Large amounts of Ganoderma lucidum (GL) commercial products are provided in the worldwide market such as powders, tea bags, or capsules as dietary supplements which contained triterpenoids and/or polysaccharides. Therefore, it was estimated that several thousand tons of GL residues (GLR) are produced and discarded. For recycling uses, the aim of this study was to evaluate the benefits of two hot-water extracts from GLR (HWPGLR) and solid-state fermentation GLR inoculated with GL mycelia (HWPGLRF) on the growths of Lactobacillus rhamnosus and Bifidobacterium longum. The RAW264.7 cells were used to investigate the effects of HWPGLR and HWPGLRF on nitric oxide productions, phagocytic activities against FITC-labeled E. coli, and to lower lipopolysaccharide (LPS)-binding capacities. The powders of GLR and GLRF were used as additives in the commercial feeds for feeding broiler chicks in vivo to evaluate the immune-stimulatory and prebiotic activities.