Elucidating the crucial role of poly N-acetylglucosamine from Staphylococcus aureus in cellular adhesion and pathogenesis

Mei Hui Lin, Jwu Ching Shu, Li Ping Lin, Kowit Yu Chong, Ya Wen Cheng, Jia Fu Du, Shih Tung Liu

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Staphylococcus aureus is an important pathogen that forms biofilms on the surfaces of medical implants. Biofilm formation by S. aureus is associated with the production of poly N-acetylglucosamine (PNAG), also referred to as polysaccharide intercellular adhesin (PIA), which mediates bacterial adhesion, leading to the accumulation of bacteria on solid surfaces. This study shows that the ability of S. aureus SA113 to adhere to nasal epithelial cells is reduced after the deletion of the ica operon, which contains genes encoding PIA/PNAG synthesis. However, this ability is restored after a plasmid carrying the entire ica operon is transformed into the mutant strain, S. aureus SA113Δica, showing that the synthesis of PIA/PNAG is important for adhesion to epithelial cells. Additionally, S. carnosus TM300, which does not produce PIA/PNAG, forms a biofilm and adheres to epithelial cells after the bacteria are transformed with a PIA/PNAG-expressing plasmid, pTXicaADBC. The adhesion of S. carnosus TM300 to epithelial cells is also demonstrated by adding purified exopolysaccharide (EPS), which contains PIA/PNAG, to the bacteria. In addition, using a mouse model, we find that the abscess lesions and bacterial burden in lung tissues is higher in mice infected with S. aureus SA113 than in those infected with the mutant strain, S. aureus-SA113Δica. The results indicate that PIA/PNAG promotes the adhesion of S. aureus to human nasal epithelial cells and lung infections in a mouse model. This study elucidates a mechanism that is important to the pathogenesis of S. aureus infections.

Original languageEnglish
Article numbere0124216
JournalPLoS One
Volume10
Issue number4
DOIs
Publication statusPublished - Apr 15 2015
Externally publishedYes

Fingerprint

N-acetylglucosamine
Acetylglucosamine
adhesins
cell adhesion
Staphylococcus aureus
Adhesion
pathogenesis
polysaccharides
epithelial cells
Epithelial Cells
Biofilms
Bacteria
biofilm
adhesion
operon
Operon
Nose
Plasmids
bacteria
plasmids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Lin, M. H., Shu, J. C., Lin, L. P., Chong, K. Y., Cheng, Y. W., Du, J. F., & Liu, S. T. (2015). Elucidating the crucial role of poly N-acetylglucosamine from Staphylococcus aureus in cellular adhesion and pathogenesis. PLoS One, 10(4), [e0124216]. https://doi.org/10.1371/journal.pone.0124216

Elucidating the crucial role of poly N-acetylglucosamine from Staphylococcus aureus in cellular adhesion and pathogenesis. / Lin, Mei Hui; Shu, Jwu Ching; Lin, Li Ping; Chong, Kowit Yu; Cheng, Ya Wen; Du, Jia Fu; Liu, Shih Tung.

In: PLoS One, Vol. 10, No. 4, e0124216, 15.04.2015.

Research output: Contribution to journalArticle

Lin, Mei Hui ; Shu, Jwu Ching ; Lin, Li Ping ; Chong, Kowit Yu ; Cheng, Ya Wen ; Du, Jia Fu ; Liu, Shih Tung. / Elucidating the crucial role of poly N-acetylglucosamine from Staphylococcus aureus in cellular adhesion and pathogenesis. In: PLoS One. 2015 ; Vol. 10, No. 4.
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