Effects of tuber storage protein of yam (Dioscorea alata cv. Tainong No. 1) and its peptic hydrolyzates on spontaneously hypertensive rats

Chien Liang Lin, Shyr Yi Lin, Yaw Huei Lin, Wen Chi Hou

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33 Citations (Scopus)

Abstract

Yam storage protein (YSP) was purified from tubers of Dioscorea alata L. Tainong No. 1 (TN1) to homogeneity by DE-52 ion-exchange chromatography. The short-term (24 h) and long-term (25 days) antihypertensive effects of YSP-TN1 and its peptic hydrolyzates (PH-TN1) were measured in spontaneously hypertensive rats (SHRs). For 24-h antihypertensive measurements, SHRs (age 10 weeks, body weight from 240 to 250 g) were administered orally once (YSP-TN1 and PH-TN1, 40 mg kg-1 SHR) to measure the mean blood pressure (MBP), systolic blood pressure (SBP) and diastolic blood pressure (DBP). For a long-term antihypertensive measurement, SHRs (age 12 weeks, body weight from 250 to 270 g) were administered orally once a day for 25 days (YSP-TN1, 40 mg kg-1 SHR) to measure SBP, DBP and MBP. Captopril (10 or 15 mg kg-1 SHR) was used as a positive control. It was found that short-term administration of 40 mg kg-1 SHR of YSP-TN1l and PH-TN1 effectively lowered SHRs' MBP, SBP and DBP (For YSP-TN1, the lowest blood pressure was reached in the fourth hour and for PH-TN1 in the eighth hour). The lasting effects of PH-TN1 on reduced SHRs' BP were better than those of YSP-TN1 for one oral administration. For oral administration of 40 mg YSP-TN1 kg-1 SHR, the reduced MBP was 21.5 mmHg, which was comparable to 25.2 mmHg (the fourth hour) of 10 mg captopril kg-1 SHR oral administration. For oral administration of 40 mg PH-TN1 kg-1 SHR, the reduced MBP was 33.7 mmHg, comparable to 38.4 mmHg (the fourth hour) of 15 mg captopril kg-1 SHR. For long-term 25-day oral administration of 40 mg YSP-TN1 kg-1 SHR once a day, it was found that a feeding trial of YSP-TN1 effectively lowered SHRs' SBP, DBP and MBP. The greatest reduction in SHRs' blood pressure was reached on the ninth day, for the reduced SBP, 27.7 mmHg; for the reduced DBP, 28.3 mmHg; and for the reduced MBP, 27.5 mmHg.

Original languageEnglish
Pages (from-to)1489-1494
Number of pages6
JournalJournal of the Science of Food and Agriculture
Volume86
Issue number10
DOIs
Publication statusPublished - Aug 15 2006

Fingerprint

Dioscorea alata
Dioscorea
yams
Blood pressure
Inbred SHR Rats
storage proteins
Rats
Digestion
tubers
digestion
Blood Pressure
rats
blood pressure
Proteins
diastolic blood pressure
systolic blood pressure
oral administration
Captopril
Oral Administration
antihypertensive agents

Keywords

  • Antihypertension
  • Diastolic blood pressure (DBP)
  • Means of blood pressure (MBP)
  • Peptic hydrolyzates
  • Spontaneously hypertensive rat (SHR)
  • Storage protein
  • Systolic blood pressure (SBP)
  • Yam

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

@article{a95dc0ab34574c90b51fc5ce04794383,
title = "Effects of tuber storage protein of yam (Dioscorea alata cv. Tainong No. 1) and its peptic hydrolyzates on spontaneously hypertensive rats",
abstract = "Yam storage protein (YSP) was purified from tubers of Dioscorea alata L. Tainong No. 1 (TN1) to homogeneity by DE-52 ion-exchange chromatography. The short-term (24 h) and long-term (25 days) antihypertensive effects of YSP-TN1 and its peptic hydrolyzates (PH-TN1) were measured in spontaneously hypertensive rats (SHRs). For 24-h antihypertensive measurements, SHRs (age 10 weeks, body weight from 240 to 250 g) were administered orally once (YSP-TN1 and PH-TN1, 40 mg kg-1 SHR) to measure the mean blood pressure (MBP), systolic blood pressure (SBP) and diastolic blood pressure (DBP). For a long-term antihypertensive measurement, SHRs (age 12 weeks, body weight from 250 to 270 g) were administered orally once a day for 25 days (YSP-TN1, 40 mg kg-1 SHR) to measure SBP, DBP and MBP. Captopril (10 or 15 mg kg-1 SHR) was used as a positive control. It was found that short-term administration of 40 mg kg-1 SHR of YSP-TN1l and PH-TN1 effectively lowered SHRs' MBP, SBP and DBP (For YSP-TN1, the lowest blood pressure was reached in the fourth hour and for PH-TN1 in the eighth hour). The lasting effects of PH-TN1 on reduced SHRs' BP were better than those of YSP-TN1 for one oral administration. For oral administration of 40 mg YSP-TN1 kg-1 SHR, the reduced MBP was 21.5 mmHg, which was comparable to 25.2 mmHg (the fourth hour) of 10 mg captopril kg-1 SHR oral administration. For oral administration of 40 mg PH-TN1 kg-1 SHR, the reduced MBP was 33.7 mmHg, comparable to 38.4 mmHg (the fourth hour) of 15 mg captopril kg-1 SHR. For long-term 25-day oral administration of 40 mg YSP-TN1 kg-1 SHR once a day, it was found that a feeding trial of YSP-TN1 effectively lowered SHRs' SBP, DBP and MBP. The greatest reduction in SHRs' blood pressure was reached on the ninth day, for the reduced SBP, 27.7 mmHg; for the reduced DBP, 28.3 mmHg; and for the reduced MBP, 27.5 mmHg.",
keywords = "Antihypertension, Diastolic blood pressure (DBP), Means of blood pressure (MBP), Peptic hydrolyzates, Spontaneously hypertensive rat (SHR), Storage protein, Systolic blood pressure (SBP), Yam",
author = "Lin, {Chien Liang} and Lin, {Shyr Yi} and Lin, {Yaw Huei} and Hou, {Wen Chi}",
year = "2006",
month = "8",
day = "15",
doi = "10.1002/jsfa.2530",
language = "English",
volume = "86",
pages = "1489--1494",
journal = "Journal of the Science of Food and Agriculture",
issn = "0022-5142",
publisher = "John Wiley and Sons Ltd",
number = "10",

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TY - JOUR

T1 - Effects of tuber storage protein of yam (Dioscorea alata cv. Tainong No. 1) and its peptic hydrolyzates on spontaneously hypertensive rats

AU - Lin, Chien Liang

AU - Lin, Shyr Yi

AU - Lin, Yaw Huei

AU - Hou, Wen Chi

PY - 2006/8/15

Y1 - 2006/8/15

N2 - Yam storage protein (YSP) was purified from tubers of Dioscorea alata L. Tainong No. 1 (TN1) to homogeneity by DE-52 ion-exchange chromatography. The short-term (24 h) and long-term (25 days) antihypertensive effects of YSP-TN1 and its peptic hydrolyzates (PH-TN1) were measured in spontaneously hypertensive rats (SHRs). For 24-h antihypertensive measurements, SHRs (age 10 weeks, body weight from 240 to 250 g) were administered orally once (YSP-TN1 and PH-TN1, 40 mg kg-1 SHR) to measure the mean blood pressure (MBP), systolic blood pressure (SBP) and diastolic blood pressure (DBP). For a long-term antihypertensive measurement, SHRs (age 12 weeks, body weight from 250 to 270 g) were administered orally once a day for 25 days (YSP-TN1, 40 mg kg-1 SHR) to measure SBP, DBP and MBP. Captopril (10 or 15 mg kg-1 SHR) was used as a positive control. It was found that short-term administration of 40 mg kg-1 SHR of YSP-TN1l and PH-TN1 effectively lowered SHRs' MBP, SBP and DBP (For YSP-TN1, the lowest blood pressure was reached in the fourth hour and for PH-TN1 in the eighth hour). The lasting effects of PH-TN1 on reduced SHRs' BP were better than those of YSP-TN1 for one oral administration. For oral administration of 40 mg YSP-TN1 kg-1 SHR, the reduced MBP was 21.5 mmHg, which was comparable to 25.2 mmHg (the fourth hour) of 10 mg captopril kg-1 SHR oral administration. For oral administration of 40 mg PH-TN1 kg-1 SHR, the reduced MBP was 33.7 mmHg, comparable to 38.4 mmHg (the fourth hour) of 15 mg captopril kg-1 SHR. For long-term 25-day oral administration of 40 mg YSP-TN1 kg-1 SHR once a day, it was found that a feeding trial of YSP-TN1 effectively lowered SHRs' SBP, DBP and MBP. The greatest reduction in SHRs' blood pressure was reached on the ninth day, for the reduced SBP, 27.7 mmHg; for the reduced DBP, 28.3 mmHg; and for the reduced MBP, 27.5 mmHg.

AB - Yam storage protein (YSP) was purified from tubers of Dioscorea alata L. Tainong No. 1 (TN1) to homogeneity by DE-52 ion-exchange chromatography. The short-term (24 h) and long-term (25 days) antihypertensive effects of YSP-TN1 and its peptic hydrolyzates (PH-TN1) were measured in spontaneously hypertensive rats (SHRs). For 24-h antihypertensive measurements, SHRs (age 10 weeks, body weight from 240 to 250 g) were administered orally once (YSP-TN1 and PH-TN1, 40 mg kg-1 SHR) to measure the mean blood pressure (MBP), systolic blood pressure (SBP) and diastolic blood pressure (DBP). For a long-term antihypertensive measurement, SHRs (age 12 weeks, body weight from 250 to 270 g) were administered orally once a day for 25 days (YSP-TN1, 40 mg kg-1 SHR) to measure SBP, DBP and MBP. Captopril (10 or 15 mg kg-1 SHR) was used as a positive control. It was found that short-term administration of 40 mg kg-1 SHR of YSP-TN1l and PH-TN1 effectively lowered SHRs' MBP, SBP and DBP (For YSP-TN1, the lowest blood pressure was reached in the fourth hour and for PH-TN1 in the eighth hour). The lasting effects of PH-TN1 on reduced SHRs' BP were better than those of YSP-TN1 for one oral administration. For oral administration of 40 mg YSP-TN1 kg-1 SHR, the reduced MBP was 21.5 mmHg, which was comparable to 25.2 mmHg (the fourth hour) of 10 mg captopril kg-1 SHR oral administration. For oral administration of 40 mg PH-TN1 kg-1 SHR, the reduced MBP was 33.7 mmHg, comparable to 38.4 mmHg (the fourth hour) of 15 mg captopril kg-1 SHR. For long-term 25-day oral administration of 40 mg YSP-TN1 kg-1 SHR once a day, it was found that a feeding trial of YSP-TN1 effectively lowered SHRs' SBP, DBP and MBP. The greatest reduction in SHRs' blood pressure was reached on the ninth day, for the reduced SBP, 27.7 mmHg; for the reduced DBP, 28.3 mmHg; and for the reduced MBP, 27.5 mmHg.

KW - Antihypertension

KW - Diastolic blood pressure (DBP)

KW - Means of blood pressure (MBP)

KW - Peptic hydrolyzates

KW - Spontaneously hypertensive rat (SHR)

KW - Storage protein

KW - Systolic blood pressure (SBP)

KW - Yam

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U2 - 10.1002/jsfa.2530

DO - 10.1002/jsfa.2530

M3 - Article

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VL - 86

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JO - Journal of the Science of Food and Agriculture

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SN - 0022-5142

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