Effects of thermosensitive chitosan-gelatin based hydrogel containing glutathione on Cisd2-deficient chondrocytes under oxidative stress

Yung Hsin Cheng, Eddy Chavez, Kun Ling Tsai, Kai Chiang Yang, Wei Ting Kuo, Yi Ping Yang, Shih Hwa Chiou, Feng Huei Lin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Aging is considered as a primary risk factor in the development of osteoarthritis (OA) which associated with mitochondrial dysfunction and oxidative stress. CDGSH iron sulfur domain 2 (Cisd2) deficiency causes mitochondrial dysfunction and drive premature aging. In the present study, thermosensitive chitosan-gelatin based hydrogel containing glutathione was developed as injectable drug delivery system for administration by minimal invasive surgery for the treatment of OA. Cisd2 deficiency (Cisd2−/−) mouse induced pluripotent stem cells-derived chondrocytes were established and characterized. The results suggested that 100 μM of glutathione may be an optimal concentration to treat Cisd2−/− chondrocytes without cytotoxicity. The developed hydrogel showed sustained release profile of the glutathione and could decrease the reactive oxygen species level. Post-treatment of glutathione-loaded hydrogel could rescue Cisd2−/− chondrocytes from oxidative damage via increasing catalase activity, down-regulation of inflammation, and decreasing apoptosis. These results suggest that thermosensitive glutathione-loaded hydrogel may be a potential antioxidant therapeutic strategy for treating Cisd2−/− chondrocytes in the near future.

Original languageEnglish
Pages (from-to)17-27
Number of pages11
JournalCarbohydrate Polymers
Volume173
DOIs
Publication statusPublished - Oct 1 2017

Keywords

  • CDGSH iron sulfur domain 2
  • Glutathione
  • Hydrogel
  • Oxidative stress

ASJC Scopus subject areas

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

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