Electrophysiological mapping and histological examinations of the swine atrium with sustained (≥24 h) atrial fibrillation

A suitable animal model for studying human atrial fibrillation

Jiunn Lee Lin, Ling Ping Lai, Chih Shen Lin, Chao Cheng Du, Tsu Juey Wu, Shih Ping Chen, Wen Chuan Lee, Ping Cheng Yang, Yung Zu Tseng, Wen Pin Lien, Shoei K.Stephen Huang

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Objectives: Interventional elimination of chronic persistent atrial fibrillation (AFib) remains difficult. An animal model mimicking the clinical situation is important. Methods and Results: Twenty-five adult pigs were implanted with a high-speed atrial pacemaker. After continuous pacing at 600 bpm for 6 weeks, 20 (91%) of the 22 survivals developed sustained AFib lasting for at least 24 h. Epicardial dense mapping revealed multiple coexisting reentrant wavelets in the left and the right atrium (LA and RA, respectively; 10.6 ± 2.9 vs. 7.6 ± 2.4 wavelets/cm2/s; p < 0.002). The mean local A-A intervals were 87.2 ± 14.6 ms in the LA and 103.3 ± 19.0 ms in the RA (p < 0.0002). Acute termination of sustained AFib was successful in 3 of the 5 pigs by propafenone, but in none of the 6 by dl-sotalol. Epicardial cryothermal ablation failed to terminate any AFib by compartmentalization of the RA free wall alone (4 pigs) or together with the LA appendage (4 pigs). Electron microscopic examination demonstrated diffuse perinuclear myolysis, myofibrillar fragmentation and mitochondrial crystal disruption in the atrium. Conclusions: Pacing-induced sustained AFib (≥ 24 h) in adult pigs is a feasible and efficient animal model with electrophysiological and histological characteristics closely similar to those seen in humans.

Original languageEnglish
Pages (from-to)78-84
Number of pages7
JournalCardiology
Volume99
Issue number2
DOIs
Publication statusPublished - May 6 2003
Externally publishedYes

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Atrial Fibrillation
Swine
Animal Models
Heart Atria
Epicardial Mapping
Propafenone
Sotalol
Electrons
Survival

Keywords

  • Animal model
  • Antiarrhythmic drugs
  • Atrial compartmentalization
  • Atrial fibrillation
  • Electrophysiological mapping

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pharmacology (medical)

Cite this

Electrophysiological mapping and histological examinations of the swine atrium with sustained (≥24 h) atrial fibrillation : A suitable animal model for studying human atrial fibrillation. / Lin, Jiunn Lee; Lai, Ling Ping; Lin, Chih Shen; Du, Chao Cheng; Wu, Tsu Juey; Chen, Shih Ping; Lee, Wen Chuan; Yang, Ping Cheng; Tseng, Yung Zu; Lien, Wen Pin; Huang, Shoei K.Stephen.

In: Cardiology, Vol. 99, No. 2, 06.05.2003, p. 78-84.

Research output: Contribution to journalArticle

Lin, Jiunn Lee ; Lai, Ling Ping ; Lin, Chih Shen ; Du, Chao Cheng ; Wu, Tsu Juey ; Chen, Shih Ping ; Lee, Wen Chuan ; Yang, Ping Cheng ; Tseng, Yung Zu ; Lien, Wen Pin ; Huang, Shoei K.Stephen. / Electrophysiological mapping and histological examinations of the swine atrium with sustained (≥24 h) atrial fibrillation : A suitable animal model for studying human atrial fibrillation. In: Cardiology. 2003 ; Vol. 99, No. 2. pp. 78-84.
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abstract = "Objectives: Interventional elimination of chronic persistent atrial fibrillation (AFib) remains difficult. An animal model mimicking the clinical situation is important. Methods and Results: Twenty-five adult pigs were implanted with a high-speed atrial pacemaker. After continuous pacing at 600 bpm for 6 weeks, 20 (91{\%}) of the 22 survivals developed sustained AFib lasting for at least 24 h. Epicardial dense mapping revealed multiple coexisting reentrant wavelets in the left and the right atrium (LA and RA, respectively; 10.6 ± 2.9 vs. 7.6 ± 2.4 wavelets/cm2/s; p < 0.002). The mean local A-A intervals were 87.2 ± 14.6 ms in the LA and 103.3 ± 19.0 ms in the RA (p < 0.0002). Acute termination of sustained AFib was successful in 3 of the 5 pigs by propafenone, but in none of the 6 by dl-sotalol. Epicardial cryothermal ablation failed to terminate any AFib by compartmentalization of the RA free wall alone (4 pigs) or together with the LA appendage (4 pigs). Electron microscopic examination demonstrated diffuse perinuclear myolysis, myofibrillar fragmentation and mitochondrial crystal disruption in the atrium. Conclusions: Pacing-induced sustained AFib (≥ 24 h) in adult pigs is a feasible and efficient animal model with electrophysiological and histological characteristics closely similar to those seen in humans.",
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T1 - Electrophysiological mapping and histological examinations of the swine atrium with sustained (≥24 h) atrial fibrillation

T2 - A suitable animal model for studying human atrial fibrillation

AU - Lin, Jiunn Lee

AU - Lai, Ling Ping

AU - Lin, Chih Shen

AU - Du, Chao Cheng

AU - Wu, Tsu Juey

AU - Chen, Shih Ping

AU - Lee, Wen Chuan

AU - Yang, Ping Cheng

AU - Tseng, Yung Zu

AU - Lien, Wen Pin

AU - Huang, Shoei K.Stephen

PY - 2003/5/6

Y1 - 2003/5/6

N2 - Objectives: Interventional elimination of chronic persistent atrial fibrillation (AFib) remains difficult. An animal model mimicking the clinical situation is important. Methods and Results: Twenty-five adult pigs were implanted with a high-speed atrial pacemaker. After continuous pacing at 600 bpm for 6 weeks, 20 (91%) of the 22 survivals developed sustained AFib lasting for at least 24 h. Epicardial dense mapping revealed multiple coexisting reentrant wavelets in the left and the right atrium (LA and RA, respectively; 10.6 ± 2.9 vs. 7.6 ± 2.4 wavelets/cm2/s; p < 0.002). The mean local A-A intervals were 87.2 ± 14.6 ms in the LA and 103.3 ± 19.0 ms in the RA (p < 0.0002). Acute termination of sustained AFib was successful in 3 of the 5 pigs by propafenone, but in none of the 6 by dl-sotalol. Epicardial cryothermal ablation failed to terminate any AFib by compartmentalization of the RA free wall alone (4 pigs) or together with the LA appendage (4 pigs). Electron microscopic examination demonstrated diffuse perinuclear myolysis, myofibrillar fragmentation and mitochondrial crystal disruption in the atrium. Conclusions: Pacing-induced sustained AFib (≥ 24 h) in adult pigs is a feasible and efficient animal model with electrophysiological and histological characteristics closely similar to those seen in humans.

AB - Objectives: Interventional elimination of chronic persistent atrial fibrillation (AFib) remains difficult. An animal model mimicking the clinical situation is important. Methods and Results: Twenty-five adult pigs were implanted with a high-speed atrial pacemaker. After continuous pacing at 600 bpm for 6 weeks, 20 (91%) of the 22 survivals developed sustained AFib lasting for at least 24 h. Epicardial dense mapping revealed multiple coexisting reentrant wavelets in the left and the right atrium (LA and RA, respectively; 10.6 ± 2.9 vs. 7.6 ± 2.4 wavelets/cm2/s; p < 0.002). The mean local A-A intervals were 87.2 ± 14.6 ms in the LA and 103.3 ± 19.0 ms in the RA (p < 0.0002). Acute termination of sustained AFib was successful in 3 of the 5 pigs by propafenone, but in none of the 6 by dl-sotalol. Epicardial cryothermal ablation failed to terminate any AFib by compartmentalization of the RA free wall alone (4 pigs) or together with the LA appendage (4 pigs). Electron microscopic examination demonstrated diffuse perinuclear myolysis, myofibrillar fragmentation and mitochondrial crystal disruption in the atrium. Conclusions: Pacing-induced sustained AFib (≥ 24 h) in adult pigs is a feasible and efficient animal model with electrophysiological and histological characteristics closely similar to those seen in humans.

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