Effects of fungal-derived high molecular weight chitosan on 5-fluorouracil-induced adverse reactions

Yih Huei Uen, Ying Jan Wang, Town Ming Hsu, Mei Huei Chen, Hing Yuen Chan, Yuan Soon Ho, Shyr Yi Lin

Research output: Contribution to journalArticle

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Abstract

The effects of fungal-derived high molecular weight chitosan (HMWC) on the prevention of 5-fluorouracil (5-FU)-induced side-effects were studied in a sarcoma-bearing mice model. 5-FU (12.5mg/kg) was gargled into the mice with or without HMWC (25, 75, and 150mg/kg) every 12 h for consecutive 8 or 16 days in different experiments. The HMWC (25-150 mg/kg) exerted no toxicity and did not interfere with the anti-tumor activity of 5-FU on sarcoma-bearing mice. HMWC in a higher dose (150 mg/kg) partially protected 5-FU-induced cytotoxicity on peripheral leukocytes, lymphocytes, and bone marrow CD-19 positive cells. HMWC reversed the 5-FU suppression of intestine sucrase activity and attenuated the 5-FU-induced diarrhea. Bone marrow cells micronucleus and DNA comet assays demonstrated that HMWC significantly reversed 5-FU-induced genome toxicity to marrow cells. These results suggested that fungal-derived HMWC attenuated the 5-FU-induced bone marrow and gastrointestinal toxicity, and has the potential for clinical applications in the future.

Original languageEnglish
Pages (from-to)458-472
Number of pages15
JournalJournal of Bioactive and Compatible Polymers
Volume23
Issue number5
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Chitosan
Fluorouracil
Molecular Weight
Molecular weight
Bearings (structural)
Toxicity
Bone
Cells
Bone Marrow
Sarcoma
Lymphocytes
Sucrase
Comet Assay
Cytotoxicity
Tumors
Assays
Bone Marrow Cells
DNA
Intestines
Genes

Keywords

  • 5-fluorouracil
  • Cancer chemotherapy
  • Chitosan
  • Comet assay
  • Cytotoxicity

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Effects of fungal-derived high molecular weight chitosan on 5-fluorouracil-induced adverse reactions. / Uen, Yih Huei; Wang, Ying Jan; Hsu, Town Ming; Chen, Mei Huei; Chan, Hing Yuen; Ho, Yuan Soon; Lin, Shyr Yi.

In: Journal of Bioactive and Compatible Polymers, Vol. 23, No. 5, 09.2008, p. 458-472.

Research output: Contribution to journalArticle

Uen, Yih Huei ; Wang, Ying Jan ; Hsu, Town Ming ; Chen, Mei Huei ; Chan, Hing Yuen ; Ho, Yuan Soon ; Lin, Shyr Yi. / Effects of fungal-derived high molecular weight chitosan on 5-fluorouracil-induced adverse reactions. In: Journal of Bioactive and Compatible Polymers. 2008 ; Vol. 23, No. 5. pp. 458-472.
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