Efficient generation of hepatic cells from mesenchymal stromal cells by an innovative bio-microfluidic cell culture device

Meng Hua Yen, Yuan Yi Wu, Yi Shiuan Liu, Marilyn Rimando, Jennifer Hui Chun Ho, Oscar Kuang Sheng Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Mesenchymal stromal cells (MSCs) are multipotent and have great potential in cell therapy. Previously we reported the differentiation potential of human MSCs into hepatocytes in vitro and that these cells can rescue fulminant hepatic failure. However, the conventional static culture method neither maintains growth factors at an optimal level constantly nor removes cellular waste efficiently. In addition, not only is the duration of differentiating hepatocyte lineage cells from MSCs required to improve, but also the need for a large number of hepatocytes for cell therapy has not to date been addressed fully. The purpose of this study is to design and develop an innovative microfluidic device to overcome these shortcomings. Methods: We designed and fabricated a microfluidic device and a culture system for hepatic differentiation of MSCs using our protocol reported previously. The microfluidic device contains a large culture chamber with a stable uniform flow to allow homogeneous distribution and expansion as well as efficient induction of hepatic differentiation for MSCs. Results: The device enables real-time observation under light microscopy and exhibits a better differentiation efficiency for MSCs compared with conventional static culture. MSCs grown in the microfluidic device showed a higher level of hepatocyte marker gene expression under hepatic induction. Functional analysis of hepatic differentiation demonstrated significantly higher urea production in the microfluidic device after 21 days of hepatic differentiation. Conclusions: The microfluidic device allows the generation of a large number of MSCs and induces hepatic differentiation of MSCs efficiently. The device can be adapted for scale-up production of hepatic cells from MSCs for cellular therapy.

Original languageEnglish
Article number120
JournalStem Cell Research and Therapy
Volume7
Issue number1
DOIs
Publication statusPublished - Aug 19 2016

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Lab-On-A-Chip Devices
Microfluidics
Mesenchymal Stromal Cells
Cell culture
Hepatocytes
Cell Culture Techniques
Equipment and Supplies
Liver
Cell- and Tissue-Based Therapy
Differentiation (calculus)
Functional analysis
Gene expression
Optical microscopy
Urea
Intercellular Signaling Peptides and Proteins
Acute Liver Failure
Microscopy
Observation
Gene Expression
Light

Keywords

  • Hepatic differentiation
  • Hepatocyte
  • Mesenchymal stromal cells
  • Microfluidic device

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Molecular Medicine
  • Cell Biology
  • Medicine (miscellaneous)

Cite this

Efficient generation of hepatic cells from mesenchymal stromal cells by an innovative bio-microfluidic cell culture device. / Yen, Meng Hua; Wu, Yuan Yi; Liu, Yi Shiuan; Rimando, Marilyn; Ho, Jennifer Hui Chun; Lee, Oscar Kuang Sheng.

In: Stem Cell Research and Therapy, Vol. 7, No. 1, 120, 19.08.2016.

Research output: Contribution to journalArticle

Yen, Meng Hua ; Wu, Yuan Yi ; Liu, Yi Shiuan ; Rimando, Marilyn ; Ho, Jennifer Hui Chun ; Lee, Oscar Kuang Sheng. / Efficient generation of hepatic cells from mesenchymal stromal cells by an innovative bio-microfluidic cell culture device. In: Stem Cell Research and Therapy. 2016 ; Vol. 7, No. 1.
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