Effects of flavonoids on the expression of the pro-inflammatory response in human monocytes induced by ligation of the receptor for AGEs

Shang Ming Huang, Chi Hao Wu, Gow Chin Yen

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Increasing evidence has shown advanced glycation end products (AGEs) receptor ligation (RAGE) to be an important part of complex interactions of the oxidative stress and pro-inflammatory responses. In this study, flavonoids were used to monitor the protective effects against the oxidative damage and inflammation mediated by AGEs in human monocytes. S100B (RAGE ligand) treatment in human THP-1 monocytic cells (THP-1) significantly increased gene expression of the pro-inflammatory cytokines TNF-α and IL-1β; chemokines MCP-1 and IP-10; adhesion factors platelet endothelial cell adhesion molecule (PECAM-1) and β2-integrin; and pro-inflammatory cyclooxygenase-2 (COX-2). S100B treatment with quercetin and catechin in THP-1 cells had inhibitory effects on the expression of pro-inflammatory genes and protein levels. Quercetin and catechin could regulate S100B-activated oxidant stress-sensitive pathways through blocking p47phox protein expression. Treatment with quercetin and catechin could eliminate reactive oxygen species (ROS) to reduce oxidative stress stimulated by S100B in THP-1 cells. Quercetin and catechin also showed different regulatory abilities on mitogen-activated protein kinase (MAPK) signaling pathways by inhibiting protein expression in S100B-stimulated inflammatory responses in THP-1 cells. This study suggests that quercetin and catechin may be of benefit for diabetic vascular complications due to its antioxidant abilities against AGE-mediated oxidative stress through oxidative stress-sensitive and oxidative stress-responsive signaling pathways, which lead to inflammation in human monocytes.

Original languageEnglish
Pages (from-to)1129-1139
Number of pages11
JournalMolecular Nutrition and Food Research
Volume50
Issue number12
DOIs
Publication statusPublished - Dec 2006
Externally publishedYes

Fingerprint

Catechin
Quercetin
catechin
Flavonoids
monocytes
quercetin
Ligation
Monocytes
Oxidative Stress
oxidative stress
flavonoids
inflammation
receptors
CD31 Antigens
protein synthesis
cells
Inflammation
Diabetic Angiopathies
Advanced Glycosylation End Products
Proteins

Keywords

  • AGEs
  • Catechin
  • Cytokine
  • Oxidative stress
  • Quercetin

ASJC Scopus subject areas

  • Food Science

Cite this

Effects of flavonoids on the expression of the pro-inflammatory response in human monocytes induced by ligation of the receptor for AGEs. / Huang, Shang Ming; Wu, Chi Hao; Yen, Gow Chin.

In: Molecular Nutrition and Food Research, Vol. 50, No. 12, 12.2006, p. 1129-1139.

Research output: Contribution to journalArticle

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