Effects of Shock Waves on Tenocyte Proliferation and Extracellular Matrix Metabolism

Yuan Hung Chao, Yang Hwei Tsuang, Jui Sheng Sun, Li Ting Chen, Yueh Feng Chiang, Chien C. Wang, Ming Hong Chen

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The shock wave is an effective noninvasive modality for resolving various tendon pathologies. However, scientific rationale and mechanism of shock wave therapy remains limited. This study aims to investigate the effects of shock waves and their biochemical mechanisms on tenocyte proliferation and collagen synthesis. Tenocytes harvested from Achilles tendons of Sprague-Dawley rats were used in this study. Cell viability was assayed by trypan blue exclusion methods. The colorimetric assay was determined to evaluate the mitochondria activity of the tenocytes after shock wave exposure. Synthesis of collagen, nitric oxide (NO) and transforming growth factor-β1 (TGF-β1) were determined and their gene expression was also studied. The results showed that there was a dose-dependent impairment of cell viability observed in 0.36 mJ/mm2 and 0.68 mJ/mm2 stimulation. In the proliferation assay, low energy level with low impulses (0.36 mJ/mm2 with 50 and 100 impulses) showed positive stimulatory effects, whereas the high energy level with high impulses (0.68 mJ/mm2 with 250 and 500 impulses) had significant inhibitory effects. At 0.36 mJ/mm2, 100 impulse shock waves treatment, up-regulation of proliferating cell nuclear antigen (PCNA) (at 6 and 24 h) and collagen type I, collagen type III and TGF-β1 gene expression (at 24 h) were observed; these were followed by the increases in NO production (at 24 h), TGF-β1 release (at 48 and 96 h) and collagen synthesis (at the 7th day). This study revealed that shock waves can stimulate tenocyte proliferation and collagen synthesis. The associated tenocyte proliferation is mediated by early up-regulation of PCNA and TGF-β1 gene expression, endogenous NO release and synthesis and TGF-β1 protein and then collagen synthesis. (E-mail: jssun@ym.edu.tw).

Original languageEnglish
Pages (from-to)841-852
Number of pages12
JournalUltrasound in Medicine and Biology
Volume34
Issue number5
DOIs
Publication statusPublished - May 2008
Externally publishedYes

Fingerprint

Transforming Growth Factors
metabolism
collagens
Extracellular Matrix
shock waves
Collagen
matrices
impulses
Nitric Oxide
gene expression
nitric oxide
synthesis
Proliferating Cell Nuclear Antigen
Gene Expression
tendons
Cell Survival
Up-Regulation
antigens
viability
Convulsive Therapy

Keywords

  • Collagen
  • Nitric oxide
  • Proliferation
  • Shock waves
  • Tenocytes

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Effects of Shock Waves on Tenocyte Proliferation and Extracellular Matrix Metabolism. / Chao, Yuan Hung; Tsuang, Yang Hwei; Sun, Jui Sheng; Chen, Li Ting; Chiang, Yueh Feng; Wang, Chien C.; Chen, Ming Hong.

In: Ultrasound in Medicine and Biology, Vol. 34, No. 5, 05.2008, p. 841-852.

Research output: Contribution to journalArticle

Chao, Yuan Hung ; Tsuang, Yang Hwei ; Sun, Jui Sheng ; Chen, Li Ting ; Chiang, Yueh Feng ; Wang, Chien C. ; Chen, Ming Hong. / Effects of Shock Waves on Tenocyte Proliferation and Extracellular Matrix Metabolism. In: Ultrasound in Medicine and Biology. 2008 ; Vol. 34, No. 5. pp. 841-852.
@article{c6ffab9fcb7e45f2a341c85d8fe9fa83,
title = "Effects of Shock Waves on Tenocyte Proliferation and Extracellular Matrix Metabolism",
abstract = "The shock wave is an effective noninvasive modality for resolving various tendon pathologies. However, scientific rationale and mechanism of shock wave therapy remains limited. This study aims to investigate the effects of shock waves and their biochemical mechanisms on tenocyte proliferation and collagen synthesis. Tenocytes harvested from Achilles tendons of Sprague-Dawley rats were used in this study. Cell viability was assayed by trypan blue exclusion methods. The colorimetric assay was determined to evaluate the mitochondria activity of the tenocytes after shock wave exposure. Synthesis of collagen, nitric oxide (NO) and transforming growth factor-β1 (TGF-β1) were determined and their gene expression was also studied. The results showed that there was a dose-dependent impairment of cell viability observed in 0.36 mJ/mm2 and 0.68 mJ/mm2 stimulation. In the proliferation assay, low energy level with low impulses (0.36 mJ/mm2 with 50 and 100 impulses) showed positive stimulatory effects, whereas the high energy level with high impulses (0.68 mJ/mm2 with 250 and 500 impulses) had significant inhibitory effects. At 0.36 mJ/mm2, 100 impulse shock waves treatment, up-regulation of proliferating cell nuclear antigen (PCNA) (at 6 and 24 h) and collagen type I, collagen type III and TGF-β1 gene expression (at 24 h) were observed; these were followed by the increases in NO production (at 24 h), TGF-β1 release (at 48 and 96 h) and collagen synthesis (at the 7th day). This study revealed that shock waves can stimulate tenocyte proliferation and collagen synthesis. The associated tenocyte proliferation is mediated by early up-regulation of PCNA and TGF-β1 gene expression, endogenous NO release and synthesis and TGF-β1 protein and then collagen synthesis. (E-mail: jssun@ym.edu.tw).",
keywords = "Collagen, Nitric oxide, Proliferation, Shock waves, Tenocytes",
author = "Chao, {Yuan Hung} and Tsuang, {Yang Hwei} and Sun, {Jui Sheng} and Chen, {Li Ting} and Chiang, {Yueh Feng} and Wang, {Chien C.} and Chen, {Ming Hong}",
year = "2008",
month = "5",
doi = "10.1016/j.ultrasmedbio.2007.11.002",
language = "English",
volume = "34",
pages = "841--852",
journal = "Ultrasound in Medicine and Biology",
issn = "0301-5629",
publisher = "Elsevier USA",
number = "5",

}

TY - JOUR

T1 - Effects of Shock Waves on Tenocyte Proliferation and Extracellular Matrix Metabolism

AU - Chao, Yuan Hung

AU - Tsuang, Yang Hwei

AU - Sun, Jui Sheng

AU - Chen, Li Ting

AU - Chiang, Yueh Feng

AU - Wang, Chien C.

AU - Chen, Ming Hong

PY - 2008/5

Y1 - 2008/5

N2 - The shock wave is an effective noninvasive modality for resolving various tendon pathologies. However, scientific rationale and mechanism of shock wave therapy remains limited. This study aims to investigate the effects of shock waves and their biochemical mechanisms on tenocyte proliferation and collagen synthesis. Tenocytes harvested from Achilles tendons of Sprague-Dawley rats were used in this study. Cell viability was assayed by trypan blue exclusion methods. The colorimetric assay was determined to evaluate the mitochondria activity of the tenocytes after shock wave exposure. Synthesis of collagen, nitric oxide (NO) and transforming growth factor-β1 (TGF-β1) were determined and their gene expression was also studied. The results showed that there was a dose-dependent impairment of cell viability observed in 0.36 mJ/mm2 and 0.68 mJ/mm2 stimulation. In the proliferation assay, low energy level with low impulses (0.36 mJ/mm2 with 50 and 100 impulses) showed positive stimulatory effects, whereas the high energy level with high impulses (0.68 mJ/mm2 with 250 and 500 impulses) had significant inhibitory effects. At 0.36 mJ/mm2, 100 impulse shock waves treatment, up-regulation of proliferating cell nuclear antigen (PCNA) (at 6 and 24 h) and collagen type I, collagen type III and TGF-β1 gene expression (at 24 h) were observed; these were followed by the increases in NO production (at 24 h), TGF-β1 release (at 48 and 96 h) and collagen synthesis (at the 7th day). This study revealed that shock waves can stimulate tenocyte proliferation and collagen synthesis. The associated tenocyte proliferation is mediated by early up-regulation of PCNA and TGF-β1 gene expression, endogenous NO release and synthesis and TGF-β1 protein and then collagen synthesis. (E-mail: jssun@ym.edu.tw).

AB - The shock wave is an effective noninvasive modality for resolving various tendon pathologies. However, scientific rationale and mechanism of shock wave therapy remains limited. This study aims to investigate the effects of shock waves and their biochemical mechanisms on tenocyte proliferation and collagen synthesis. Tenocytes harvested from Achilles tendons of Sprague-Dawley rats were used in this study. Cell viability was assayed by trypan blue exclusion methods. The colorimetric assay was determined to evaluate the mitochondria activity of the tenocytes after shock wave exposure. Synthesis of collagen, nitric oxide (NO) and transforming growth factor-β1 (TGF-β1) were determined and their gene expression was also studied. The results showed that there was a dose-dependent impairment of cell viability observed in 0.36 mJ/mm2 and 0.68 mJ/mm2 stimulation. In the proliferation assay, low energy level with low impulses (0.36 mJ/mm2 with 50 and 100 impulses) showed positive stimulatory effects, whereas the high energy level with high impulses (0.68 mJ/mm2 with 250 and 500 impulses) had significant inhibitory effects. At 0.36 mJ/mm2, 100 impulse shock waves treatment, up-regulation of proliferating cell nuclear antigen (PCNA) (at 6 and 24 h) and collagen type I, collagen type III and TGF-β1 gene expression (at 24 h) were observed; these were followed by the increases in NO production (at 24 h), TGF-β1 release (at 48 and 96 h) and collagen synthesis (at the 7th day). This study revealed that shock waves can stimulate tenocyte proliferation and collagen synthesis. The associated tenocyte proliferation is mediated by early up-regulation of PCNA and TGF-β1 gene expression, endogenous NO release and synthesis and TGF-β1 protein and then collagen synthesis. (E-mail: jssun@ym.edu.tw).

KW - Collagen

KW - Nitric oxide

KW - Proliferation

KW - Shock waves

KW - Tenocytes

UR - http://www.scopus.com/inward/record.url?scp=42949131570&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42949131570&partnerID=8YFLogxK

U2 - 10.1016/j.ultrasmedbio.2007.11.002

DO - 10.1016/j.ultrasmedbio.2007.11.002

M3 - Article

C2 - 18222032

AN - SCOPUS:42949131570

VL - 34

SP - 841

EP - 852

JO - Ultrasound in Medicine and Biology

JF - Ultrasound in Medicine and Biology

SN - 0301-5629

IS - 5

ER -