Electroporation for three commonly used yeast strains for two-hybrid screening experiments

Hsin Ying Lin, Sey-En Lin, Su Fang Chien, Ming Kai Chern

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acetate (LiAc) and 50 mM Tris-HCl with 5 mM EDTA (pH 7.5), i.e., fivefold the standard concentrations, and voltage at 1.0 to develop a protocol which, for the first time, is able to effect an average efficiency of 1.84 × 106 transformants/μg DNA for three commonly used yeast strains committed to two-hybrid screening experiments.

Original languageEnglish
Pages (from-to)117-119
Number of pages3
JournalAnalytical Biochemistry
Volume416
Issue number1
DOIs
Publication statusPublished - Sep 1 2011

Fingerprint

Electroporation
Yeast
Screening
Yeasts
Edetic Acid
Experiments
DNA
Electric potential
lithium acetate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Electroporation for three commonly used yeast strains for two-hybrid screening experiments. / Lin, Hsin Ying; Lin, Sey-En; Chien, Su Fang; Chern, Ming Kai.

In: Analytical Biochemistry, Vol. 416, No. 1, 01.09.2011, p. 117-119.

Research output: Contribution to journalArticle

Lin, Hsin Ying ; Lin, Sey-En ; Chien, Su Fang ; Chern, Ming Kai. / Electroporation for three commonly used yeast strains for two-hybrid screening experiments. In: Analytical Biochemistry. 2011 ; Vol. 416, No. 1. pp. 117-119.
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