Abstract
Background/purpose: The aim of this study was to examine the effects of the time period between tooth extraction and the freezing procedure on pulp cells in frozen intact teeth of rats. Material and methods: In total, 120 incisors from 30 rats were extracted and kept in transportation solution for 0 hour, 12 hours, and 24 hours. The tested teeth were divided into 2 experimental groups, where the extracted incisors were first frozen in a magnetic-programmed freezer (PF) or a traditional -20°C freezer (TF). The tested teeth were then stored at -150°C for 7 days. Incisors extracted from the opposite side of the same rat were treated as the non-frozen control. After thawing, the pulp was extracted and dissected. Hematoxylin and eosin staining was performed to observe the cell distributions. Cell densities in the odontoblast region and cell-rich zone were calculated using an optical microscope.
| Original language | English |
|---|---|
| Pages (from-to) | 48-52 |
| Number of pages | 5 |
| Journal | Journal of Dental Sciences |
| Volume | 6 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Mar 2011 |
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Keywords
- Cell number
- Cryopreservation
- Magnetic-programmed freezer
ASJC Scopus subject areas
- Dentistry(all)
Cite this
Effects of transportation time after extraction on the magnetic cryopreservation of pulp cells of rat dental pulp. / Huang, Mao-Suan; Chang, Wei-Jen; Huang, Haw-Ming; Lin, Yen Chuang; Huang, Yen-Hua; Yang, Jen-Chang; Lee, Sheng-Yang.
In: Journal of Dental Sciences, Vol. 6, No. 1, 03.2011, p. 48-52.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of transportation time after extraction on the magnetic cryopreservation of pulp cells of rat dental pulp
AU - Huang, Mao-Suan
AU - Chang, Wei-Jen
AU - Huang, Haw-Ming
AU - Lin, Yen Chuang
AU - Huang, Yen-Hua
AU - Yang, Jen-Chang
AU - Lee, Sheng-Yang
PY - 2011/3
Y1 - 2011/3
N2 - Background/purpose: The aim of this study was to examine the effects of the time period between tooth extraction and the freezing procedure on pulp cells in frozen intact teeth of rats. Material and methods: In total, 120 incisors from 30 rats were extracted and kept in transportation solution for 0 hour, 12 hours, and 24 hours. The tested teeth were divided into 2 experimental groups, where the extracted incisors were first frozen in a magnetic-programmed freezer (PF) or a traditional -20°C freezer (TF). The tested teeth were then stored at -150°C for 7 days. Incisors extracted from the opposite side of the same rat were treated as the non-frozen control. After thawing, the pulp was extracted and dissected. Hematoxylin and eosin staining was performed to observe the cell distributions. Cell densities in the odontoblast region and cell-rich zone were calculated using an optical microscope.
AB - Background/purpose: The aim of this study was to examine the effects of the time period between tooth extraction and the freezing procedure on pulp cells in frozen intact teeth of rats. Material and methods: In total, 120 incisors from 30 rats were extracted and kept in transportation solution for 0 hour, 12 hours, and 24 hours. The tested teeth were divided into 2 experimental groups, where the extracted incisors were first frozen in a magnetic-programmed freezer (PF) or a traditional -20°C freezer (TF). The tested teeth were then stored at -150°C for 7 days. Incisors extracted from the opposite side of the same rat were treated as the non-frozen control. After thawing, the pulp was extracted and dissected. Hematoxylin and eosin staining was performed to observe the cell distributions. Cell densities in the odontoblast region and cell-rich zone were calculated using an optical microscope.
KW - Cell number
KW - Cryopreservation
KW - Magnetic-programmed freezer
UR - http://www.scopus.com/inward/record.url?scp=84860417269&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860417269&partnerID=8YFLogxK
U2 - 10.1016/j.jds.2011.02.008
DO - 10.1016/j.jds.2011.02.008
M3 - Article
VL - 6
SP - 48
EP - 52
JO - Journal of Dental Sciences
JF - Journal of Dental Sciences
SN - 1991-7902
IS - 1
ER -