Effects of different arginine concentrations on angiogenic protein production induced by HeLa cells

Szu-Yuan Chou, Chun-Sen Hsu, Ming Yi Hsu, So Jung Liang, Chiu Li Yeh, Sung Ling Yeh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives: This in vitro study investigated the effects of different arginine (Arg) concentrations on angiogenic protein expressions of HeLa cells and endothelial cells (ECs) after stimulation. In addition, an inducible nitric oxide (iNO) synthase inhibitor (1400. W) was used to investigate the possible role of iNO in angiogenesis. Methods: Endothelial cells and HeLa cells were treated with different concentrations of Arg and 1400. W: Arg 0, 50, 100, and 1000 μmol/L; Arg 100 μmol/L. +. 1400. W 10 μmol/L; and Arg 1000 μmol/L. +. 1400. W 10 μmol/L for 24. h. Then, ECs and HeLa cells were cocultured for 2. h, and the supernatant in the transwell was collected for analysis of angiogenic protein secreted. The expression of CD51/CD61 by ECs was also analyzed. Results: The productions of vascular endothelial growth factor, basic fibroblast growth factor, prostaglandin E2, and matrix metalloproteinase-2 were higher with Arg 100 and 1000 μmol/L than with Arg 0 and 50 μmol/L Arg, and this was consistent with the expression of CD51/CD61 by ECs. Inhibition of iNO production resulted in lower angiogenic protein expressions comparable with groups with low Arg administration. Conclusion: The findings of this study suggest that Arg administration at levels similar to or higher than physiologic concentrations enhance the production of angiogenic protein and iNO may partly play a role in promoting angiogenesis in the presence of HeLa cells.

Original languageEnglish
Pages (from-to)818-822
Number of pages5
JournalNutrition
Volume26
Issue number7-8
DOIs
Publication statusPublished - Jul 2010

Fingerprint

Angiogenic Proteins
HeLa Cells
Arginine
Endothelial Cells
Nitric Oxide
Matrix Metalloproteinase 2
Nitric Oxide Synthase Type II
Fibroblast Growth Factor 2
Dinoprostone
Vascular Endothelial Growth Factor A

Keywords

  • Angiogenic protein
  • Arginine
  • CD51/CD61
  • Endothelial cells
  • HeLa cells
  • Inducible nitric oxide

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Effects of different arginine concentrations on angiogenic protein production induced by HeLa cells. / Chou, Szu-Yuan; Hsu, Chun-Sen; Hsu, Ming Yi; Liang, So Jung; Yeh, Chiu Li; Yeh, Sung Ling.

In: Nutrition, Vol. 26, No. 7-8, 07.2010, p. 818-822.

Research output: Contribution to journalArticle

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