Abstract

The yam tuber is a traditional Chinese medicine used in long-term treatment as a juvenescent substance. The purified yam tuber's major water-soluble protein, dioscorin, and its protease hydrolysates have been reported to have several biological activities. In this study, d-galactose (Gal) was subcutaneously injected into the dorsal necks of BALB/c mice daily for 10. weeks (Gal group) to induce oxidative stress. By the fifth week, 20 or 80. mg dioscorin/kg was orally administered daily combined with a daily Gal injection until the end of the study. The plasma malondialdehyde (MDA) level and advanced glycation end-products obtained after dioscorin oral administrations were lower compared to the Gal group. In addition, the latency and swimming distance in the mice that received dioscorin administration were significantly improved compared to the Gal group in the Morris water maze. Dioscorin administration resulted in higher GSH levels and oxygen radical antioxidant capacity (ORAC) activity and lower MDA and inducible nitric oxide synthase (iNOS) levels in the brain compared to mice in the Gal group. These elevated antioxidant activities following oral administration of yam dioscorin in vivo may reflect traditional juvenescent uses with the potential for anti-aging treatments.

Original languageEnglish
Pages (from-to)356-363
Number of pages8
JournalFood and Chemical Toxicology
Volume65
DOIs
Publication statusPublished - Mar 2014

Fingerprint

Dioscorea
yams
Galactose
galactose
tubers
learning
Learning
mice
Proteins
proteins
Malondialdehyde
malondialdehyde
oral administration
Oral Administration
Antioxidants
Advanced Glycosylation End Products
Oxidative stress
Water
Chinese Traditional Medicine
Nitric Oxide Synthase Type II

Keywords

  • D-Galactose-induced oxidative damage
  • Dioscorin
  • Malondialdehyde (MDA)
  • Morris water maze
  • Yam tuber

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

@article{50690e00d70f49b585f3e25aed58fba6,
title = "Effects of yam tuber protein, dioscorin, on attenuating oxidative status and learning dysfunction in d-galactose-induced BALB/c mice",
abstract = "The yam tuber is a traditional Chinese medicine used in long-term treatment as a juvenescent substance. The purified yam tuber's major water-soluble protein, dioscorin, and its protease hydrolysates have been reported to have several biological activities. In this study, d-galactose (Gal) was subcutaneously injected into the dorsal necks of BALB/c mice daily for 10. weeks (Gal group) to induce oxidative stress. By the fifth week, 20 or 80. mg dioscorin/kg was orally administered daily combined with a daily Gal injection until the end of the study. The plasma malondialdehyde (MDA) level and advanced glycation end-products obtained after dioscorin oral administrations were lower compared to the Gal group. In addition, the latency and swimming distance in the mice that received dioscorin administration were significantly improved compared to the Gal group in the Morris water maze. Dioscorin administration resulted in higher GSH levels and oxygen radical antioxidant capacity (ORAC) activity and lower MDA and inducible nitric oxide synthase (iNOS) levels in the brain compared to mice in the Gal group. These elevated antioxidant activities following oral administration of yam dioscorin in vivo may reflect traditional juvenescent uses with the potential for anti-aging treatments.",
keywords = "D-Galactose-induced oxidative damage, Dioscorin, Malondialdehyde (MDA), Morris water maze, Yam tuber",
author = "Han, {Chuan Hsiao} and Lin, {Yuh Feng} and Lin, {Yin Shiou} and Lee, {Tai Lin} and Huang, {Wei Jan} and Lin, {Shyr Yi} and Hou, {Wen Chi}",
year = "2014",
month = "3",
doi = "10.1016/j.fct.2014.01.012",
language = "English",
volume = "65",
pages = "356--363",
journal = "Food and Chemical Toxicology",
issn = "0278-6915",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Effects of yam tuber protein, dioscorin, on attenuating oxidative status and learning dysfunction in d-galactose-induced BALB/c mice

AU - Han, Chuan Hsiao

AU - Lin, Yuh Feng

AU - Lin, Yin Shiou

AU - Lee, Tai Lin

AU - Huang, Wei Jan

AU - Lin, Shyr Yi

AU - Hou, Wen Chi

PY - 2014/3

Y1 - 2014/3

N2 - The yam tuber is a traditional Chinese medicine used in long-term treatment as a juvenescent substance. The purified yam tuber's major water-soluble protein, dioscorin, and its protease hydrolysates have been reported to have several biological activities. In this study, d-galactose (Gal) was subcutaneously injected into the dorsal necks of BALB/c mice daily for 10. weeks (Gal group) to induce oxidative stress. By the fifth week, 20 or 80. mg dioscorin/kg was orally administered daily combined with a daily Gal injection until the end of the study. The plasma malondialdehyde (MDA) level and advanced glycation end-products obtained after dioscorin oral administrations were lower compared to the Gal group. In addition, the latency and swimming distance in the mice that received dioscorin administration were significantly improved compared to the Gal group in the Morris water maze. Dioscorin administration resulted in higher GSH levels and oxygen radical antioxidant capacity (ORAC) activity and lower MDA and inducible nitric oxide synthase (iNOS) levels in the brain compared to mice in the Gal group. These elevated antioxidant activities following oral administration of yam dioscorin in vivo may reflect traditional juvenescent uses with the potential for anti-aging treatments.

AB - The yam tuber is a traditional Chinese medicine used in long-term treatment as a juvenescent substance. The purified yam tuber's major water-soluble protein, dioscorin, and its protease hydrolysates have been reported to have several biological activities. In this study, d-galactose (Gal) was subcutaneously injected into the dorsal necks of BALB/c mice daily for 10. weeks (Gal group) to induce oxidative stress. By the fifth week, 20 or 80. mg dioscorin/kg was orally administered daily combined with a daily Gal injection until the end of the study. The plasma malondialdehyde (MDA) level and advanced glycation end-products obtained after dioscorin oral administrations were lower compared to the Gal group. In addition, the latency and swimming distance in the mice that received dioscorin administration were significantly improved compared to the Gal group in the Morris water maze. Dioscorin administration resulted in higher GSH levels and oxygen radical antioxidant capacity (ORAC) activity and lower MDA and inducible nitric oxide synthase (iNOS) levels in the brain compared to mice in the Gal group. These elevated antioxidant activities following oral administration of yam dioscorin in vivo may reflect traditional juvenescent uses with the potential for anti-aging treatments.

KW - D-Galactose-induced oxidative damage

KW - Dioscorin

KW - Malondialdehyde (MDA)

KW - Morris water maze

KW - Yam tuber

UR - http://www.scopus.com/inward/record.url?scp=84893385102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893385102&partnerID=8YFLogxK

U2 - 10.1016/j.fct.2014.01.012

DO - 10.1016/j.fct.2014.01.012

M3 - Article

C2 - 24440430

AN - SCOPUS:84893385102

VL - 65

SP - 356

EP - 363

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

SN - 0278-6915

ER -