Effects of Porphyromonas gingivalis on Titanium Surface by Different Clinical Treatment

Nominzul Batsukh, Sheng Wei Feng, Wei Fang Lee, Sy Jye Leu, Pei Yo Tsai, Kuo Ning Ho, Che Tong Lin, Ching Hua Su, Wei Jen Chang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

As peri-implantitis is associated with biofilm development, the characteristics of titanium implants may influence biofilm formation, and thereby increase the risk for inflammation. The objective of this study was to evaluate the effect of titanium surface roughness induced by various debridement methods of peri-implants, such as the use of an ultrasonic scaler, rubber polishing cup, gallium–aluminum–arsenide laser, and chlorhexidine (CHX) rinse, on Porphyromonas (P.) gingivalis. Surface debridement was performed by immersing titanium discs in CHX rinse for 24 h or treatment with a laser, polishing cup, or ultrasonic scaler for 60 s. Surface topography was examined using a profilometer. For the bacterial assay, specimens were inoculated with P. gingivalis for 2 h and incubated for 6, 12, and 24 h. After incubation, bacterial adhesion on the discs was quantified via spectrophotometric evaluation. Moreover, scanning electron microscopy (SEM) images were analyzed to quantify P. gingivalis colonization on the titanium surfaces. Data were analyzed using one-way analysis of variance and Pearson’s correlation test (p < 0.05). Scaled surfaces showed the highest surface roughness (Ra, p < 0.001). There was a significant positive correlation between Ra values and optical density measurements at all incubation times (p < 0.05). The quantitative evaluation of P. gingivalis attachment through SEM revealed that the amounts of bacteria were significantly lower in the control, laser, and CHX groups compared with those in the other groups (p < 0.05). Moreover, a significant positive correlation was found between Ra and attached P. gingivalis number obtained from SEM images (p < 0.05). In conclusion, polishing, CHX, and laser treatments of titanium surfaces provide the highest reduction in P. gingivalis biofilm mass and regrowth in vitro. This effect was enhanced as the smoothness of the titanium surface was increased.

Original languageEnglish
Pages (from-to)35-44
Number of pages10
JournalJournal of Medical and Biological Engineering
Volume37
Issue number1
DOIs
Publication statusPublished - Feb 1 2017

Fingerprint

Porphyromonas gingivalis
Titanium
Chlorhexidine
Biofilms
Lasers
Polishing
Electron Scanning Microscopy
Debridement
Ultrasonics
Scanning electron microscopy
Peri-Implantitis
Surface roughness
Bacterial Adhesion
Density (optical)
Rubber
Surface topography
Analysis of variance (ANOVA)
Assays
Bacteria
Analysis of Variance

Keywords

  • Chlorhexidine (CHX)
  • Gallium–aluminum–arsenide (GaAlAs) laser
  • Mechanical debridement
  • Porphyromonas gingivalis (P. gingivalis)
  • Surface roughness
  • Titanium

ASJC Scopus subject areas

  • Medicine(all)
  • Biomedical Engineering

Cite this

Effects of Porphyromonas gingivalis on Titanium Surface by Different Clinical Treatment. / Batsukh, Nominzul; Feng, Sheng Wei; Lee, Wei Fang; Leu, Sy Jye; Tsai, Pei Yo; Ho, Kuo Ning; Lin, Che Tong; Su, Ching Hua; Chang, Wei Jen.

In: Journal of Medical and Biological Engineering, Vol. 37, No. 1, 01.02.2017, p. 35-44.

Research output: Contribution to journalArticle

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abstract = "As peri-implantitis is associated with biofilm development, the characteristics of titanium implants may influence biofilm formation, and thereby increase the risk for inflammation. The objective of this study was to evaluate the effect of titanium surface roughness induced by various debridement methods of peri-implants, such as the use of an ultrasonic scaler, rubber polishing cup, gallium–aluminum–arsenide laser, and chlorhexidine (CHX) rinse, on Porphyromonas (P.) gingivalis. Surface debridement was performed by immersing titanium discs in CHX rinse for 24 h or treatment with a laser, polishing cup, or ultrasonic scaler for 60 s. Surface topography was examined using a profilometer. For the bacterial assay, specimens were inoculated with P. gingivalis for 2 h and incubated for 6, 12, and 24 h. After incubation, bacterial adhesion on the discs was quantified via spectrophotometric evaluation. Moreover, scanning electron microscopy (SEM) images were analyzed to quantify P. gingivalis colonization on the titanium surfaces. Data were analyzed using one-way analysis of variance and Pearson’s correlation test (p < 0.05). Scaled surfaces showed the highest surface roughness (Ra, p < 0.001). There was a significant positive correlation between Ra values and optical density measurements at all incubation times (p < 0.05). The quantitative evaluation of P. gingivalis attachment through SEM revealed that the amounts of bacteria were significantly lower in the control, laser, and CHX groups compared with those in the other groups (p < 0.05). Moreover, a significant positive correlation was found between Ra and attached P. gingivalis number obtained from SEM images (p < 0.05). In conclusion, polishing, CHX, and laser treatments of titanium surfaces provide the highest reduction in P. gingivalis biofilm mass and regrowth in vitro. This effect was enhanced as the smoothness of the titanium surface was increased.",
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