Effects of rosiglitazone on native low-density-lipoprotein-induced respiratory burst in circulating monocytes and on the leukocyte-endothelial interaction in cholesterol-fed rats

Long Sheng Lu, Li Man Hung, Chang Hui Liao, Chau Chung Wu, Ming Jai Su

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Low-density lipoprotein (LDL) has been implicated in the initiation and progression of atherosclerotic vascular disease. But whether LDL can elicit similar effects in the microcirculation remain unexplored. To approach this issue, the hypothesis that LDL promotes oxidative stress in monocytes and results in microvascular inflammation was tested. Native LDL was capable of stimulating respiratory burst in rat monocytes, and this was blocked by BAPTA, cytochalasin B, apocynin, and diphenyliodonium. In monocytes from rats on a high-cholesterol (4%) diet, increased intracellular calcium, actin polymerization, respiratory burst, and surface CD18 expression were found. Concurrently, leukocyte-endothelial interaction was enhanced in the cremaster microcirculation. Rosiglitazone, an insulin-sensitizing agent with antiinflammatory properties, was found to suppress native-LDL-induced actin polymerization and respiratory burst in monocytes. It also improved leukocytes activation and leukocyte-endothelial interaction due to the high cholesterol intake. Hence, native LDL stimulation of monocytes contributed to hypercholesterolemia-associated microvascular inflammation, which could be treated by rosiglitazone.

Original languageEnglish
Pages (from-to)251-260
Number of pages10
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume375
Issue number4
DOIs
Publication statusPublished - Jun 2007
Externally publishedYes

Keywords

  • Free radicals
  • Hypercholesterolemia
  • Leukocyte-endothelial interaction
  • Monocytes
  • Native low density lipoprotein
  • Rosiglitazone

ASJC Scopus subject areas

  • Pharmacology

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