - 10.1142/S0218957706001820

DO - 10.1142/S0218957706001820

M3 - Article

VL - 10

SP - 109

EP - 120

JO - Journal of Musculoskeletal Research

JF - Journal of Musculoskeletal Research

SN - 0218-9577

IS - 3

ER -