Abstract

Inhalation of zinc oxide (ZnO) metal fumes is known to cause metal fume fever and to have systemic effects; however, the effects of ZnO nanoparticles (ZnONPs) on the cardiovascular system remain unclear. The objective of this study was to investigate the cardiovascular toxicity of ZnONPs. Human coronary artery endothelial cells (HCAECs) were exposed to ZnONPs of different sizes to investigate the cell viability, 8-hydroxy-2'-deoxyguanosine (8-OHdG), interleukin (IL)-6, nitric oxide (NO), and regulation of cardiovascular disease-related genes. Exposure of HCAECs to ZnONPs resulted in decreased cell viability and increased levels of 8-OHdG, IL-6, and NO. Downregulation of cardiovascular-associated genes was observed in response to ZnONPs in HCAECs determined by qPCR, suggesting that the calcium signaling pathway, neuroactive ligand-receptor interaction, hypertrophic cardiomyopathy, dilated cardiomyopathy, and renin-angiotensin system are important affected pathways in response to ZnONPs. Furthermore, we observed a significant response of AGTR1 to ZnONP exposure in HCAECs. Our results suggest that ZnONPs cause toxicity to HCAECs, which could be associated with cardiovascular dysfunction.

Original languageEnglish
Pages (from-to)138-144
Number of pages7
JournalFood and Chemical Toxicology
Volume93
DOIs
Publication statusPublished - Jul 1 2016

Fingerprint

Zinc Oxide
zinc oxide
Endothelial cells
coronary vessels
nanoparticles
Nanoparticles
endothelial cells
Coronary Vessels
Endothelial Cells
Fumes
cardiomyopathy
interleukin-6
cell viability
nitric oxide
Toxicity
Interleukin-6
Cell Survival
Nitric Oxide
Genes
Metals

Keywords

  • AGTR1
  • Inflammation
  • Nanoparticles
  • Nitric oxide
  • Oxidative stress

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Effects of zinc oxide nanoparticles on human coronary artery endothelial cells. / Chuang, Kai-Jen; Lee, Kang-Yun; Pan, Chih Hong; Lai, Ching Huang; Lin, Lian Yu; Ho, Shu-Chuan; Ho, K. F.; Chuang, Hsiao-Chi.

In: Food and Chemical Toxicology, Vol. 93, 01.07.2016, p. 138-144.

Research output: Contribution to journalArticle

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AU - Ho, K. F.

AU - Chuang, Hsiao-Chi

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