Elevated urinary d-lactate levels in patients with diabetes and microalbuminuria

Chu Kuang Chou, Ya Ting Lee, Shih Ming Chen, Chi Wen Hsieh, Tzu Chuan Huang, Yi Chieh Li, Jen Ai Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Diabetic nephropathy (DN) has become the major cause of end-stage renal disease. Early detection of disease risk, to enable intervention before advanced renal damage occurs, is an important goal. Microalbuminuria has been used to monitor renal damage in clinical settings for years. In this study, we divided patients with diabetes into different groups based on their microalbumin values to elucidate the relationship between urinary d-lactate and corresponding microalbumin values. Group DM1 comprised of patients with an albumin-to-creatinine ratio (ACR) of less than 30. μg albumin/mg creatinine (normal range); Group DM2 comprised of patients with an ACR of 30-299. μg albumin/mg creatinine (microalbuminuria); and Group DM3 comprised of patients with an ACR of ≥300. μg albumin/mg creatinine (macroalbuminuria). The urinary d-lactate concentration of patients with diabetes was determined by pre-column fluorescence derivatization with 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ), and the accuracy (recovery) and precision (relative standard deviation; RSD) were validated. The measured values showed an accuracy that was in the acceptable range (91.59-112.96%), with an RSD in the range of 3.13-13.21%. The urinary d-lactate levels of the 3 diabetic groups (groups DM1, DM2, and DM3) were significantly higher than those of healthy subjects (78.31. ±. 22.13, 92.47. ±. 21.98, and 47.29. ±. 17.51 vs. 6.28. ±. 2.39 nmol/mg creatinine, respectively; p

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume116
DOIs
Publication statusPublished - Jan 13 2015

Keywords

  • D-lactate
  • Diabetes
  • Fluorescence derivatization
  • Microalbumin
  • NBD-PZ

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Spectroscopy
  • Clinical Biochemistry

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