Effects of parenteral infusion with fish-oil or safflower-oil emulsion on hepatic lipids, plasma amino acids, and inflammatory mediators in septic rats

Che Yi Chao, Sung Ling Yeh, Ming Tsan Lin, Wei Jao Chen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

This study was designed to investigate the effects of preinfusion with total parenteral nutrition (TPN) using fish-oil (FO) versus safflower-oil (SO) emulsion as fat sources on hepatic lipids, plasma amino-acid profiles, and inflammatory-related mediators in septic rats. Normal rats, with internal jugular catheters, were assigned to two different groups and received TPN. TPN provided 300 kcal . kg-1 . d-1, with 40% of the non-protein energy as fat. All TPN solutions were isonitrogenous and identical in nutrient composition except for the fat emulsion, which was made of SO or FO. After receiving TPN for 6 d, each group of rats was further divided into control and sepsis subgroups. Sepsis was induced by cecal ligation and puncture; control rats received sham operation. All rats were classified into four groups as follows: FO control group (FOC; n = 7), FO sepsis group (FOS; n = 8), SO control group (SOC; n = 8), and SO sepsis group (SOS; n = 9). The results of the study demonstrated that plasma concentrations of triacylglycerol and non-esterified fatty acids did not differ between the FO and SO groups, regardless of whether the animals were septic. SOS had significantly higher total lipids and cholesterol content in the liver than did the SOC group. The FOS group, however, showed no difference from the FOC group. Plasma leucine and isoleucine levels were significantly lower in the SOS group than in the SOC group, whereas no difference in these two amino acids was observed between the FOC and FOS groups. Plasma arginine levels were significantly lower in both septic groups than in the groups without sepsis when either FO or SO was infused. Plasma glutamine levels, however, did not differ across groups. No differences in interleukin-1β, interleukin-6, tumor necrosis factor-α, or leukotriene B4 concentrations in peritoneal lavage fluid were observed between the two septic groups. These results suggest that catabolic reaction in septic rats preinfused with FO is not as obvious as those preinfused with SO. Compared with SO emulsion, TPN with FO emulsion prevents liver fat accumulation associated with sepsis. However, parenterally administered FO had no beneficial effect in lowering cytokines and LTB4 levels in peritoneal lavage fluid in septic rats induced by cecal ligation and puncture. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)284-288
Number of pages5
JournalNutrition
Volume16
Issue number4
DOIs
Publication statusPublished - Apr 2000

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Parenteral Infusions
Safflower Oil
Fish Oils
Emulsions
Total Parenteral Nutrition
Lipids
Amino Acids
Liver
Sepsis
Fats
Peritoneal Lavage
Leukotriene B4
Ascitic Fluid
Punctures
Ligation
Parenteral Nutrition Solutions
Control Groups
Isoleucine
Glutamine
Interleukin-1

Keywords

  • Cytokine
  • Fish oil
  • Lipids
  • Parenteral nutrition
  • Safflower oil
  • Sepsis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Surgery
  • Medicine (miscellaneous)

Cite this

Effects of parenteral infusion with fish-oil or safflower-oil emulsion on hepatic lipids, plasma amino acids, and inflammatory mediators in septic rats. / Chao, Che Yi; Yeh, Sung Ling; Lin, Ming Tsan; Chen, Wei Jao.

In: Nutrition, Vol. 16, No. 4, 04.2000, p. 284-288.

Research output: Contribution to journalArticle

Chao, Che Yi ; Yeh, Sung Ling ; Lin, Ming Tsan ; Chen, Wei Jao. / Effects of parenteral infusion with fish-oil or safflower-oil emulsion on hepatic lipids, plasma amino acids, and inflammatory mediators in septic rats. In: Nutrition. 2000 ; Vol. 16, No. 4. pp. 284-288.
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N2 - This study was designed to investigate the effects of preinfusion with total parenteral nutrition (TPN) using fish-oil (FO) versus safflower-oil (SO) emulsion as fat sources on hepatic lipids, plasma amino-acid profiles, and inflammatory-related mediators in septic rats. Normal rats, with internal jugular catheters, were assigned to two different groups and received TPN. TPN provided 300 kcal . kg-1 . d-1, with 40% of the non-protein energy as fat. All TPN solutions were isonitrogenous and identical in nutrient composition except for the fat emulsion, which was made of SO or FO. After receiving TPN for 6 d, each group of rats was further divided into control and sepsis subgroups. Sepsis was induced by cecal ligation and puncture; control rats received sham operation. All rats were classified into four groups as follows: FO control group (FOC; n = 7), FO sepsis group (FOS; n = 8), SO control group (SOC; n = 8), and SO sepsis group (SOS; n = 9). The results of the study demonstrated that plasma concentrations of triacylglycerol and non-esterified fatty acids did not differ between the FO and SO groups, regardless of whether the animals were septic. SOS had significantly higher total lipids and cholesterol content in the liver than did the SOC group. The FOS group, however, showed no difference from the FOC group. Plasma leucine and isoleucine levels were significantly lower in the SOS group than in the SOC group, whereas no difference in these two amino acids was observed between the FOC and FOS groups. Plasma arginine levels were significantly lower in both septic groups than in the groups without sepsis when either FO or SO was infused. Plasma glutamine levels, however, did not differ across groups. No differences in interleukin-1β, interleukin-6, tumor necrosis factor-α, or leukotriene B4 concentrations in peritoneal lavage fluid were observed between the two septic groups. These results suggest that catabolic reaction in septic rats preinfused with FO is not as obvious as those preinfused with SO. Compared with SO emulsion, TPN with FO emulsion prevents liver fat accumulation associated with sepsis. However, parenterally administered FO had no beneficial effect in lowering cytokines and LTB4 levels in peritoneal lavage fluid in septic rats induced by cecal ligation and puncture. Copyright (C) 2000 Elsevier Science Inc.

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