Effects of Supraphysiological Testosterone Treatment and Orchiectomy on Ischemia/Reperfusion-Induced Bladder Dysfunction in Male Rabbits

Shu Mien Chuang, Keh Min Liu, Yung Chin Lee, Rong Jyh Lin, Chao Yuan Chang, Wen Jen Wu, Wei Chiao Chang, Robert M. Levin, Yung Shun Juan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Introduction: The roles of testosterone and orchiectomy on male bladder subjected to ischemic/reperfusion (I/R) injuries received little attention. To fill this gap, the present study intended to examine testosterone and orchiectomy effects on male rabbits subjected to I/R damages. Aim: To elucidate the effects of testosterone and orchiectomy on contractile response, bladder morphology, interstitial fibrosis, and oxidative stress in male rabbit bladder subjected to I/R surgery. Methods: Male New Zealand rabbits were distributed into five groups as follows: Group 1 received sham surgical procedure. In group 2, I/R surgery was performed. In group 3, testosterone (100μg/kg/day) was intramuscularly injected prior to I/R surgery. In group 4, orchiectomy was performed prior to I/R surgery. In group 5, orchiectomy was performed with subsequent testosterone administration, followed by I/R surgery. All the rabbits were euthanized 7 days after I/R. Comparative studies were analyzed to elucidate the effects of testosterone and orchiectomy on bladder dysfunction subjected to I/R injuries. Main Outcome Measures: Bladder contractile function was evaluated. Masson's trichrome staining and immunohistochemical studies were performed to evaluate bladder morphology and intramural nerve terminals. Western blotting was examined to investigate the expressions of fibrosis and oxidative stress markers. Results: I/R surgery significantly decreased bladder contractility in response to various stimulations with and without testosterone treatment. I/R damages decreased bladder nerve density with and without testosterone. The expressions of fibrosis and oxidative stress-related proteins were increased by I/R injuries with or without testosterone treatment. Testosterone depletion significantly decreased the expressions of transforming growth factor-β and fibronectin expressions after I/R injury. Supraphysiological testosterone treatment after orchiectomy greatly increased the expressions of these fibrosis proteins; however, orchiectomy alone ameliorated I/R injuries. Conclusions: Testosterone treatment or orchiectomy affected I/R-induced bladder damages in male rabbits. Orchiectomy decreased the level of fibrosis and oxidative stress markers and increased neurofilament densities. Supraphysiological exogenous testosterone administration after orchiectomy further exacerbated such detrimental effects of I/R.

Original languageEnglish
Pages (from-to)1278-1290
Number of pages13
JournalJournal of Sexual Medicine
Volume10
Issue number5
DOIs
Publication statusPublished - May 2013

Fingerprint

Orchiectomy
Reperfusion
Testosterone
Urinary Bladder
Ischemia
Rabbits
Reperfusion Injury
Fibrosis
Oxidative Stress
Intermediate Filaments
Transforming Growth Factors
Heat-Shock Proteins
Fibronectins

Keywords

  • Bladder
  • Ischemia/Reperfusion
  • Orchiectomy
  • Testosterone

ASJC Scopus subject areas

  • Urology
  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Effects of Supraphysiological Testosterone Treatment and Orchiectomy on Ischemia/Reperfusion-Induced Bladder Dysfunction in Male Rabbits. / Chuang, Shu Mien; Liu, Keh Min; Lee, Yung Chin; Lin, Rong Jyh; Chang, Chao Yuan; Wu, Wen Jen; Chang, Wei Chiao; Levin, Robert M.; Juan, Yung Shun.

In: Journal of Sexual Medicine, Vol. 10, No. 5, 05.2013, p. 1278-1290.

Research output: Contribution to journalArticle

Chuang, Shu Mien ; Liu, Keh Min ; Lee, Yung Chin ; Lin, Rong Jyh ; Chang, Chao Yuan ; Wu, Wen Jen ; Chang, Wei Chiao ; Levin, Robert M. ; Juan, Yung Shun. / Effects of Supraphysiological Testosterone Treatment and Orchiectomy on Ischemia/Reperfusion-Induced Bladder Dysfunction in Male Rabbits. In: Journal of Sexual Medicine. 2013 ; Vol. 10, No. 5. pp. 1278-1290.
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N2 - Introduction: The roles of testosterone and orchiectomy on male bladder subjected to ischemic/reperfusion (I/R) injuries received little attention. To fill this gap, the present study intended to examine testosterone and orchiectomy effects on male rabbits subjected to I/R damages. Aim: To elucidate the effects of testosterone and orchiectomy on contractile response, bladder morphology, interstitial fibrosis, and oxidative stress in male rabbit bladder subjected to I/R surgery. Methods: Male New Zealand rabbits were distributed into five groups as follows: Group 1 received sham surgical procedure. In group 2, I/R surgery was performed. In group 3, testosterone (100μg/kg/day) was intramuscularly injected prior to I/R surgery. In group 4, orchiectomy was performed prior to I/R surgery. In group 5, orchiectomy was performed with subsequent testosterone administration, followed by I/R surgery. All the rabbits were euthanized 7 days after I/R. Comparative studies were analyzed to elucidate the effects of testosterone and orchiectomy on bladder dysfunction subjected to I/R injuries. Main Outcome Measures: Bladder contractile function was evaluated. Masson's trichrome staining and immunohistochemical studies were performed to evaluate bladder morphology and intramural nerve terminals. Western blotting was examined to investigate the expressions of fibrosis and oxidative stress markers. Results: I/R surgery significantly decreased bladder contractility in response to various stimulations with and without testosterone treatment. I/R damages decreased bladder nerve density with and without testosterone. The expressions of fibrosis and oxidative stress-related proteins were increased by I/R injuries with or without testosterone treatment. Testosterone depletion significantly decreased the expressions of transforming growth factor-β and fibronectin expressions after I/R injury. Supraphysiological testosterone treatment after orchiectomy greatly increased the expressions of these fibrosis proteins; however, orchiectomy alone ameliorated I/R injuries. Conclusions: Testosterone treatment or orchiectomy affected I/R-induced bladder damages in male rabbits. Orchiectomy decreased the level of fibrosis and oxidative stress markers and increased neurofilament densities. Supraphysiological exogenous testosterone administration after orchiectomy further exacerbated such detrimental effects of I/R.

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