Elevated nitric oxide level in aqueous humor of patients with acute angle-closure glaucoma

Shih Hwa Chiou, Charn Jung Chang, Wen-Ming Hsu, Chung Lan Kao, Jorn Hon Liu, Wen Lu Chen, Der Chong Tsai, Chi Chiao Wu, Ching Kuang Chou

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Nitric oxide, a noxious and free-radical gas, plays a key role in vasodilatation, inflammation, immunity, and neurotoxicity. Studies have shown that a recently detected NO synthase inducible isoform, found in astrocytes in the optic nerve heads of glaucoma patients, could stimulate excessive production of NO. The purpose of our study was to elucidate the role of NO production in the pathogenesis of glaucoma. We measured the concentrations of NO in aqueous humor from 11 patients with acute angle-closure glaucoma (AACG) and 14 patients with cataract. All samples were collected from patients who were free of any other systemic disease. Utilizing a chemiluminescence assay, we found that in AACG patients the NO levels in aqueous humor samples (83.2 ± 6.7 μM) were significantly higher than in cataract patients (27.1 ± 3.6 μM; p < 0.001). We therefore concluded that excessive NO in AACG patients reflects the degree of ocular impairment demonstrated by concomitant retinal cell and optic nerve cell damage.

Original languageEnglish
Pages (from-to)113-116
Number of pages4
JournalOphthalmologica
Volume215
Issue number2
DOIs
Publication statusPublished - Mar 28 2001
Externally publishedYes

Fingerprint

Angle Closure Glaucoma
Aqueous Humor
Nitric Oxide
Glaucoma
Cataract
Optic Disk
Optic Nerve
Luminescence
Vasodilation
Nitric Oxide Synthase
Astrocytes
Free Radicals
Immunity
Protein Isoforms
Gases
Inflammation
Neurons

Keywords

  • Acute angle-closure glaucoma
  • Aqueous humor
  • Chemiluminescence
  • Nitric oxide

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Chiou, S. H., Chang, C. J., Hsu, W-M., Kao, C. L., Liu, J. H., Chen, W. L., ... Chou, C. K. (2001). Elevated nitric oxide level in aqueous humor of patients with acute angle-closure glaucoma. Ophthalmologica, 215(2), 113-116. https://doi.org/10.1159/000050840

Elevated nitric oxide level in aqueous humor of patients with acute angle-closure glaucoma. / Chiou, Shih Hwa; Chang, Charn Jung; Hsu, Wen-Ming; Kao, Chung Lan; Liu, Jorn Hon; Chen, Wen Lu; Tsai, Der Chong; Wu, Chi Chiao; Chou, Ching Kuang.

In: Ophthalmologica, Vol. 215, No. 2, 28.03.2001, p. 113-116.

Research output: Contribution to journalArticle

Chiou, SH, Chang, CJ, Hsu, W-M, Kao, CL, Liu, JH, Chen, WL, Tsai, DC, Wu, CC & Chou, CK 2001, 'Elevated nitric oxide level in aqueous humor of patients with acute angle-closure glaucoma', Ophthalmologica, vol. 215, no. 2, pp. 113-116. https://doi.org/10.1159/000050840
Chiou, Shih Hwa ; Chang, Charn Jung ; Hsu, Wen-Ming ; Kao, Chung Lan ; Liu, Jorn Hon ; Chen, Wen Lu ; Tsai, Der Chong ; Wu, Chi Chiao ; Chou, Ching Kuang. / Elevated nitric oxide level in aqueous humor of patients with acute angle-closure glaucoma. In: Ophthalmologica. 2001 ; Vol. 215, No. 2. pp. 113-116.
@article{854d6290ddf8417cac663c0d472fa787,
title = "Elevated nitric oxide level in aqueous humor of patients with acute angle-closure glaucoma",
abstract = "Nitric oxide, a noxious and free-radical gas, plays a key role in vasodilatation, inflammation, immunity, and neurotoxicity. Studies have shown that a recently detected NO synthase inducible isoform, found in astrocytes in the optic nerve heads of glaucoma patients, could stimulate excessive production of NO. The purpose of our study was to elucidate the role of NO production in the pathogenesis of glaucoma. We measured the concentrations of NO in aqueous humor from 11 patients with acute angle-closure glaucoma (AACG) and 14 patients with cataract. All samples were collected from patients who were free of any other systemic disease. Utilizing a chemiluminescence assay, we found that in AACG patients the NO levels in aqueous humor samples (83.2 ± 6.7 μM) were significantly higher than in cataract patients (27.1 ± 3.6 μM; p < 0.001). We therefore concluded that excessive NO in AACG patients reflects the degree of ocular impairment demonstrated by concomitant retinal cell and optic nerve cell damage.",
keywords = "Acute angle-closure glaucoma, Aqueous humor, Chemiluminescence, Nitric oxide",
author = "Chiou, {Shih Hwa} and Chang, {Charn Jung} and Wen-Ming Hsu and Kao, {Chung Lan} and Liu, {Jorn Hon} and Chen, {Wen Lu} and Tsai, {Der Chong} and Wu, {Chi Chiao} and Chou, {Ching Kuang}",
year = "2001",
month = "3",
day = "28",
doi = "10.1159/000050840",
language = "English",
volume = "215",
pages = "113--116",
journal = "Ophthalmologica",
issn = "0030-3755",
publisher = "S. Karger AG",
number = "2",

}

TY - JOUR

T1 - Elevated nitric oxide level in aqueous humor of patients with acute angle-closure glaucoma

AU - Chiou, Shih Hwa

AU - Chang, Charn Jung

AU - Hsu, Wen-Ming

AU - Kao, Chung Lan

AU - Liu, Jorn Hon

AU - Chen, Wen Lu

AU - Tsai, Der Chong

AU - Wu, Chi Chiao

AU - Chou, Ching Kuang

PY - 2001/3/28

Y1 - 2001/3/28

N2 - Nitric oxide, a noxious and free-radical gas, plays a key role in vasodilatation, inflammation, immunity, and neurotoxicity. Studies have shown that a recently detected NO synthase inducible isoform, found in astrocytes in the optic nerve heads of glaucoma patients, could stimulate excessive production of NO. The purpose of our study was to elucidate the role of NO production in the pathogenesis of glaucoma. We measured the concentrations of NO in aqueous humor from 11 patients with acute angle-closure glaucoma (AACG) and 14 patients with cataract. All samples were collected from patients who were free of any other systemic disease. Utilizing a chemiluminescence assay, we found that in AACG patients the NO levels in aqueous humor samples (83.2 ± 6.7 μM) were significantly higher than in cataract patients (27.1 ± 3.6 μM; p < 0.001). We therefore concluded that excessive NO in AACG patients reflects the degree of ocular impairment demonstrated by concomitant retinal cell and optic nerve cell damage.

AB - Nitric oxide, a noxious and free-radical gas, plays a key role in vasodilatation, inflammation, immunity, and neurotoxicity. Studies have shown that a recently detected NO synthase inducible isoform, found in astrocytes in the optic nerve heads of glaucoma patients, could stimulate excessive production of NO. The purpose of our study was to elucidate the role of NO production in the pathogenesis of glaucoma. We measured the concentrations of NO in aqueous humor from 11 patients with acute angle-closure glaucoma (AACG) and 14 patients with cataract. All samples were collected from patients who were free of any other systemic disease. Utilizing a chemiluminescence assay, we found that in AACG patients the NO levels in aqueous humor samples (83.2 ± 6.7 μM) were significantly higher than in cataract patients (27.1 ± 3.6 μM; p < 0.001). We therefore concluded that excessive NO in AACG patients reflects the degree of ocular impairment demonstrated by concomitant retinal cell and optic nerve cell damage.

KW - Acute angle-closure glaucoma

KW - Aqueous humor

KW - Chemiluminescence

KW - Nitric oxide

UR - http://www.scopus.com/inward/record.url?scp=0035100544&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035100544&partnerID=8YFLogxK

U2 - 10.1159/000050840

DO - 10.1159/000050840

M3 - Article

C2 - 11244341

AN - SCOPUS:0035100544

VL - 215

SP - 113

EP - 116

JO - Ophthalmologica

JF - Ophthalmologica

SN - 0030-3755

IS - 2

ER -