Effects of temple particles on inflammation and endothelial cell response

Lian Yu Lin, Hui Yi Lin, Hua Wei Chen, Te Li Su, Li Chu Huang, Kai Jen Chuang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To pray in temples is a regular activity in Buddhism and Taoism societies, yet few studies investigated the effects of particles from incense-burning in temples. The objectives of this study are to examine particle size and polycyclic aromatic hydrocarbon (PAH) effects of particles on coronary artery endothelial cell. We used two micro-orifice uniform deposit impactors to collect 11 sets of particles at a Chinese temple in Yi-Lan, Taiwan. 16 PAHs were determined by a high-resolution gas chomatograph/high-resolution mass spectrometer. Human coronary artery endothelial cells were exposed to particle extracts in three size ranges: PM0.1 (diameters less than 0.1μm), PM1.0-0.1 (diameters between 1.0 and 0.1μm), and PM10-1.0 (diameters between 10 and 1.0μm) at 50μg/mL for 4h, and interleukin-6 (IL-6), endothelin-1 (ET-1), and nitric oxide (NO) concentrations in the medium were measured. We found that PM1.0-0.1 stimulation resulted in significantly higher IL-6 and ET-1 production than PM0.1 and PM10-1.0. PM1.0-0.1 also significantly reduced HCAEA cells to synthesize NO. Naphthalene, acenaphthylene, acenaphthene and anthracene of PM1.0-0.1 were highly correlated with NO reduction. This study found that size and composition of temple particles were both important factors in inducing cytokine production and reducing NO formation in human coronary artery endothelial cell cultures.

Original languageEnglish
Pages (from-to)68-72
Number of pages5
JournalScience of the Total Environment
Volume414
DOIs
Publication statusPublished - Jan 1 2012
Externally publishedYes

Fingerprint

Endothelial cells
Nitric oxide
nitric oxide
Nitric Oxide
Endothelin-1
Polycyclic aromatic hydrocarbons
Interleukin-6
PAH
Anthracene
Polycyclic Aromatic Hydrocarbons
Mass spectrometers
Naphthalene
Orifices
Cell culture
naphthalene
Deposits
Gases
Particle size
range size
Cytokines

Keywords

  • Human coronary artery endothelial cell
  • Polycyclic aromatic hydrocarbons
  • Temple particles

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution
  • Waste Management and Disposal
  • Environmental Engineering

Cite this

Effects of temple particles on inflammation and endothelial cell response. / Lin, Lian Yu; Lin, Hui Yi; Chen, Hua Wei; Su, Te Li; Huang, Li Chu; Chuang, Kai Jen.

In: Science of the Total Environment, Vol. 414, 01.01.2012, p. 68-72.

Research output: Contribution to journalArticle

Lin, Lian Yu ; Lin, Hui Yi ; Chen, Hua Wei ; Su, Te Li ; Huang, Li Chu ; Chuang, Kai Jen. / Effects of temple particles on inflammation and endothelial cell response. In: Science of the Total Environment. 2012 ; Vol. 414. pp. 68-72.
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