Effects of (-)-epigallocatechin-3-gallate on cyclooxygenase 2, PGE 2, and IL-8 expression induced by IL-1β in human synovial fibroblasts

Guo Shu Huang, Ching Ya Tseng, Chian-Her Lee, Sui Long Su, Herng Sheng Lee

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The objective of this study was to examine the effects of (-)-epigallocatechin-3-gallate (EGCG) on cyclooxygenase 2 (COX-2), prostaglandin E2 (PGE2), and interleukin 8 (IL-8) expression induced by IL-1β in human synovial fibroblasts. Cells were enzymatically isolated from synovial tissue taken from patients undergoing joint replacement surgery for osteoarthritis. Reverse transcriptase-polymerase chain reaction, immunocytochemistry, and western blotting were used to assess the COX-2 gene and protein expression with the associated mechanisms. PGE2 and IL-8 secretion into the culture medium was assayed by enzyme-linked immunosorbent assay. COX-2 upregulation in synovial fibroblasts induced by IL-1β was significantly suppressed by EGCG in a dose-dependent manner. PGE2 and IL-8 secretion was also induced by IL-1β stimulation and significantly suppressed by EGCG. The mechanism was associated with the phosphorylation of IKKβ. EGCG may inhibit the expression of inflammatory mediators, such as COX-2, PGE2, and IL-8, induced by IL-1β in human synovial fibroblasts. EGCG may be of value in the treatment of synovial inflammation.

Original languageEnglish
Pages (from-to)1197-1203
Number of pages7
JournalRheumatology International
Volume30
Issue number9
DOIs
Publication statusPublished - Jul 2010

Keywords

  • Cyclooxygenase 2
  • Epigallocatechin-3-gallate
  • IL-1β
  • IL-8
  • PGE
  • Synovial fibroblasts

ASJC Scopus subject areas

  • Rheumatology
  • Immunology
  • Immunology and Allergy
  • Medicine(all)

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