Emergence of D-aspartic acid in the differentiating neurons of the rat central nervous system

Kumiko Sakai, Hiroshi Homma, Jen Ai Lee, Takeshi Fukushima, Tomofumi Santa, Ken Tashiro, Takeshi Iwatsubo, Kazuhiro Imai

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72 Citations (Scopus)


The rat embryonic brain was probed with anti-D-aspartic acid (D-Asp) antiserum at different stages of development. At gestational day (E) 12, weak immunoreactivity (IR) of D-Asp was apparent at the hindbrain, midbrain and caudal forebrain, whereas it became more intense and extended over the whole brain at E20. However, IR markedly decreased after parturition. In the region of the immature forebrain at an early stage of development (E12), IR was mainly a characteristic of the cytoplasm of the neuronal cells, while in the more mature hindbrain it was localized in the axonal zone. In the more differentiated forebrain at a later stage of development (E18), the IR became restricted to zones which mainly consisted of axons and processes. Consequently, in the rat central nervous system; D-Asp first emerges during embryonic development as a feature of the cytoplasm and thereafter spreads into the axonal regions of neuronal cells, before disappearing almost completely after parturition.

Original languageEnglish
Pages (from-to)65-71
Number of pages7
JournalBrain Research
Issue number1
Publication statusPublished - Oct 12 1998
Externally publishedYes


  • Central nervous system
  • D-amino acid
  • D-aspartic acid
  • Neurogenesis
  • Neuron

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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