Effects of low molecular weight chitosans on aristolochic acid-induced renal lesions in mice

Ya Min Chang, Chen Tien Chang, Tzu Chuan Huang, Shih Ming Chen, Jen Ai Lee, Yun Chin Chung

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This study investigated the effects of low molecular weight chitosan (LMWC) on the renal functions for aristolochic acid (AA)-induced renal lesions in C3H/He mice. Commercial crab shell chitosan (molecular mass of ∼1100 kDa) was hydrolysed using bamboo chitosanase at pH 3.5 and 50 °C for 18 h. LMWC (with molecular mass of ∼29 kDa) was isolated from the hydrolysate through 50% methanol fractionation. The levels of urinary microalbumin, urinary-N-acetyl-beta-d-glucosaminidase, serum creatinine, and blood urea nitrogen were higher in the AA induced mice than those in the control group, while the rate for creatinine clearance was lower in the AA induced mice. Oral administration of LMWC could suppress the elevation of urinary and blood nitrogen concentrations induced by AA treatment. Renal histopathological examination revealed that AA-induced renal alterations were reverted effectively by the treatment of LMWC at three tested doses, especially at the moderate dose of 500 mg/kg BW.

Original languageEnglish
Pages (from-to)1751-1758
Number of pages8
JournalFood Chemistry
Volume129
Issue number4
DOIs
Publication statusPublished - Dec 15 2011

    Fingerprint

Keywords

  • Aristolochic acid
  • Bamboo chitosanase
  • Low molecular weight chitosans
  • Renal histopathology
  • Renal lesions

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this