Abstract

Background/purpose: The aim of this study was to examine the effects of the time period between tooth extraction and the freezing procedure on pulp cells in frozen intact teeth of rats. Material and methods: In total, 120 incisors from 30 rats were extracted and kept in transportation solution for 0 hour, 12 hours, and 24 hours. The tested teeth were divided into 2 experimental groups, where the extracted incisors were first frozen in a magnetic-programmed freezer (PF) or a traditional -20°C freezer (TF). The tested teeth were then stored at -150°C for 7 days. Incisors extracted from the opposite side of the same rat were treated as the non-frozen control. After thawing, the pulp was extracted and dissected. Hematoxylin and eosin staining was performed to observe the cell distributions. Cell densities in the odontoblast region and cell-rich zone were calculated using an optical microscope.

Original languageEnglish
Pages (from-to)48-52
Number of pages5
JournalJournal of Dental Sciences
Volume6
Issue number1
DOIs
Publication statusPublished - Mar 2011

Keywords

  • Cell number
  • Cryopreservation
  • Magnetic-programmed freezer

ASJC Scopus subject areas

  • Dentistry(all)

Fingerprint Dive into the research topics of 'Effects of transportation time after extraction on the magnetic cryopreservation of pulp cells of rat dental pulp'. Together they form a unique fingerprint.

  • Cite this