Effects of epidermal growth factor and linoleic acid on lipid contents in human intestinal C2BBE1 cells

Chiu Fang Liang, J. C J Chao, Shiaw M. Hwang, Ya H. Tsai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Epidermal growth factor (EGF) was reported to regulate triacyl glycerol synthesis in various cells. Linoleic acid and its metabolites were thought to modulate the signal transduction of growth factors. This study determined whether linoleic acid regulated the effect of EGF on lipid contents in human intestinal C2BBe1 cells. Confluent cells were incubated with serum-free medium (control), EGF (45 ng/mL), linoleic acid (42 μg/mL), or combined EGF (45 ng/mL) and linoleic acid (42 μg/mL) for 48 h. The results showed EGF and linoleic acid significantly increased intracellular cholesterol and triglyceride levels compared with the control and combined groups. EGF was a more potent stimulator for triacyl glycerol synthesis in C2BBe1 cells than linoleic acid. However, intracellular cholesterol and triglyceride levels did not differ between the control and combined groups. The secretion of cholesterol and triglyceride into the medium by C2BBe1 cells did not differ among four groups. Both EGF and linoleic acid strongly stimulated the expression of EGF receptor mRNA in C2BBe1 cells at 48 h compared with the control and combined groups. Therefore, EGF and linoleic acid increased triacyl glycerol synthesis in C2BBe1 cells through stimulating the expression of EGF receptor mRNA. The effect of EGF and linoleic acid on this lipogenesis was reversed in the presence of both EGF and linoleic acid by downregulating the expression of EGF receptor mRNA.

Original languageEnglish
Pages (from-to)275-279
Number of pages5
JournalIUBMB Life
Volume54
Issue number5
DOIs
Publication statusPublished - Nov 1 2002

Fingerprint

Linoleic Acid
Epidermal Growth Factor
Lipids
Epidermal Growth Factor Receptor
Glycerol
Triglycerides
Cholesterol
Control Groups
Messenger RNA
Signal transduction
Lipogenesis
Serum-Free Culture Media
Metabolites
Signal Transduction
Intercellular Signaling Peptides and Proteins
Down-Regulation

Keywords

  • EGF receptor
  • Epidermal growth factor
  • Human intestinal cells
  • Linoleic acid

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Effects of epidermal growth factor and linoleic acid on lipid contents in human intestinal C2BBE1 cells. / Liang, Chiu Fang; Chao, J. C J; Hwang, Shiaw M.; Tsai, Ya H.

In: IUBMB Life, Vol. 54, No. 5, 01.11.2002, p. 275-279.

Research output: Contribution to journalArticle

Liang, Chiu Fang ; Chao, J. C J ; Hwang, Shiaw M. ; Tsai, Ya H. / Effects of epidermal growth factor and linoleic acid on lipid contents in human intestinal C2BBE1 cells. In: IUBMB Life. 2002 ; Vol. 54, No. 5. pp. 275-279.
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