Electroporation for three commonly used yeast strains for two-hybrid screening experiments

Hsin Ying Lin, Sey-En Lin, Su Fang Chien, Ming Kai Chern

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acetate (LiAc) and 50 mM Tris-HCl with 5 mM EDTA (pH 7.5), i.e., fivefold the standard concentrations, and voltage at 1.0 to develop a protocol which, for the first time, is able to effect an average efficiency of 1.84 × 106 transformants/μg DNA for three commonly used yeast strains committed to two-hybrid screening experiments.

Original languageEnglish
Pages (from-to)117-119
Number of pages3
JournalAnalytical Biochemistry
Volume416
Issue number1
DOIs
Publication statusPublished - Sep 1 2011

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

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