Effects of glutamine on adhesion molecule expression and leukocyte transmigration in endothelial cells exposed to arsenic

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Abstract

This study evaluated whether glutamine (GLN) concentration was related to endothelial surface molecule expression and the migration of polymorphonuclear neutrophils (PMNs) through endothelial cells (ECs) stimulated by arsenic. Human umbilical vein endothelial cells (HUVECs) and PMNs were treated with different GLN concentrations (0, 300, 600 and 1000 μM) for 24 h. After that, we stimulated HUVECs for 3 h with 0.5 μM arsenic, and PMNs were allowed to transmigrate to ECs for 2 h. HUVEC surface expressions of cell adhesion molecules and integrin (CD11b) and interleukin (IL)-8 receptor expressions on PMNs were measured. The transendothelial migration of PMNs was also analyzed. The results showed that cell adhesion molecule (CAM) and integrin expressions in arsenic groups were higher than in those without arsenic. Among the arsenic groups, the expression of CAMs on ECs and CD11b, and IL-8 receptor on PMNs was lowest with 0 μM compared with the other GLN concentrations. Vascular CAM-1 on ECs and CD11b on PMN expression were higher with 300 μM than with 600 and 1000 μM GLN. IL-8 secretions from ECs and PMNs were higher with 300 μM than with 600 and 1000 μM GLN, and this was consistent with the expression of the IL-8 receptor on PMNs. Polymorphonuclear neutrophil transmigration was significantly higher with 300 μM GLN than with other GLN concentrations. These results suggest that ECs and PMNs were activated after arsenic stimulation. Cell adhesion molecule expressions on ECs and PMNs were suppressed in the absence of GLN. A low GLN concentration comparable to catabolic conditions resulted in higher adhesion molecule expression and greater transendothelial migration of neutrophils. Glutamine administration at levels similar to or higher than physiological concentrations reduced IL-8 and adhesion molecule expression; PMN transmigration was also decreased after stimulation with arsenic.

Original languageEnglish
Pages (from-to)700-704
Number of pages5
JournalJournal of Nutritional Biochemistry
Volume16
Issue number11
DOIs
Publication statusPublished - Nov 2005

Fingerprint

Endothelial cells
Cell Adhesion Molecules
Arsenic
Glutamine
Neutrophils
Adhesion
Endothelial Cells
Molecules
Interleukin-8 Receptors
Human Umbilical Vein Endothelial Cells
Interleukin-8
Integrins
Transendothelial and Transepithelial Migration
Vascular Cell Adhesion Molecule-1
Computer aided manufacturing

Keywords

  • Adhesion molecule expression
  • Arsenic exposure
  • Glutamine
  • Interleukin-8
  • Polymorphonuclear neutrophils transmigration

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

@article{0e9f433828f444f4ac83a9bc9ec7c3ba,
title = "Effects of glutamine on adhesion molecule expression and leukocyte transmigration in endothelial cells exposed to arsenic",
abstract = "This study evaluated whether glutamine (GLN) concentration was related to endothelial surface molecule expression and the migration of polymorphonuclear neutrophils (PMNs) through endothelial cells (ECs) stimulated by arsenic. Human umbilical vein endothelial cells (HUVECs) and PMNs were treated with different GLN concentrations (0, 300, 600 and 1000 μM) for 24 h. After that, we stimulated HUVECs for 3 h with 0.5 μM arsenic, and PMNs were allowed to transmigrate to ECs for 2 h. HUVEC surface expressions of cell adhesion molecules and integrin (CD11b) and interleukin (IL)-8 receptor expressions on PMNs were measured. The transendothelial migration of PMNs was also analyzed. The results showed that cell adhesion molecule (CAM) and integrin expressions in arsenic groups were higher than in those without arsenic. Among the arsenic groups, the expression of CAMs on ECs and CD11b, and IL-8 receptor on PMNs was lowest with 0 μM compared with the other GLN concentrations. Vascular CAM-1 on ECs and CD11b on PMN expression were higher with 300 μM than with 600 and 1000 μM GLN. IL-8 secretions from ECs and PMNs were higher with 300 μM than with 600 and 1000 μM GLN, and this was consistent with the expression of the IL-8 receptor on PMNs. Polymorphonuclear neutrophil transmigration was significantly higher with 300 μM GLN than with other GLN concentrations. These results suggest that ECs and PMNs were activated after arsenic stimulation. Cell adhesion molecule expressions on ECs and PMNs were suppressed in the absence of GLN. A low GLN concentration comparable to catabolic conditions resulted in higher adhesion molecule expression and greater transendothelial migration of neutrophils. Glutamine administration at levels similar to or higher than physiological concentrations reduced IL-8 and adhesion molecule expression; PMN transmigration was also decreased after stimulation with arsenic.",
keywords = "Adhesion molecule expression, Arsenic exposure, Glutamine, Interleukin-8, Polymorphonuclear neutrophils transmigration",
author = "Yu-Chen Hou and Chun-Sen Hsu and Yeh, {Chiu Li} and Chiu, {Wan Chun} and Pai, {Man Hui} and Yeh, {Sung Ling}",
year = "2005",
month = "11",
doi = "10.1016/j.jnutbio.2005.04.007",
language = "English",
volume = "16",
pages = "700--704",
journal = "Journal of Nutritional Biochemistry",
issn = "0955-2863",
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T1 - Effects of glutamine on adhesion molecule expression and leukocyte transmigration in endothelial cells exposed to arsenic

AU - Hou, Yu-Chen

AU - Hsu, Chun-Sen

AU - Yeh, Chiu Li

AU - Chiu, Wan Chun

AU - Pai, Man Hui

AU - Yeh, Sung Ling

PY - 2005/11

Y1 - 2005/11

N2 - This study evaluated whether glutamine (GLN) concentration was related to endothelial surface molecule expression and the migration of polymorphonuclear neutrophils (PMNs) through endothelial cells (ECs) stimulated by arsenic. Human umbilical vein endothelial cells (HUVECs) and PMNs were treated with different GLN concentrations (0, 300, 600 and 1000 μM) for 24 h. After that, we stimulated HUVECs for 3 h with 0.5 μM arsenic, and PMNs were allowed to transmigrate to ECs for 2 h. HUVEC surface expressions of cell adhesion molecules and integrin (CD11b) and interleukin (IL)-8 receptor expressions on PMNs were measured. The transendothelial migration of PMNs was also analyzed. The results showed that cell adhesion molecule (CAM) and integrin expressions in arsenic groups were higher than in those without arsenic. Among the arsenic groups, the expression of CAMs on ECs and CD11b, and IL-8 receptor on PMNs was lowest with 0 μM compared with the other GLN concentrations. Vascular CAM-1 on ECs and CD11b on PMN expression were higher with 300 μM than with 600 and 1000 μM GLN. IL-8 secretions from ECs and PMNs were higher with 300 μM than with 600 and 1000 μM GLN, and this was consistent with the expression of the IL-8 receptor on PMNs. Polymorphonuclear neutrophil transmigration was significantly higher with 300 μM GLN than with other GLN concentrations. These results suggest that ECs and PMNs were activated after arsenic stimulation. Cell adhesion molecule expressions on ECs and PMNs were suppressed in the absence of GLN. A low GLN concentration comparable to catabolic conditions resulted in higher adhesion molecule expression and greater transendothelial migration of neutrophils. Glutamine administration at levels similar to or higher than physiological concentrations reduced IL-8 and adhesion molecule expression; PMN transmigration was also decreased after stimulation with arsenic.

AB - This study evaluated whether glutamine (GLN) concentration was related to endothelial surface molecule expression and the migration of polymorphonuclear neutrophils (PMNs) through endothelial cells (ECs) stimulated by arsenic. Human umbilical vein endothelial cells (HUVECs) and PMNs were treated with different GLN concentrations (0, 300, 600 and 1000 μM) for 24 h. After that, we stimulated HUVECs for 3 h with 0.5 μM arsenic, and PMNs were allowed to transmigrate to ECs for 2 h. HUVEC surface expressions of cell adhesion molecules and integrin (CD11b) and interleukin (IL)-8 receptor expressions on PMNs were measured. The transendothelial migration of PMNs was also analyzed. The results showed that cell adhesion molecule (CAM) and integrin expressions in arsenic groups were higher than in those without arsenic. Among the arsenic groups, the expression of CAMs on ECs and CD11b, and IL-8 receptor on PMNs was lowest with 0 μM compared with the other GLN concentrations. Vascular CAM-1 on ECs and CD11b on PMN expression were higher with 300 μM than with 600 and 1000 μM GLN. IL-8 secretions from ECs and PMNs were higher with 300 μM than with 600 and 1000 μM GLN, and this was consistent with the expression of the IL-8 receptor on PMNs. Polymorphonuclear neutrophil transmigration was significantly higher with 300 μM GLN than with other GLN concentrations. These results suggest that ECs and PMNs were activated after arsenic stimulation. Cell adhesion molecule expressions on ECs and PMNs were suppressed in the absence of GLN. A low GLN concentration comparable to catabolic conditions resulted in higher adhesion molecule expression and greater transendothelial migration of neutrophils. Glutamine administration at levels similar to or higher than physiological concentrations reduced IL-8 and adhesion molecule expression; PMN transmigration was also decreased after stimulation with arsenic.

KW - Adhesion molecule expression

KW - Arsenic exposure

KW - Glutamine

KW - Interleukin-8

KW - Polymorphonuclear neutrophils transmigration

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