Effects of serotonin on acid secretion in isolated rat stomach: The role of 5-HT3 receptors

Yung Chih Lai, Yih Ho, Kai Han Huang, Li-Hsueh Tsai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Serotonin (5-hydroxytryptamin; 5-HT) content has been measured using high-performance liquid chromatography with electrochemical detection (HPLC-ECD). The distributions of 5-HT-containing cells and 5-HT3 receptors have been determined with specific antibodies against 5-HT and 5-HT3 receptors, respectively. The effect of serotonin on acid secretion has been studied using an isolated rat stomach model. It has been shown that 5-HT concentrations in the fundus, mucosal layers of the corpus, remaining layer of the corpus and antrum are approximately 152, 498, 1494 and 972 nmol/mg protein, respectively. The distribution of 5-HT-containing cells is concentrated in the enteric plexus and enterochromaffin (EC) cells in the deep mucosal layer. Immunoreactivity to 5-HT3 receptors is localized in numerous neurons of the myenteric and submucosal plexus and concentrated in the neuronal plasma membrane, submucosa, endocrine cells and lamina propria. In the present study, the effect of 5-HT on gastric acid secretion was investigated on an everted preparation of isolated rat stomach. 5-HT at 1-100 μM reduced acid secretion stimulated by oxotremorine while 10 μM 5-HT did not modify the basal secretion of gastric acid. We further showed that 10 μM 5-HT reduced acid secretion and pepsin output stimulated by oxotremorine, histamine and pentagastrin; among the 5-HT receptors agonists tested, 2-methyl-5-HT (1-10 μM), a 5-HT3 receptor agonist, inhibited oxotremorine-, histamine- and pentagastrin-stimulated acid secretions, and this inhibitory effect was blocked by 1 μM MDL 72222, a specific 5-HT3 receptor antagonist. These results suggest that 5-HT is released from serotoninergic neurons, their processes and EC cells. The effect of 5-HT mediated by 5-HT3 receptors involves distinct neuronal and non-neuronal pathways which modulate gastric acid secretion.

Original languageEnglish
Pages (from-to)395-405
Number of pages11
JournalChinese Journal of Physiology
Volume52
Issue numberSUPPL. 5
DOIs
Publication statusPublished - 2009

Fingerprint

Receptors, Serotonin, 5-HT3
Serotonin
Stomach
Acids
Oxotremorine
Gastric Acid
Enterochromaffin Cells
Pentagastrin
Histamine
Serotonin 5-HT3 Receptor Agonists
Submucous Plexus
Serotonin 5-HT3 Receptor Antagonists
Serotonergic Neurons
Myenteric Plexus
Serotonin Receptor Agonists
Endocrine Cells
Serotonin Receptors
Pepsin A

Keywords

  • 5-HT
  • 5-HT receptors
  • Acid secretion
  • Enteric plexus
  • Isolated stomach

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Effects of serotonin on acid secretion in isolated rat stomach : The role of 5-HT3 receptors. / Lai, Yung Chih; Ho, Yih; Huang, Kai Han; Tsai, Li-Hsueh.

In: Chinese Journal of Physiology, Vol. 52, No. SUPPL. 5, 2009, p. 395-405.

Research output: Contribution to journalArticle

Lai, Yung Chih ; Ho, Yih ; Huang, Kai Han ; Tsai, Li-Hsueh. / Effects of serotonin on acid secretion in isolated rat stomach : The role of 5-HT3 receptors. In: Chinese Journal of Physiology. 2009 ; Vol. 52, No. SUPPL. 5. pp. 395-405.
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