Elevated plasma matrix metalloproteinase-9 protein and its gene polymorphism in patients with community-acquired pneumonia

Ting Yen Chiang, Ling Yuh Shyu, Thomas Chang Yao Tsao, Ming Hsien Chien, Shih Ming Tsao, Yuan Ti Lee, Shun Fa Yang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: The purpose here was to detect the association among plasma matrix metalloproteinase-9 (MMP-9) concentration, single nucleotide polymorphisms (SNPs) of MMP-9 gene and community-acquired pneumonia (CAP). Methods: The enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were, respectively used to measure the plasma MMP-9 level and its gene polymorphisms. Results: The level of plasma of MMP-9 was elevated in patients with CAP as compared to that of normal controls and decreased significantly after treatment. Plasma MMP-9 concentration was significantly correlated with white blood cell (WBC) and neutrophil counts in patients with CAP. No significant difference was found in the genotypes distribution of MMP-9 SNPs, rs3918242, rs17576 or rs2274756, between patients with CAP and normal controls. Plasma MMP-9 concentration was not associated with MMP-9 polymorphism. When the cut-off level of the plasma MMP-9 concentration was set to be 105.02 ng/mL, the odds ratio of plasma MMP-9 for CAP risk was 9.86 (95 confident interval: 4.2722.75). Plasma MMP-9 level may act as a prediction marker for CAP. Conclusions: Elevated plasma MMP-9 could be a biological marker for the diagnosis and be a new strategy for target therapy of community-acquired pneumonia.

Original languageEnglish
Pages (from-to)449-454
Number of pages6
JournalClinical Chemistry and Laboratory Medicine
Volume50
Issue number3
DOIs
Publication statusPublished - Mar 1 2012

Keywords

  • community-acquired pneumonia
  • gene polymorphism
  • metalloproteinase-9 (MMP-9)

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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