Abstract

Background/purpose: The aim of this study was to examine the effects of the time period between tooth extraction and the freezing procedure on pulp cells in frozen intact teeth of rats. Material and methods: In total, 120 incisors from 30 rats were extracted and kept in transportation solution for 0 hour, 12 hours, and 24 hours. The tested teeth were divided into 2 experimental groups, where the extracted incisors were first frozen in a magnetic-programmed freezer (PF) or a traditional -20°C freezer (TF). The tested teeth were then stored at -150°C for 7 days. Incisors extracted from the opposite side of the same rat were treated as the non-frozen control. After thawing, the pulp was extracted and dissected. Hematoxylin and eosin staining was performed to observe the cell distributions. Cell densities in the odontoblast region and cell-rich zone were calculated using an optical microscope.

Original languageEnglish
Pages (from-to)48-52
Number of pages5
JournalJournal of Dental Sciences
Volume6
Issue number1
DOIs
Publication statusPublished - Mar 2011

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Dental Pulp
Cryopreservation
Incisor
Tooth
Odontoblasts
Tooth Extraction
Hematoxylin
Eosine Yellowish-(YS)
Freezing
Cell Count
Staining and Labeling

Keywords

  • Cell number
  • Cryopreservation
  • Magnetic-programmed freezer

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

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title = "Effects of transportation time after extraction on the magnetic cryopreservation of pulp cells of rat dental pulp",
abstract = "Background/purpose: The aim of this study was to examine the effects of the time period between tooth extraction and the freezing procedure on pulp cells in frozen intact teeth of rats. Material and methods: In total, 120 incisors from 30 rats were extracted and kept in transportation solution for 0 hour, 12 hours, and 24 hours. The tested teeth were divided into 2 experimental groups, where the extracted incisors were first frozen in a magnetic-programmed freezer (PF) or a traditional -20°C freezer (TF). The tested teeth were then stored at -150°C for 7 days. Incisors extracted from the opposite side of the same rat were treated as the non-frozen control. After thawing, the pulp was extracted and dissected. Hematoxylin and eosin staining was performed to observe the cell distributions. Cell densities in the odontoblast region and cell-rich zone were calculated using an optical microscope.",
keywords = "Cell number, Cryopreservation, Magnetic-programmed freezer",
author = "Mao-Suan Huang and Wei-Jen Chang and Haw-Ming Huang and Lin, {Yen Chuang} and Yen-Hua Huang and Jen-Chang Yang and Sheng-Yang Lee",
year = "2011",
month = "3",
doi = "10.1016/j.jds.2011.02.008",
language = "English",
volume = "6",
pages = "48--52",
journal = "Journal of Dental Sciences",
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TY - JOUR

T1 - Effects of transportation time after extraction on the magnetic cryopreservation of pulp cells of rat dental pulp

AU - Huang, Mao-Suan

AU - Chang, Wei-Jen

AU - Huang, Haw-Ming

AU - Lin, Yen Chuang

AU - Huang, Yen-Hua

AU - Yang, Jen-Chang

AU - Lee, Sheng-Yang

PY - 2011/3

Y1 - 2011/3

N2 - Background/purpose: The aim of this study was to examine the effects of the time period between tooth extraction and the freezing procedure on pulp cells in frozen intact teeth of rats. Material and methods: In total, 120 incisors from 30 rats were extracted and kept in transportation solution for 0 hour, 12 hours, and 24 hours. The tested teeth were divided into 2 experimental groups, where the extracted incisors were first frozen in a magnetic-programmed freezer (PF) or a traditional -20°C freezer (TF). The tested teeth were then stored at -150°C for 7 days. Incisors extracted from the opposite side of the same rat were treated as the non-frozen control. After thawing, the pulp was extracted and dissected. Hematoxylin and eosin staining was performed to observe the cell distributions. Cell densities in the odontoblast region and cell-rich zone were calculated using an optical microscope.

AB - Background/purpose: The aim of this study was to examine the effects of the time period between tooth extraction and the freezing procedure on pulp cells in frozen intact teeth of rats. Material and methods: In total, 120 incisors from 30 rats were extracted and kept in transportation solution for 0 hour, 12 hours, and 24 hours. The tested teeth were divided into 2 experimental groups, where the extracted incisors were first frozen in a magnetic-programmed freezer (PF) or a traditional -20°C freezer (TF). The tested teeth were then stored at -150°C for 7 days. Incisors extracted from the opposite side of the same rat were treated as the non-frozen control. After thawing, the pulp was extracted and dissected. Hematoxylin and eosin staining was performed to observe the cell distributions. Cell densities in the odontoblast region and cell-rich zone were calculated using an optical microscope.

KW - Cell number

KW - Cryopreservation

KW - Magnetic-programmed freezer

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VL - 6

SP - 48

EP - 52

JO - Journal of Dental Sciences

JF - Journal of Dental Sciences

SN - 1991-7902

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