Electrophoretic coating of amphiphilic chitosan colloids on regulating cellular behavior

Yen Jen Wang, Teng Yuan Lo, Chieh Hsi Wu, Dean Mo Liu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this communication, we report a facile nanotopographical control over a stainless steel surface via an electrophoretic deposition of colloidal amphiphilic chitosan for preferential growth, proliferation or migration of vascular smooth muscle cells (VSMCs) and human umbilical vein endothelial cells (HUVECs). Atomic force microscopy revealed that the colloidal surface exhibited a deposition time-dependent nanotopographical evolution, wherein two different nanotopographic textures indexed by 'kurtosis' (Rkur) value were easily designed, which were termed as 'sharp' (i.e. high peak-to-valley texture) surface and 'flat' (i.e. low peak-to-valley texture) surface. Cellular behaviour of VSMCs and HUVECs on both surfaces demonstrated topographically dependent morphogenesis, adherent responses and biochemical properties in comparison with bare stainless steel. The formation of a biofunctionalized surface upon a facile colloidal chitosan deposition envisions the potential application towards numerous biomedical devices, and this is especially promising for cardiovascular stents wherein a new surface with optimized texture can be designed and is expected to create an advantageous environment to stimulate HUVEC growth for improved healing performance.

Original languageEnglish
Article number0411
JournalJournal of the Royal Society Interface
Volume10
Issue number86
DOIs
Publication statusPublished - Sep 6 2013
Externally publishedYes

Fingerprint

Electrophoretic coatings
Human Umbilical Vein Endothelial Cells
Chitosan
Colloids
Stainless Steel
Vascular Smooth Muscle
Smooth Muscle Myocytes
Endothelial cells
Textures
Atomic Force Microscopy
Growth
Morphogenesis
Stents
Muscle
Stainless steel
Equipment and Supplies
Cells
Cell growth
Atomic force microscopy

Keywords

  • Biomimetic materials
  • Cell-specific growth
  • Colloidal chitosan
  • Electrophoretic deposition
  • Nanotopographic surface

ASJC Scopus subject areas

  • Biophysics
  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Biomaterials
  • Biochemistry
  • Medicine(all)

Cite this

Electrophoretic coating of amphiphilic chitosan colloids on regulating cellular behavior. / Wang, Yen Jen; Lo, Teng Yuan; Wu, Chieh Hsi; Liu, Dean Mo.

In: Journal of the Royal Society Interface, Vol. 10, No. 86, 0411, 06.09.2013.

Research output: Contribution to journalArticle

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