Effects of glycolic acid on the induction of apoptosis via caspase-3 activation in human leukemia cell line (HL-60)

Jen Hung Yang, Chi Chung Chou, Ya Wen Cheng, Lee Yan Sheen, Ming Chi Chou, Hsin Su Yu, Yau Huei Wei, Jing Gung Chung

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Apoptosis is a particular process that leads to the programmed cell death, and it has been a potentially therapeutic target of cancer. In this study, we evaluated the possible apoptotic effects of glycolic acid on human leukemia cell line (HL-60) in vitro. The morphological changes, cell viability, apoptosis induction, and caspase-3 activity were measured by phase microscopy, flow cytometry, and Western blot analysis. Morphological changes including shrinkage of cells were clearly demonstrated in HL-60 cells treated with increasing concentrations of glycolic acid. Cell viability was significantly affected by glycolic acid treatment in a dose- and time-dependent manner. In comparison to the control group, glycolic acid treatment had a profound effect in the induction of apoptosis by flow cytometric assays. In the cell cycle analysis, glycolic acid caused the increased percentage of cells in G2/M phase and the decreased expression of the cyclin A and cyclin B1, suggesting the induction of G2/M arrest of cell cycle by glycolic acid. Moreover, glycolic acid treatment promoted caspase-9 and -3 activity in a dose-dependent manner, but caspse-8 activity was not affected during the same process. Glycolic acid co-administrated with broad-spectrum caspase inhibitor, z-VAD-fmk, caspase-3 activity was blunted and apoptosis was also markedly blocked in HL-60 cells. In conclusion, glycolic acid-induced apoptosis in HL-60 cells may be through the activation of caspase-3. Future studies focusing on cell signaling and biological significance of glycolic acid-induced apoptosis would lead to exploring the mechanisms of chemotherapeutic potency of glycolic acid in human cancers.

Original languageEnglish
Pages (from-to)1777-1784
Number of pages8
JournalFood and Chemical Toxicology
Volume42
Issue number11
DOIs
Publication statusPublished - Nov 2004
Externally publishedYes

Fingerprint

glycolic acid
caspase-3
leukemia
Caspase 3
Leukemia
apoptosis
Chemical activation
Cells
cell lines
Apoptosis
Cell Line
HL-60 Cells
acid treatment
cyclins
cells
cell viability
Cell Survival
G2 Phase Cell Cycle Checkpoints
Cell signaling
Cyclin B1

Keywords

  • Apoptosis
  • Flow cytometry
  • Glycolic acid
  • Human leukemia HL-60 cells

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Yang, J. H., Chou, C. C., Cheng, Y. W., Sheen, L. Y., Chou, M. C., Yu, H. S., ... Chung, J. G. (2004). Effects of glycolic acid on the induction of apoptosis via caspase-3 activation in human leukemia cell line (HL-60). Food and Chemical Toxicology, 42(11), 1777-1784. https://doi.org/10.1016/j.fct.2004.07.004

Effects of glycolic acid on the induction of apoptosis via caspase-3 activation in human leukemia cell line (HL-60). / Yang, Jen Hung; Chou, Chi Chung; Cheng, Ya Wen; Sheen, Lee Yan; Chou, Ming Chi; Yu, Hsin Su; Wei, Yau Huei; Chung, Jing Gung.

In: Food and Chemical Toxicology, Vol. 42, No. 11, 11.2004, p. 1777-1784.

Research output: Contribution to journalArticle

Yang, JH, Chou, CC, Cheng, YW, Sheen, LY, Chou, MC, Yu, HS, Wei, YH & Chung, JG 2004, 'Effects of glycolic acid on the induction of apoptosis via caspase-3 activation in human leukemia cell line (HL-60)', Food and Chemical Toxicology, vol. 42, no. 11, pp. 1777-1784. https://doi.org/10.1016/j.fct.2004.07.004
Yang, Jen Hung ; Chou, Chi Chung ; Cheng, Ya Wen ; Sheen, Lee Yan ; Chou, Ming Chi ; Yu, Hsin Su ; Wei, Yau Huei ; Chung, Jing Gung. / Effects of glycolic acid on the induction of apoptosis via caspase-3 activation in human leukemia cell line (HL-60). In: Food and Chemical Toxicology. 2004 ; Vol. 42, No. 11. pp. 1777-1784.
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