Elimination of cancer stem-like side population cells in hepatoma cell lines by Chinese herbal mixture Tien-Hsien Liquid

Chih Jung Yao, Chi-Tai Yeh, Liang Ming Lee, Shuang En Chuang, Chuan Feng Yeh, Wan Ju Chao, Tung-Iuan Lai, Gi Ming Lai

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Abstract

There are increasing pieces of evidence suggesting that the recurrence of cancer may result from a small subpopulation of cancer stem cells, which are resistant to the conventional chemotherapy and radiotherapy. We investigated the effects of Chinese herbal mixture Tien-Hsien Liquid (THL) on the cancer stem-like side population (SP) cells isolated from human hepatoma cells. After sorting and subsequent culture, the SP cells from Huh7 hepatoma cells appear to have higher clonogenicity and mRNA expressions of stemness genes such as SMO, ABCG2, CD133, β-catenin, and Oct-4 than those of non-SP cells. At dose of 2 mg/mL, THL reduced the proportion of SP cells in HepG2, Hep3B, and Huh7 cells from 1.33 to 0.49, 1.55 to 0.43, and 1.69 to 0.27, respectively. The viability and colony formation of Huh7 SP cells were effectively suppressed by THL dose-dependently, accompanied with the inhibition of stemness genes, e.g., ABCG2, CD133, and SMO. The tumorigenicity of THL-treated Huh7 SP cells in NOD/SCID mice was also diminished. Moreover, combination with THL could synergize the effect of doxorubicin against Huh7 SP cells. Our data indicate that THL may act as a cancer stem cell targeting therapeutics and be regarded as complementary and integrative medicine in the treatment of hepatoma.

Original languageEnglish
Article number617085
JournalEvidence-based Complementary and Alternative Medicine
Volume2012
DOIs
Publication statusPublished - 2012

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Side-Population Cells
Hepatocellular Carcinoma
Cell Line
Neoplasms
Neoplastic Stem Cells
Integrative Medicine
Catenins
Inbred NOD Mouse
SCID Mice
Complementary Therapies
Doxorubicin
tien-hsien liquid
Radiotherapy
Gene Expression
Recurrence
Drug Therapy
Messenger RNA
Therapeutics
Population
Genes

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Elimination of cancer stem-like side population cells in hepatoma cell lines by Chinese herbal mixture Tien-Hsien Liquid. / Yao, Chih Jung; Yeh, Chi-Tai; Lee, Liang Ming; Chuang, Shuang En; Yeh, Chuan Feng; Chao, Wan Ju; Lai, Tung-Iuan; Lai, Gi Ming.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2012, 617085, 2012.

Research output: Contribution to journalArticle

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