Effects of thrombopoietin on megakaryocyte colony formation from leukemic cells at diagnosis and from marrow cells after induction chemotherapy for acute leukemias

D. C. Liang, L. Y. Shih, M. C. Kuo, S. H. Chen, H. C. Liu, A. Shimosaka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have studied the effects of recombinant human thrombopoietin (TPO, mpl ligand) on the megakaryocyte colony formation from control human bone marrow cells, human leukemia cells at diagnosis, and human bone marrow cells after induction chemotherapy for acute leukemias. In the control human bone marrow cells from four adults and nine children who had localized malignancy and histologically normal-looking marrow, TPO alone effectively stimulated megakaryocyte colony formation, and interleukin-3 (IL-3) synergized this. In 17 patients (13 adults and four children) with acute myeloid leukemia (AML) at diagnosis, TPO stimulated leukemic colony formation in only one patient with FAB M7 subtype. In 11 children with acute lymphoblastic leukemia (ALL) at diagnosis, TPO did not enhance leukemic colony formation. After 17 courses of induction chemotherapy, nine for AML and eight for ALL, TPO stimulated megakaryocyte colony formation to a level of 51% of that in the control human bone marrow cells. This may suggest that the administration of TPO to patients with M7 subtype warrants caution, whereas it is probably safe to give TPO at any time to patients with ALL. The administration of TPO to patients with acute leukemias after induction chemotherapy could stimulate megakaryocytopoiesis.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalAnnals of Hematology
Volume77
Issue number1-2
DOIs
Publication statusPublished - Jul 1998
Externally publishedYes

Fingerprint

Thrombopoietin
Induction Chemotherapy
Megakaryocytes
Leukemia
Bone Marrow
Bone Marrow Cells
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Acute Myeloid Leukemia
Thrombopoiesis
Interleukin-3
Neoplasms

Keywords

  • Acute lymphoblastic leukemia
  • Acute myeloid leukemia
  • CFU-MK
  • Induction chemotherapy
  • Megakaryocyte colony
  • Thrombopoietin

ASJC Scopus subject areas

  • Hematology

Cite this

Effects of thrombopoietin on megakaryocyte colony formation from leukemic cells at diagnosis and from marrow cells after induction chemotherapy for acute leukemias. / Liang, D. C.; Shih, L. Y.; Kuo, M. C.; Chen, S. H.; Liu, H. C.; Shimosaka, A.

In: Annals of Hematology, Vol. 77, No. 1-2, 07.1998, p. 33-39.

Research output: Contribution to journalArticle

@article{db05117b05584f989104dc185a339020,
title = "Effects of thrombopoietin on megakaryocyte colony formation from leukemic cells at diagnosis and from marrow cells after induction chemotherapy for acute leukemias",
abstract = "We have studied the effects of recombinant human thrombopoietin (TPO, mpl ligand) on the megakaryocyte colony formation from control human bone marrow cells, human leukemia cells at diagnosis, and human bone marrow cells after induction chemotherapy for acute leukemias. In the control human bone marrow cells from four adults and nine children who had localized malignancy and histologically normal-looking marrow, TPO alone effectively stimulated megakaryocyte colony formation, and interleukin-3 (IL-3) synergized this. In 17 patients (13 adults and four children) with acute myeloid leukemia (AML) at diagnosis, TPO stimulated leukemic colony formation in only one patient with FAB M7 subtype. In 11 children with acute lymphoblastic leukemia (ALL) at diagnosis, TPO did not enhance leukemic colony formation. After 17 courses of induction chemotherapy, nine for AML and eight for ALL, TPO stimulated megakaryocyte colony formation to a level of 51{\%} of that in the control human bone marrow cells. This may suggest that the administration of TPO to patients with M7 subtype warrants caution, whereas it is probably safe to give TPO at any time to patients with ALL. The administration of TPO to patients with acute leukemias after induction chemotherapy could stimulate megakaryocytopoiesis.",
keywords = "Acute lymphoblastic leukemia, Acute myeloid leukemia, CFU-MK, Induction chemotherapy, Megakaryocyte colony, Thrombopoietin",
author = "Liang, {D. C.} and Shih, {L. Y.} and Kuo, {M. C.} and Chen, {S. H.} and Liu, {H. C.} and A. Shimosaka",
year = "1998",
month = "7",
doi = "10.1007/s002770050408",
language = "English",
volume = "77",
pages = "33--39",
journal = "Annals of Hematology",
issn = "0939-5555",
publisher = "Springer Verlag",
number = "1-2",

}

TY - JOUR

T1 - Effects of thrombopoietin on megakaryocyte colony formation from leukemic cells at diagnosis and from marrow cells after induction chemotherapy for acute leukemias

AU - Liang, D. C.

AU - Shih, L. Y.

AU - Kuo, M. C.

AU - Chen, S. H.

AU - Liu, H. C.

AU - Shimosaka, A.

PY - 1998/7

Y1 - 1998/7

N2 - We have studied the effects of recombinant human thrombopoietin (TPO, mpl ligand) on the megakaryocyte colony formation from control human bone marrow cells, human leukemia cells at diagnosis, and human bone marrow cells after induction chemotherapy for acute leukemias. In the control human bone marrow cells from four adults and nine children who had localized malignancy and histologically normal-looking marrow, TPO alone effectively stimulated megakaryocyte colony formation, and interleukin-3 (IL-3) synergized this. In 17 patients (13 adults and four children) with acute myeloid leukemia (AML) at diagnosis, TPO stimulated leukemic colony formation in only one patient with FAB M7 subtype. In 11 children with acute lymphoblastic leukemia (ALL) at diagnosis, TPO did not enhance leukemic colony formation. After 17 courses of induction chemotherapy, nine for AML and eight for ALL, TPO stimulated megakaryocyte colony formation to a level of 51% of that in the control human bone marrow cells. This may suggest that the administration of TPO to patients with M7 subtype warrants caution, whereas it is probably safe to give TPO at any time to patients with ALL. The administration of TPO to patients with acute leukemias after induction chemotherapy could stimulate megakaryocytopoiesis.

AB - We have studied the effects of recombinant human thrombopoietin (TPO, mpl ligand) on the megakaryocyte colony formation from control human bone marrow cells, human leukemia cells at diagnosis, and human bone marrow cells after induction chemotherapy for acute leukemias. In the control human bone marrow cells from four adults and nine children who had localized malignancy and histologically normal-looking marrow, TPO alone effectively stimulated megakaryocyte colony formation, and interleukin-3 (IL-3) synergized this. In 17 patients (13 adults and four children) with acute myeloid leukemia (AML) at diagnosis, TPO stimulated leukemic colony formation in only one patient with FAB M7 subtype. In 11 children with acute lymphoblastic leukemia (ALL) at diagnosis, TPO did not enhance leukemic colony formation. After 17 courses of induction chemotherapy, nine for AML and eight for ALL, TPO stimulated megakaryocyte colony formation to a level of 51% of that in the control human bone marrow cells. This may suggest that the administration of TPO to patients with M7 subtype warrants caution, whereas it is probably safe to give TPO at any time to patients with ALL. The administration of TPO to patients with acute leukemias after induction chemotherapy could stimulate megakaryocytopoiesis.

KW - Acute lymphoblastic leukemia

KW - Acute myeloid leukemia

KW - CFU-MK

KW - Induction chemotherapy

KW - Megakaryocyte colony

KW - Thrombopoietin

UR - http://www.scopus.com/inward/record.url?scp=0031694277&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031694277&partnerID=8YFLogxK

U2 - 10.1007/s002770050408

DO - 10.1007/s002770050408

M3 - Article

VL - 77

SP - 33

EP - 39

JO - Annals of Hematology

JF - Annals of Hematology

SN - 0939-5555

IS - 1-2

ER -