Elevated levels of liver methylglyoxal and d-lactate in early-stage hepatitis in rats

Wen Chuang Wang, Chu Kuang Chou, Ming Cheng Chuang, Yi Chieh Li, Jen Ai Lee

Research output: Contribution to journalArticle

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Abstract

Methylglyoxal (MGO) is highly cytotoxic and its levels are elevated in diabetes, nephropathy and atherosclerosis. However, it has never been studied in liver disease. For this reason, we aimed to assess the levels of MGO and its metabolite d-lactate in an early hepatitis model. Wistar rats were administered CCl4 (0.75mL/kg, i.p.) to induce hepatitis. In either CCl4-treated or untreated rats, alanine transaminase and aspartate transaminase levels did not change over the course of the study, indicating that significant liver damage did not occur following CCl4 treatment. However, the levels of MGO and d-lactate were higher in the livers of CCl4-treated animals than in untreated animals (MGO: 128.2±18.8 and 248.1±64.9μg/g protein, p<0.01; d-lactate: 0.860±0.040 and 1.293±0.078μmol/g protein, respectively p<0.01). Furthermore, in untreated and treated animals, serum d-lactate levels were 57.65±2.59 and 92.16±16.69μm and urine d-lactate levels were 1.060±0.007 and 1.555±0.366μmol/mg UCr, respectively (p<0.01). These data show that in this model of early-stage liver damage, the levels of MGO and its metabolite d-lactate are elevated and that d-lactate could be useful as a reference marker for the early stage of hepatitis.

Original languageEnglish
JournalBiomedical Chromatography
DOIs
Publication statusPublished - Feb 2018

Fingerprint

Pyruvaldehyde
Liver
Hepatitis
Rats
Lactic Acid
Animals
Metabolites
Medical problems
Aspartate Aminotransferases
Alanine Transaminase
Wistar Rats
Liver Diseases
Atherosclerosis
Proteins
Urine
Serum

Keywords

  • Biomarker
  • CCl-induced hepatitis
  • d-lactate
  • Liver methylglyoxal

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Cite this

Elevated levels of liver methylglyoxal and d-lactate in early-stage hepatitis in rats. / Wang, Wen Chuang; Chou, Chu Kuang; Chuang, Ming Cheng; Li, Yi Chieh; Lee, Jen Ai.

In: Biomedical Chromatography, 02.2018.

Research output: Contribution to journalArticle

Wang, Wen Chuang ; Chou, Chu Kuang ; Chuang, Ming Cheng ; Li, Yi Chieh ; Lee, Jen Ai. / Elevated levels of liver methylglyoxal and d-lactate in early-stage hepatitis in rats. In: Biomedical Chromatography. 2018.
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