Effects of overexpression of IL-10, IL-12, TGF-β and IL-4 on allergen induced change in bronchial responsiveness

Chi Ling Fu, Yi Ling Ye, Yueh L. Lee, Bor Luen Chiang

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Background: An increasing prevalence of allergic diseases, such as atopic dermatitis, allergic rhinitis and bronchial asthma, has been noted worldwide. Allergic asthma strongly correlates with airway inflammation caused by the unregulated production of cytokines secreted by allergen-specific type-2 T helper (Th2) cells. This study aims to explore the therapeutic effect of the airway gene transfer of IL-12, IL-10 and TGF-β on airway inflammation in a mouse model of allergic asthma. Methods: BALB/c mice were sensitized to ovalbumin (OVA) by intr aperitoneal injections with OVA and challenged by nebulized OVA. Different cytokine gene plasmids or non-coding vector plasmids were instilled daily into the trachea up to one day before the inhalatory OVA challenge phase. Results: Intratracheal administration of IL-10, IL-12 or TGF-β can efficiently inhibit antigen-induced airway hyper-responsiveness and is able to largely significantly lower the number of eosinophils and neutrophils in bronchoalveolar lavage fluid of ovalbumin (OVA) sensitized and challenged mice during the effector phase. Furthermore, the effect of IL-10 plasmids is more remarkable than any other cytokine gene plasmid. On the other hand, local administration of IL-4 gene plasmids before antigen challenge can induce severe airway hyper-responsiveness (AHR) and airway eosinophilia. Conclusion: Our data demonstrated that anti-inflammatory cytokines, particularly IL-10, have the therapeutic potential for the alleviation of airway inflammation in murine model of asthma.

Original languageEnglish
Article number72
JournalRespiratory Research
Volume7
DOIs
Publication statusPublished - May 8 2006

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Ovalbumin
Interleukin-12
Interleukin-4
Interleukin-10
Allergens
Plasmids
Respiratory Hypersensitivity
Asthma
Cytokines
Inflammation
Genes
Antigens
Th2 Cells
Bronchoalveolar Lavage Fluid
Eosinophilia
Therapeutic Uses
Atopic Dermatitis
Helper-Inducer T-Lymphocytes
Trachea
Eosinophils

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Effects of overexpression of IL-10, IL-12, TGF-β and IL-4 on allergen induced change in bronchial responsiveness. / Fu, Chi Ling; Ye, Yi Ling; Lee, Yueh L.; Chiang, Bor Luen.

In: Respiratory Research, Vol. 7, 72, 08.05.2006.

Research output: Contribution to journalArticle

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