Effects of growth factors and granulosa cell co-culture on in-vitro maturation of oocytes

Yu Hung Lin, Jiann-Loung Hwang, Kok Min Seow, Lee Wen Huang, Heng Ju Chen, Chii Ruey Tzeng

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The maturation medium for in-vitro oocyte maturation is usually supplemented with serum. However, supplementation with serum from pregnant women adversely affects the outcome of in-vitro maturation. The purpose of the study was to assess if growth factors or granulosa cell co-culture could overcome the adverse effects of pregnant women's serum. The basal maturation medium consisted of TCM199, 75 mIU/ml human menopausal gonadotrophin (HMG), 0.2 mmol/l pyruvate, and 10% serum. The maturation medium for control 1 contained fertile women's serum. The maturation medium for control 2 contained pregnant women's serum. The maturation media for the study groups consisted of medium for control 2, with the addition of EGF, IGF-I, activin, TGFβ or granulosa cell co-culture. Immature oocytes were obtained from FVB mice, and the experiment was repeated six times. After maturation, the oocytes were fertilized and cultured to blastocysts, and the cumulus cells were analysed for apoptosis. The maturation, fertilization and blastocyst rates of the control 2 group were significantly lower than those of control 1 group (P <0.05). Addition of EGF, IGF-1, activin, TGFβ or granulosa cell co-culture could not improve the outcome of in-vitro maturation. Cumulus cell proliferation was inhibited by pregnant women's serum. Apoptosis of cumulus cell was not related to in-vitro oocyte maturation and subsequent embryo development.

Original languageEnglish
Pages (from-to)165-170
Number of pages6
JournalReproductive BioMedicine Online
Volume19
Issue number2
DOIs
Publication statusPublished - Aug 2009

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In Vitro Oocyte Maturation Techniques
Granulosa Cells
Coculture Techniques
Intercellular Signaling Peptides and Proteins
Cell Culture Techniques
Cumulus Cells
Serum
Pregnant Women
Activins
Blastocyst
Insulin-Like Growth Factor I
Epidermal Growth Factor
Oocytes
Apoptosis
Menotropins
Control Groups
Pyruvic Acid
Fertilization
Embryonic Development
Cell Proliferation

Keywords

  • Apoptosis
  • Co-culture
  • Granulosa cell
  • Growth factor
  • In-vitro maturation

ASJC Scopus subject areas

  • Reproductive Medicine
  • Developmental Biology

Cite this

Effects of growth factors and granulosa cell co-culture on in-vitro maturation of oocytes. / Lin, Yu Hung; Hwang, Jiann-Loung; Seow, Kok Min; Huang, Lee Wen; Chen, Heng Ju; Tzeng, Chii Ruey.

In: Reproductive BioMedicine Online, Vol. 19, No. 2, 08.2009, p. 165-170.

Research output: Contribution to journalArticle

Lin, Yu Hung ; Hwang, Jiann-Loung ; Seow, Kok Min ; Huang, Lee Wen ; Chen, Heng Ju ; Tzeng, Chii Ruey. / Effects of growth factors and granulosa cell co-culture on in-vitro maturation of oocytes. In: Reproductive BioMedicine Online. 2009 ; Vol. 19, No. 2. pp. 165-170.
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