Abstract

Aim: To quantify the effects of pulsed radiofrequency (PRF) electrical stimulations of the pudendal and pelvic nerves on the bladder function of rats with detrusor overactivity. Methods: All rats were pretreated with a continuous transvesical infusion of 0.5% acetic acid (AA) for inducing detrusor overactivity. Intravesical pressure was recorded using cysometrography (CMG) during the continuous transvesical infusion to examine the effects of PRF electrical stimulation of the pudendal and pelvic nerves individually. In addition, the activity of caspase-3, an apoptosis marker, in the pelvic nerve was examined to evaluate the impact of PRF on nerve injury. Results: According to the first CMG recording, AA treatment significantly reduced bladder capacity (BC) and intercontraction interval (ICI) to 65% and 66% of the corresponding control values, respectively. Subsequently, PRF electrical stimulation of the pelvic nerve inhibited AA-induced detrusor overactivity and significantly increased BC to approximately 102-110% and ICI to 79-92%; these effects persisted for at least 4 h. Furthermore, PRF did not cause significant neural damage to the target stimulated nerves, as demonstrated by caspase-3 activity. Conclusion: PRF electrical stimulation of pelvic nerves exerted a long-lasting effect of suppressing AA-induced detrusor overactivity. This modality can be used as an alternative approach for improving bladder continence in patients with overactive bladder syndrome. © 2017 Wiley Periodicals, Inc.
Original languageEnglish
Pages (from-to)1734-1741
JournalNeurourology and Urodynamics
Volume36
Issue number7
DOIs
Publication statusPublished - Sep 7 2017

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Overactive Urinary Bladder
Acetic Acid
Electric Stimulation
Urinary Bladder
Pudendal Nerve
Caspase 3
Apoptosis
Pressure
Wounds and Injuries
Therapeutics

Keywords

  • Acetic acid
  • Electrical stimulation
  • Overactive bladder
  • Pelvic nerve
  • Pudendal nerve
  • Pulsed radiofrequency

Cite this

Jen, E., Hsieh, T., Lu, T. C., Chen, M. C., Lee, F. J., Lin, C. T., ... Lin, C. W. (2017). Effects of pulsed-radiofrequency neuromodulation on the rat with overactive bladder. Neurourology and Urodynamics, 36(7), 1734-1741. https://doi.org/10.1002/nau.23183

Effects of pulsed-radiofrequency neuromodulation on the rat with overactive bladder. / Jen, En; Hsieh, Tsung-hsun; Lu, Tsung Che; Chen, Meng Chao; Lee, Fu Jung; Lin, Chih Ting; Chen, Shih-Ching; Chu, Pei Yi; Peng, Chih-Wei; Lin, Chii Wann.

In: Neurourology and Urodynamics, Vol. 36, No. 7, 07.09.2017, p. 1734-1741.

Research output: Contribution to journalArticle

Jen, E, Hsieh, T, Lu, TC, Chen, MC, Lee, FJ, Lin, CT, Chen, S-C, Chu, PY, Peng, C-W & Lin, CW 2017, 'Effects of pulsed-radiofrequency neuromodulation on the rat with overactive bladder', Neurourology and Urodynamics, vol. 36, no. 7, pp. 1734-1741. https://doi.org/10.1002/nau.23183
Jen, En ; Hsieh, Tsung-hsun ; Lu, Tsung Che ; Chen, Meng Chao ; Lee, Fu Jung ; Lin, Chih Ting ; Chen, Shih-Ching ; Chu, Pei Yi ; Peng, Chih-Wei ; Lin, Chii Wann. / Effects of pulsed-radiofrequency neuromodulation on the rat with overactive bladder. In: Neurourology and Urodynamics. 2017 ; Vol. 36, No. 7. pp. 1734-1741.
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AB - Aim: To quantify the effects of pulsed radiofrequency (PRF) electrical stimulations of the pudendal and pelvic nerves on the bladder function of rats with detrusor overactivity. Methods: All rats were pretreated with a continuous transvesical infusion of 0.5% acetic acid (AA) for inducing detrusor overactivity. Intravesical pressure was recorded using cysometrography (CMG) during the continuous transvesical infusion to examine the effects of PRF electrical stimulation of the pudendal and pelvic nerves individually. In addition, the activity of caspase-3, an apoptosis marker, in the pelvic nerve was examined to evaluate the impact of PRF on nerve injury. Results: According to the first CMG recording, AA treatment significantly reduced bladder capacity (BC) and intercontraction interval (ICI) to 65% and 66% of the corresponding control values, respectively. Subsequently, PRF electrical stimulation of the pelvic nerve inhibited AA-induced detrusor overactivity and significantly increased BC to approximately 102-110% and ICI to 79-92%; these effects persisted for at least 4 h. Furthermore, PRF did not cause significant neural damage to the target stimulated nerves, as demonstrated by caspase-3 activity. Conclusion: PRF electrical stimulation of pelvic nerves exerted a long-lasting effect of suppressing AA-induced detrusor overactivity. This modality can be used as an alternative approach for improving bladder continence in patients with overactive bladder syndrome. © 2017 Wiley Periodicals, Inc.

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