Employing two distinct photonic crystal resonances to improve fluorescence enhancement

Patrick C. Mathias, Hsin Yu Wu, Brian T. Cunningham

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

Surface-bound fluorescence assays such as microarrays have emerged as a prominent technology in current life sciences research and are currently performed on optically passive substrates such as glass microscope slides. We present an alternative approach using photonic crystal substrates exhibiting resonant reflections. In this work, we design and fabricate a photonic crystal with a TM-polarized resonance at the cyanine-5 excitation wavelength and a TE-polarized resonance spectrally overlapping this fluorophore's emission spectrum. The former resonance increases the excitation of the fluorophore through enhanced electric field intensities, while the latter resonance redirects a proportion of emitted light toward the detection instrumentation. Spots of cyanine-5 conjugated streptavidin on the photonic crystal demonstrate a 60-fold increase in fluorescence intensity and a 42-fold increase in signal-to-noise ratio relative to a glass slide.

Original languageEnglish
Article number021111
JournalApplied Physics Letters
Volume95
Issue number2
DOIs
Publication statusPublished - 2009

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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