Effects of lipoproteins and insulin on phospholipid transfer protein and gene transcription in Hep G2 cells

Y. H. Tsai, J. C J Chao, A. Y. Tu, M. J. Shieh

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to investigate the effects of lipoproteins and insulin on phospholipid transfer protein (PLTP) content and gene transcription in human hepatoblastoma (Hep G2) cells. Upon 90% confluency, the cells were switched to serum-free medium for 24 hours followed by incubation with HDL (50 μg/mL), LDL (50 μg/mL), VLDL (50 μg/mL) or insulin (1 μg/mL) for 12-24 hours. The conditioned medium was collected at 12 and 24 hours for protein analysis. Cells were collected after 24 hours for the measurements of PLTP content and mRNA expression. The results showed that the total protein secretion into the conditioned medium in the LDL, VLDL and insulin treatment groups was significantly lower than the control group at both 12 and 24 hours (p

Original languageEnglish
JournalFASEB Journal
Volume12
Issue number5
Publication statusPublished - Mar 20 1998

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Phospholipid Transfer Proteins
Hep G2 Cells
Transcription
lipoproteins
Lipoproteins
insulin
transcription (genetics)
Genes
Insulin
Conditioned Culture Medium
protein content
Hepatoblastoma
protein secretion
genes
Serum-Free Culture Media
cells
Proteins
Control Groups
Messenger RNA
lipid transfer proteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Effects of lipoproteins and insulin on phospholipid transfer protein and gene transcription in Hep G2 cells. / Tsai, Y. H.; Chao, J. C J; Tu, A. Y.; Shieh, M. J.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

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