Effects of epidermal growth factor and its signal transduction inhibitors on apoptosis in human colorectal cancer cells

J. C J Chao, Wen L. Peng, Sheng-Hsuan Chen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aim: The study investigated if EGF signaling inhibitors, EGF antibody and tyrphostin 51 (a tyrosine kinase inhibitor), mediated the action of EGF on apoptosis and the expression of EGF receptors and p21 (a cyclin-dependent kinase inhibitor) of human colorectal cancer cells. Methods: Human colorectal adenocarcinoma cells (SW480) were incubated with 0.6 mL/L dimethyl sulfoxide (DMSO, the control group), 225 ng/mL (37.5 nmol/L) EGF in 0.6 mL/L DMSO, 225 ng/mL EGF+2.5 μg/mL (17 nmol/L) EGF antibody in 0.6 mL/L DMSO, or 225 ng/mL EGF+215 ng/mL (0.8 μmol/L) tyrphostin 51 in 0.6 mL/L DMSO. Results: After 48 h incubation, the levels of EGF in medium significantly increased (P

Original languageEnglish
Pages (from-to)540-544
Number of pages5
JournalWorld Journal of Gastroenterology
Volume10
Issue number4
Publication statusPublished - Feb 15 2004

ASJC Scopus subject areas

  • Gastroenterology

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