Elevated SRPK1 lessens apoptosis in breast cancer cells through RBM4-regulated splicing events

Jung Chun Lin, Ching Yu Lin, Woan Yuh Tarn, Fang Yu Li

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Imbalanced splicing of premessenger RNA is typical of tumorous malignancies, and the regulatory mechanisms involved in several tumorigenesis-associated splicing events are identified. Elevated expression of serine-arginine protein kinase 1 (SRPK1) may participate in the pathway responsible for the dysregulation of splicing events in malignant tumor cells. In this study, we observed a correlation between the cytoplasmic accumulation of RNA-binding motif protein 4 (RBM4) and up-regulated SRPK1 in breast cancer cells. The production of the IR-B and MCL-1Stranscripts was induced separately by the overexpression of RBM4 and SRPK1 gene silencing. Overexpressed RBM4 simultaneously bound to the CU-rich elements within the MCL-1 exon2 and the downstream intron, which subsequently facilitated the exclusion of the regulated exon. Breast cancer cells are deprived of apoptotic resistance through the RBM4-mediated up-regulation of the IR-B and MCL-1Stranscripts. These findings suggest that the splicing events regulated by the SRPK1-RMB4 network may contribute to tumorigenesis through altered sensitivity to apoptotic signals in breast cancer cells.

Original languageEnglish
Pages (from-to)1621-1631
Number of pages11
JournalRNA
Volume20
Issue number10
DOIs
Publication statusPublished - Oct 1 2014

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Keywords

  • Alternative splicing
  • Breast cancer
  • MCL-1
  • RNA binding motif protein 4

ASJC Scopus subject areas

  • Molecular Biology
  • Medicine(all)

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