Effects of selective unilateral dorsal root(s) rhizotomy on micturition reflex in anesthetized rats

Ming Cheng Tsai, Chih Yung Tang, Ya Ting Chang, Chen Li Cheng, Jiuan Miaw Liao, Shin Da Lee, Shwu Fen Pan, Mei Jung Chen, Pei Chen Huang, Tzer Bin Lin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: To clarify the contributions of sensory inputs and glutamate transmissions to the spinal micturition reflex. Materials and Methods: Cystometrogram and external urethral sphincter electromyogram activities evaluated the L6 and/or S1 levels. Results: Changes in intravesicular pressure (IVP) in response to saline infusion (0.1 ml/min) were found after unilateral dorsal root rhizotomy at the L6 level, which showed significant increases in threshold pressure (rhizotomized vs. control: 14.25 ± 0.82 vs. 8.40 ± 0.69 cmH2O, P <0.01, n = 28), post-voiding pressure (7.66 ± 0.56 vs. 5.42 ± 0.52 cmH2O, P <0.01, n = 28), holding duration (135.06 ± 23.6 vs. 77.73 ± 13.56 sec, P <0.05, n = 28), and inter-contraction interval (140.62 ± 23.29 vs. 82.40 ± 13.57 sec, P <0.05, n = 28). Several (mean = 2.32 ± 1.31 vs. 0.12 ± 0.21, P <0.01, n = 28, P <0.01, n = 28) non-voiding contractions with gradual increase in IVP were found ahead of voiding contraction after rhizotomy. An additional dorsal root rhizotomy at the ipsilateral S1 level caused further increases in urodynamic parameters (threshold pressure, 18.18 ± 1.67 cmH2O, P <0.01; post-voiding pressure 8.07 ± 0.96 cmH2O, P <0.01; holding duration, 211.44 ± 42.54 sec, P <0.01; inter-contraction interval, 264.2 ± 59.99 sec, P <0.05; non-voiding contractions, 4.41 ± 2.12, P <0.01, n = 7). Intrathecal glutamate (100 μM, 10 μl) ameliorated all the pathological conditions induced by unilateral dorsal root rhizotomy at the L6 level in a dose dependent manner (ED50 = 1.25 ± 10-5). Intrathecal CNQX (6-cyano-7-nitroquinoxaline-2,3- dione; 100 μM, 10 μl) and APV (D-2-amino-5-phosphonovaleric acid; 100 μM, 10 μl) injections after rhizotomy at the L6 level induced disturbances similar to that caused by an additional rhizotomy at ipsilateral S1 level. Wherease, glutamate (100 μM, 10 μl) reversed the disturbances caused by CNQX but showed no effect on that by APV. Conclusions: Acute partial sensory deprivation caused acute impaired micturition reflex in rat models. In addition, glutamatergic NMDA and AMPA receptors are important for mediating these impairments in micturition reflex.

Original languageEnglish
Pages (from-to)820-827
Number of pages8
JournalNeurourology and Urodynamics
Volume25
Issue number7
DOIs
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Rhizotomy
Urination
Spinal Nerve Roots
Reflex
6-Cyano-7-nitroquinoxaline-2,3-dione
Pressure
Glutamic Acid
Sensory Deprivation
2-Amino-5-phosphonovalerate
AMPA Receptors
Urodynamics
Electromyography
Urethra
N-Methyl-D-Aspartate Receptors
Injections

Keywords

  • AMPA
  • Glutamate
  • NMDA
  • Sensory
  • Voiding

ASJC Scopus subject areas

  • Clinical Neurology
  • Nephrology
  • Urology

Cite this

Effects of selective unilateral dorsal root(s) rhizotomy on micturition reflex in anesthetized rats. / Tsai, Ming Cheng; Tang, Chih Yung; Chang, Ya Ting; Cheng, Chen Li; Liao, Jiuan Miaw; Lee, Shin Da; Pan, Shwu Fen; Chen, Mei Jung; Huang, Pei Chen; Lin, Tzer Bin.

In: Neurourology and Urodynamics, Vol. 25, No. 7, 2006, p. 820-827.

Research output: Contribution to journalArticle

Tsai, MC, Tang, CY, Chang, YT, Cheng, CL, Liao, JM, Lee, SD, Pan, SF, Chen, MJ, Huang, PC & Lin, TB 2006, 'Effects of selective unilateral dorsal root(s) rhizotomy on micturition reflex in anesthetized rats', Neurourology and Urodynamics, vol. 25, no. 7, pp. 820-827. https://doi.org/10.1002/nau.20211
Tsai, Ming Cheng ; Tang, Chih Yung ; Chang, Ya Ting ; Cheng, Chen Li ; Liao, Jiuan Miaw ; Lee, Shin Da ; Pan, Shwu Fen ; Chen, Mei Jung ; Huang, Pei Chen ; Lin, Tzer Bin. / Effects of selective unilateral dorsal root(s) rhizotomy on micturition reflex in anesthetized rats. In: Neurourology and Urodynamics. 2006 ; Vol. 25, No. 7. pp. 820-827.
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abstract = "Purpose: To clarify the contributions of sensory inputs and glutamate transmissions to the spinal micturition reflex. Materials and Methods: Cystometrogram and external urethral sphincter electromyogram activities evaluated the L6 and/or S1 levels. Results: Changes in intravesicular pressure (IVP) in response to saline infusion (0.1 ml/min) were found after unilateral dorsal root rhizotomy at the L6 level, which showed significant increases in threshold pressure (rhizotomized vs. control: 14.25 ± 0.82 vs. 8.40 ± 0.69 cmH2O, P <0.01, n = 28), post-voiding pressure (7.66 ± 0.56 vs. 5.42 ± 0.52 cmH2O, P <0.01, n = 28), holding duration (135.06 ± 23.6 vs. 77.73 ± 13.56 sec, P <0.05, n = 28), and inter-contraction interval (140.62 ± 23.29 vs. 82.40 ± 13.57 sec, P <0.05, n = 28). Several (mean = 2.32 ± 1.31 vs. 0.12 ± 0.21, P <0.01, n = 28, P <0.01, n = 28) non-voiding contractions with gradual increase in IVP were found ahead of voiding contraction after rhizotomy. An additional dorsal root rhizotomy at the ipsilateral S1 level caused further increases in urodynamic parameters (threshold pressure, 18.18 ± 1.67 cmH2O, P <0.01; post-voiding pressure 8.07 ± 0.96 cmH2O, P <0.01; holding duration, 211.44 ± 42.54 sec, P <0.01; inter-contraction interval, 264.2 ± 59.99 sec, P <0.05; non-voiding contractions, 4.41 ± 2.12, P <0.01, n = 7). Intrathecal glutamate (100 μM, 10 μl) ameliorated all the pathological conditions induced by unilateral dorsal root rhizotomy at the L6 level in a dose dependent manner (ED50 = 1.25 ± 10-5). Intrathecal CNQX (6-cyano-7-nitroquinoxaline-2,3- dione; 100 μM, 10 μl) and APV (D-2-amino-5-phosphonovaleric acid; 100 μM, 10 μl) injections after rhizotomy at the L6 level induced disturbances similar to that caused by an additional rhizotomy at ipsilateral S1 level. Wherease, glutamate (100 μM, 10 μl) reversed the disturbances caused by CNQX but showed no effect on that by APV. Conclusions: Acute partial sensory deprivation caused acute impaired micturition reflex in rat models. In addition, glutamatergic NMDA and AMPA receptors are important for mediating these impairments in micturition reflex.",
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T1 - Effects of selective unilateral dorsal root(s) rhizotomy on micturition reflex in anesthetized rats

AU - Tsai, Ming Cheng

AU - Tang, Chih Yung

AU - Chang, Ya Ting

AU - Cheng, Chen Li

AU - Liao, Jiuan Miaw

AU - Lee, Shin Da

AU - Pan, Shwu Fen

AU - Chen, Mei Jung

AU - Huang, Pei Chen

AU - Lin, Tzer Bin

PY - 2006

Y1 - 2006

N2 - Purpose: To clarify the contributions of sensory inputs and glutamate transmissions to the spinal micturition reflex. Materials and Methods: Cystometrogram and external urethral sphincter electromyogram activities evaluated the L6 and/or S1 levels. Results: Changes in intravesicular pressure (IVP) in response to saline infusion (0.1 ml/min) were found after unilateral dorsal root rhizotomy at the L6 level, which showed significant increases in threshold pressure (rhizotomized vs. control: 14.25 ± 0.82 vs. 8.40 ± 0.69 cmH2O, P <0.01, n = 28), post-voiding pressure (7.66 ± 0.56 vs. 5.42 ± 0.52 cmH2O, P <0.01, n = 28), holding duration (135.06 ± 23.6 vs. 77.73 ± 13.56 sec, P <0.05, n = 28), and inter-contraction interval (140.62 ± 23.29 vs. 82.40 ± 13.57 sec, P <0.05, n = 28). Several (mean = 2.32 ± 1.31 vs. 0.12 ± 0.21, P <0.01, n = 28, P <0.01, n = 28) non-voiding contractions with gradual increase in IVP were found ahead of voiding contraction after rhizotomy. An additional dorsal root rhizotomy at the ipsilateral S1 level caused further increases in urodynamic parameters (threshold pressure, 18.18 ± 1.67 cmH2O, P <0.01; post-voiding pressure 8.07 ± 0.96 cmH2O, P <0.01; holding duration, 211.44 ± 42.54 sec, P <0.01; inter-contraction interval, 264.2 ± 59.99 sec, P <0.05; non-voiding contractions, 4.41 ± 2.12, P <0.01, n = 7). Intrathecal glutamate (100 μM, 10 μl) ameliorated all the pathological conditions induced by unilateral dorsal root rhizotomy at the L6 level in a dose dependent manner (ED50 = 1.25 ± 10-5). Intrathecal CNQX (6-cyano-7-nitroquinoxaline-2,3- dione; 100 μM, 10 μl) and APV (D-2-amino-5-phosphonovaleric acid; 100 μM, 10 μl) injections after rhizotomy at the L6 level induced disturbances similar to that caused by an additional rhizotomy at ipsilateral S1 level. Wherease, glutamate (100 μM, 10 μl) reversed the disturbances caused by CNQX but showed no effect on that by APV. Conclusions: Acute partial sensory deprivation caused acute impaired micturition reflex in rat models. In addition, glutamatergic NMDA and AMPA receptors are important for mediating these impairments in micturition reflex.

AB - Purpose: To clarify the contributions of sensory inputs and glutamate transmissions to the spinal micturition reflex. Materials and Methods: Cystometrogram and external urethral sphincter electromyogram activities evaluated the L6 and/or S1 levels. Results: Changes in intravesicular pressure (IVP) in response to saline infusion (0.1 ml/min) were found after unilateral dorsal root rhizotomy at the L6 level, which showed significant increases in threshold pressure (rhizotomized vs. control: 14.25 ± 0.82 vs. 8.40 ± 0.69 cmH2O, P <0.01, n = 28), post-voiding pressure (7.66 ± 0.56 vs. 5.42 ± 0.52 cmH2O, P <0.01, n = 28), holding duration (135.06 ± 23.6 vs. 77.73 ± 13.56 sec, P <0.05, n = 28), and inter-contraction interval (140.62 ± 23.29 vs. 82.40 ± 13.57 sec, P <0.05, n = 28). Several (mean = 2.32 ± 1.31 vs. 0.12 ± 0.21, P <0.01, n = 28, P <0.01, n = 28) non-voiding contractions with gradual increase in IVP were found ahead of voiding contraction after rhizotomy. An additional dorsal root rhizotomy at the ipsilateral S1 level caused further increases in urodynamic parameters (threshold pressure, 18.18 ± 1.67 cmH2O, P <0.01; post-voiding pressure 8.07 ± 0.96 cmH2O, P <0.01; holding duration, 211.44 ± 42.54 sec, P <0.01; inter-contraction interval, 264.2 ± 59.99 sec, P <0.05; non-voiding contractions, 4.41 ± 2.12, P <0.01, n = 7). Intrathecal glutamate (100 μM, 10 μl) ameliorated all the pathological conditions induced by unilateral dorsal root rhizotomy at the L6 level in a dose dependent manner (ED50 = 1.25 ± 10-5). Intrathecal CNQX (6-cyano-7-nitroquinoxaline-2,3- dione; 100 μM, 10 μl) and APV (D-2-amino-5-phosphonovaleric acid; 100 μM, 10 μl) injections after rhizotomy at the L6 level induced disturbances similar to that caused by an additional rhizotomy at ipsilateral S1 level. Wherease, glutamate (100 μM, 10 μl) reversed the disturbances caused by CNQX but showed no effect on that by APV. Conclusions: Acute partial sensory deprivation caused acute impaired micturition reflex in rat models. In addition, glutamatergic NMDA and AMPA receptors are important for mediating these impairments in micturition reflex.

KW - AMPA

KW - Glutamate

KW - NMDA

KW - Sensory

KW - Voiding

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