Effects of Polypropylene Carbonate/Poly(d,l-lactic) Acid/Tricalcium Phosphate Elastic Composites on Improving Osteoblast Maturation

Hsu Wei Fang, Wei Yu Kao, Pei I. Lin, Guang Wei Chang, Ya Jung Hung, Ruei Ming Chen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bone tissue engineering utilizing biomaterials to improve osteoblast growth has provided de novo consideration for therapy of bone diseases. Polypropylene carbonate (PPC) is a polymer with a low glass transition temperature but high elasticity. In this study, we developed a new PPC-derived composite by mixing poly-lactic acid (PLA) and tricalcium phosphate (TCP), called PPC/PLA/TCP elastic (PPTE) scaffolds. We also evaluated the beneficial effects of PPTE composites on osteoblast growth and maturation and the possible mechanisms. Compared to PPC polymers, PPTE composites had similar pore sizes and porosities but possessed better hydrophilic surface structures. Biological evaluations further revealed that PPTE composites attracted adhesion of mouse osteoblasts, and these bone cells extended along the porous scaffolds to produce accurate fibroblast-like morphologies. In parallel, seeding mouse osteoblasts onto PPTE composites time-dependently increased cell growth. Sequentially, PPTE composites augmented DNA replication and cell proliferation. Consequently, PPTE composites significantly improved osteoblast mineralization. As to the mechanism, treatment with PPTE composites induced osteopontin (OPN) mRNA and protein expression and alkaline phosphatase activity. Taken together, this study showed that PPTE composites with porous and hydrophilic surfaces can stimulate osteoblast adhesion, proliferation, and maturation through an OPN-dependent mechanism. Therefore, the de novo PPTE scaffolds may have biomaterial potential for bone regeneration.

Original languageEnglish
Pages (from-to)1999-2009
Number of pages11
JournalAnnals of Biomedical Engineering
Volume43
Issue number8
DOIs
Publication statusPublished - Aug 25 2015

Keywords

  • Bone regeneration
  • Cell proliferation and maturation
  • Osteoblasts
  • Osteopontin
  • PPC/PLA/TCP elastic composites

ASJC Scopus subject areas

  • Biomedical Engineering

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