Electromechanical effects of 1,25-dihydroxyvitamin D with antiatrial fibrillation activities

Dicky A. Hanafy, Shih Lin Chang, Yen Yu Lu, Yao Chang Chen, Yu Hsun Kao, Jen Hung Huang, Shih Ann Chen, Yi Jen Chen

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Abstract

Electromechanical Effects of 1,25-Dihydroxyvitamin D on the LA Introduction Treatment with 1,25-dihydroxyvitamin D (1,25[OH]2D) has several cardiovascular benefits. 1,25[OH]2D has direct cellular effects, but its effects on the atrium are not clear. We evaluated the effects of 1,25[OH]2D on the atrial electrophysiology and atrial fibrillation (AF). Methods Conventional microelectrodes were used to record action potentials (APs) and contractility in isolated rabbit left atrium (LA) tissue preparations before and after the administration of 0.01, 0.1, and 1 nM 1,25[OH] 2D with and without rapid atrial pacing (RAP) and acetylcholine (5 mM)-induced AF. Surface ECG and intracardiac electrograms were recorded before and after the intravenous administration of 4 units/kg of 1,25[OH]2D in heart failure (HF) rabbits (4 weeks after coronary artery ligation) with RAP and acetylcholine-induced AF. Results 1,25[OH]2D dose-dependently increased the AP duration in the LA, which was abolished by pretreatment with 0.1 μM ryanodine. RAP and 5 mM acetylcholine-induced fewer (64.3% vs 100%, P <0.05) AF occurrences in the presence (n = 14) of 1,25[OH]2D than those (n = 14) in the absence of 1,25[OH]2D. The LA treated with 1,25[OH]2D (n = 9) had a slower maximal AF rate (10.9 ± 2.4 Hz vs 13.3 ± 2.7 Hz, P <0.05) than the LA (n = 14) without 1,25[OH]2D. Moreover, 1,25[OH]2D caused a lower AF inducible percentage (11.0 ± 1.9% vs 100 ± 0%, P <0.001) and a shorter duration (4 ± 0.4 seconds vs 309 ± 26 seconds, P <0.001) with a prolonged LA 90% monophasic AP duration (94.1 ± 0.2 milliseconds vs 98.5 ± 0.1 milliseconds, P <0.05) in 5 rabbits with HF. 1,25[OH]2D did not prolong the QT interval or 90% of the AP duration in isolated Purkinje fibers. Conclusion 1,25[OH]2D has direct electromechanical effects on the LA and can prevent or terminate AF.

Original languageEnglish
Pages (from-to)317-323
Number of pages7
JournalJournal of Cardiovascular Electrophysiology
Volume25
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Heart Atria
Atrial Fibrillation
Action Potentials
Acetylcholine
Rabbits
Heart Failure
Cardiac Electrophysiologic Techniques
Purkinje Fibers
Ryanodine
Electrophysiology
Microelectrodes
1,25-dihydroxyvitamin D
Intravenous Administration
Ligation
Coronary Vessels
Electrocardiography

Keywords

  • acetylcholine
  • atrial fibrillation
  • heart failure
  • left atrium
  • vitamin D

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)
  • Medicine(all)

Cite this

Electromechanical effects of 1,25-dihydroxyvitamin D with antiatrial fibrillation activities. / Hanafy, Dicky A.; Chang, Shih Lin; Lu, Yen Yu; Chen, Yao Chang; Kao, Yu Hsun; Huang, Jen Hung; Chen, Shih Ann; Chen, Yi Jen.

In: Journal of Cardiovascular Electrophysiology, Vol. 25, No. 3, 2014, p. 317-323.

Research output: Contribution to journalArticle

Hanafy, Dicky A. ; Chang, Shih Lin ; Lu, Yen Yu ; Chen, Yao Chang ; Kao, Yu Hsun ; Huang, Jen Hung ; Chen, Shih Ann ; Chen, Yi Jen. / Electromechanical effects of 1,25-dihydroxyvitamin D with antiatrial fibrillation activities. In: Journal of Cardiovascular Electrophysiology. 2014 ; Vol. 25, No. 3. pp. 317-323.
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abstract = "Electromechanical Effects of 1,25-Dihydroxyvitamin D on the LA Introduction Treatment with 1,25-dihydroxyvitamin D (1,25[OH]2D) has several cardiovascular benefits. 1,25[OH]2D has direct cellular effects, but its effects on the atrium are not clear. We evaluated the effects of 1,25[OH]2D on the atrial electrophysiology and atrial fibrillation (AF). Methods Conventional microelectrodes were used to record action potentials (APs) and contractility in isolated rabbit left atrium (LA) tissue preparations before and after the administration of 0.01, 0.1, and 1 nM 1,25[OH] 2D with and without rapid atrial pacing (RAP) and acetylcholine (5 mM)-induced AF. Surface ECG and intracardiac electrograms were recorded before and after the intravenous administration of 4 units/kg of 1,25[OH]2D in heart failure (HF) rabbits (4 weeks after coronary artery ligation) with RAP and acetylcholine-induced AF. Results 1,25[OH]2D dose-dependently increased the AP duration in the LA, which was abolished by pretreatment with 0.1 μM ryanodine. RAP and 5 mM acetylcholine-induced fewer (64.3{\%} vs 100{\%}, P <0.05) AF occurrences in the presence (n = 14) of 1,25[OH]2D than those (n = 14) in the absence of 1,25[OH]2D. The LA treated with 1,25[OH]2D (n = 9) had a slower maximal AF rate (10.9 ± 2.4 Hz vs 13.3 ± 2.7 Hz, P <0.05) than the LA (n = 14) without 1,25[OH]2D. Moreover, 1,25[OH]2D caused a lower AF inducible percentage (11.0 ± 1.9{\%} vs 100 ± 0{\%}, P <0.001) and a shorter duration (4 ± 0.4 seconds vs 309 ± 26 seconds, P <0.001) with a prolonged LA 90{\%} monophasic AP duration (94.1 ± 0.2 milliseconds vs 98.5 ± 0.1 milliseconds, P <0.05) in 5 rabbits with HF. 1,25[OH]2D did not prolong the QT interval or 90{\%} of the AP duration in isolated Purkinje fibers. Conclusion 1,25[OH]2D has direct electromechanical effects on the LA and can prevent or terminate AF.",
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AU - Hanafy, Dicky A.

AU - Chang, Shih Lin

AU - Lu, Yen Yu

AU - Chen, Yao Chang

AU - Kao, Yu Hsun

AU - Huang, Jen Hung

AU - Chen, Shih Ann

AU - Chen, Yi Jen

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N2 - Electromechanical Effects of 1,25-Dihydroxyvitamin D on the LA Introduction Treatment with 1,25-dihydroxyvitamin D (1,25[OH]2D) has several cardiovascular benefits. 1,25[OH]2D has direct cellular effects, but its effects on the atrium are not clear. We evaluated the effects of 1,25[OH]2D on the atrial electrophysiology and atrial fibrillation (AF). Methods Conventional microelectrodes were used to record action potentials (APs) and contractility in isolated rabbit left atrium (LA) tissue preparations before and after the administration of 0.01, 0.1, and 1 nM 1,25[OH] 2D with and without rapid atrial pacing (RAP) and acetylcholine (5 mM)-induced AF. Surface ECG and intracardiac electrograms were recorded before and after the intravenous administration of 4 units/kg of 1,25[OH]2D in heart failure (HF) rabbits (4 weeks after coronary artery ligation) with RAP and acetylcholine-induced AF. Results 1,25[OH]2D dose-dependently increased the AP duration in the LA, which was abolished by pretreatment with 0.1 μM ryanodine. RAP and 5 mM acetylcholine-induced fewer (64.3% vs 100%, P <0.05) AF occurrences in the presence (n = 14) of 1,25[OH]2D than those (n = 14) in the absence of 1,25[OH]2D. The LA treated with 1,25[OH]2D (n = 9) had a slower maximal AF rate (10.9 ± 2.4 Hz vs 13.3 ± 2.7 Hz, P <0.05) than the LA (n = 14) without 1,25[OH]2D. Moreover, 1,25[OH]2D caused a lower AF inducible percentage (11.0 ± 1.9% vs 100 ± 0%, P <0.001) and a shorter duration (4 ± 0.4 seconds vs 309 ± 26 seconds, P <0.001) with a prolonged LA 90% monophasic AP duration (94.1 ± 0.2 milliseconds vs 98.5 ± 0.1 milliseconds, P <0.05) in 5 rabbits with HF. 1,25[OH]2D did not prolong the QT interval or 90% of the AP duration in isolated Purkinje fibers. Conclusion 1,25[OH]2D has direct electromechanical effects on the LA and can prevent or terminate AF.

AB - Electromechanical Effects of 1,25-Dihydroxyvitamin D on the LA Introduction Treatment with 1,25-dihydroxyvitamin D (1,25[OH]2D) has several cardiovascular benefits. 1,25[OH]2D has direct cellular effects, but its effects on the atrium are not clear. We evaluated the effects of 1,25[OH]2D on the atrial electrophysiology and atrial fibrillation (AF). Methods Conventional microelectrodes were used to record action potentials (APs) and contractility in isolated rabbit left atrium (LA) tissue preparations before and after the administration of 0.01, 0.1, and 1 nM 1,25[OH] 2D with and without rapid atrial pacing (RAP) and acetylcholine (5 mM)-induced AF. Surface ECG and intracardiac electrograms were recorded before and after the intravenous administration of 4 units/kg of 1,25[OH]2D in heart failure (HF) rabbits (4 weeks after coronary artery ligation) with RAP and acetylcholine-induced AF. Results 1,25[OH]2D dose-dependently increased the AP duration in the LA, which was abolished by pretreatment with 0.1 μM ryanodine. RAP and 5 mM acetylcholine-induced fewer (64.3% vs 100%, P <0.05) AF occurrences in the presence (n = 14) of 1,25[OH]2D than those (n = 14) in the absence of 1,25[OH]2D. The LA treated with 1,25[OH]2D (n = 9) had a slower maximal AF rate (10.9 ± 2.4 Hz vs 13.3 ± 2.7 Hz, P <0.05) than the LA (n = 14) without 1,25[OH]2D. Moreover, 1,25[OH]2D caused a lower AF inducible percentage (11.0 ± 1.9% vs 100 ± 0%, P <0.001) and a shorter duration (4 ± 0.4 seconds vs 309 ± 26 seconds, P <0.001) with a prolonged LA 90% monophasic AP duration (94.1 ± 0.2 milliseconds vs 98.5 ± 0.1 milliseconds, P <0.05) in 5 rabbits with HF. 1,25[OH]2D did not prolong the QT interval or 90% of the AP duration in isolated Purkinje fibers. Conclusion 1,25[OH]2D has direct electromechanical effects on the LA and can prevent or terminate AF.

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