Elevated expression of thyroid hormone receptor α2 (c-erb A-α2) in nasopharyngeal carcinoma

J. W. Lee, C. L. Chen, B. T. Juang, J. Y. Chen, C. S. Yang, S. L. Doong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Differential display was used to identify genes differentially expressed between cultured normal nasal epithelial cells and nasopharyngeal carcinoma (NPC) cell lines. A 130 bp cDNA fragment showing homology with thyroid hormone receptor α2 (TR-α2 or c-erb A-α2) was identified in NPC cell lines. Northern blot analysis using the 130 bp cDNA fragment and a TR-α2 specific cDNA containing part of the coding region as probes, we were able to detect a 2.7 kb transcript corresponding to that of TR-α2 in NPC cell lines but not in normal nasal epithelial cells. RNA in situ hybridization was used to detect TR-α2 expression in clinical biopsies obtained from NPC patients and non-tumour controls. TR-α2 mRNA was detected in 1 out of 24 (4.2%) normal nasopharynx epithelium biopsies, in 5 out of 27 (18.5%) primary and 15 out of 24 (62.5%) recurrent tumours. The positive rate of TR-α2 expression in recurrent NPC biopsies was significantly higher than that in normal nasopharynx epithelium (P

Original languageEnglish
Pages (from-to)1674-1680
Number of pages7
JournalBritish Journal of Cancer
Volume83
Issue number12
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Thyroid Hormone Receptors
varespladib methyl
Nasopharynx
Complementary DNA
Biopsy
Nose
Cell Line
Epithelium
Epithelial Cells
Northern Blotting
In Situ Hybridization
TR-2 mycotoxin
Nasopharyngeal carcinoma
RNA
Messenger RNA
Genes
Neoplasms

Keywords

  • C-erb A-α2 (TR-α2)
  • Differential display
  • Nasopharyngeal carcinoma
  • RNA in situ hybridization

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Elevated expression of thyroid hormone receptor α2 (c-erb A-α2) in nasopharyngeal carcinoma. / Lee, J. W.; Chen, C. L.; Juang, B. T.; Chen, J. Y.; Yang, C. S.; Doong, S. L.

In: British Journal of Cancer, Vol. 83, No. 12, 2000, p. 1674-1680.

Research output: Contribution to journalArticle

Lee, J. W. ; Chen, C. L. ; Juang, B. T. ; Chen, J. Y. ; Yang, C. S. ; Doong, S. L. / Elevated expression of thyroid hormone receptor α2 (c-erb A-α2) in nasopharyngeal carcinoma. In: British Journal of Cancer. 2000 ; Vol. 83, No. 12. pp. 1674-1680.
@article{a81943709e2b4458bd401346fa825f76,
title = "Elevated expression of thyroid hormone receptor α2 (c-erb A-α2) in nasopharyngeal carcinoma",
abstract = "Differential display was used to identify genes differentially expressed between cultured normal nasal epithelial cells and nasopharyngeal carcinoma (NPC) cell lines. A 130 bp cDNA fragment showing homology with thyroid hormone receptor α2 (TR-α2 or c-erb A-α2) was identified in NPC cell lines. Northern blot analysis using the 130 bp cDNA fragment and a TR-α2 specific cDNA containing part of the coding region as probes, we were able to detect a 2.7 kb transcript corresponding to that of TR-α2 in NPC cell lines but not in normal nasal epithelial cells. RNA in situ hybridization was used to detect TR-α2 expression in clinical biopsies obtained from NPC patients and non-tumour controls. TR-α2 mRNA was detected in 1 out of 24 (4.2{\%}) normal nasopharynx epithelium biopsies, in 5 out of 27 (18.5{\%}) primary and 15 out of 24 (62.5{\%}) recurrent tumours. The positive rate of TR-α2 expression in recurrent NPC biopsies was significantly higher than that in normal nasopharynx epithelium (P",
keywords = "C-erb A-α2 (TR-α2), Differential display, Nasopharyngeal carcinoma, RNA in situ hybridization",
author = "Lee, {J. W.} and Chen, {C. L.} and Juang, {B. T.} and Chen, {J. Y.} and Yang, {C. S.} and Doong, {S. L.}",
year = "2000",
doi = "10.1054/bjoc.2000.1505",
language = "English",
volume = "83",
pages = "1674--1680",
journal = "British Journal of Cancer",
issn = "0007-0920",
publisher = "Nature Publishing Group",
number = "12",

}

TY - JOUR

T1 - Elevated expression of thyroid hormone receptor α2 (c-erb A-α2) in nasopharyngeal carcinoma

AU - Lee, J. W.

AU - Chen, C. L.

AU - Juang, B. T.

AU - Chen, J. Y.

AU - Yang, C. S.

AU - Doong, S. L.

PY - 2000

Y1 - 2000

N2 - Differential display was used to identify genes differentially expressed between cultured normal nasal epithelial cells and nasopharyngeal carcinoma (NPC) cell lines. A 130 bp cDNA fragment showing homology with thyroid hormone receptor α2 (TR-α2 or c-erb A-α2) was identified in NPC cell lines. Northern blot analysis using the 130 bp cDNA fragment and a TR-α2 specific cDNA containing part of the coding region as probes, we were able to detect a 2.7 kb transcript corresponding to that of TR-α2 in NPC cell lines but not in normal nasal epithelial cells. RNA in situ hybridization was used to detect TR-α2 expression in clinical biopsies obtained from NPC patients and non-tumour controls. TR-α2 mRNA was detected in 1 out of 24 (4.2%) normal nasopharynx epithelium biopsies, in 5 out of 27 (18.5%) primary and 15 out of 24 (62.5%) recurrent tumours. The positive rate of TR-α2 expression in recurrent NPC biopsies was significantly higher than that in normal nasopharynx epithelium (P

AB - Differential display was used to identify genes differentially expressed between cultured normal nasal epithelial cells and nasopharyngeal carcinoma (NPC) cell lines. A 130 bp cDNA fragment showing homology with thyroid hormone receptor α2 (TR-α2 or c-erb A-α2) was identified in NPC cell lines. Northern blot analysis using the 130 bp cDNA fragment and a TR-α2 specific cDNA containing part of the coding region as probes, we were able to detect a 2.7 kb transcript corresponding to that of TR-α2 in NPC cell lines but not in normal nasal epithelial cells. RNA in situ hybridization was used to detect TR-α2 expression in clinical biopsies obtained from NPC patients and non-tumour controls. TR-α2 mRNA was detected in 1 out of 24 (4.2%) normal nasopharynx epithelium biopsies, in 5 out of 27 (18.5%) primary and 15 out of 24 (62.5%) recurrent tumours. The positive rate of TR-α2 expression in recurrent NPC biopsies was significantly higher than that in normal nasopharynx epithelium (P

KW - C-erb A-α2 (TR-α2)

KW - Differential display

KW - Nasopharyngeal carcinoma

KW - RNA in situ hybridization

UR - http://www.scopus.com/inward/record.url?scp=0034545152&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034545152&partnerID=8YFLogxK

U2 - 10.1054/bjoc.2000.1505

DO - 10.1054/bjoc.2000.1505

M3 - Article

C2 - 11104565

AN - SCOPUS:0034545152

VL - 83

SP - 1674

EP - 1680

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

IS - 12

ER -