Effects of dietary glutamine on inflammatory mediator gene expressions in rats with streptozotocin-induced diabetes

Pei Hsuan Tsai, Chui Li Yeh, Jun Jen Liu, Wan Chun Chiu, Sung Ling Yeh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objectives: This study investigated the effects of glutamine (Gln) supplementation on gene expressions of inflammatory mediators and cytokines associated with T-helper cell type 17 (Th17) regulation in diabetic rats. Methods: There were one normal control group and two diabetic groups in this study. Rats in the normal control group were fed a regular chow diet. One diabetic group (DM) was fed a common semipurified diet, and the other diabetic group received a diet in which part of the casein was replaced by Gln (DM-Gln), which provided 25% of the total amino acid nitrogen for 8 wk. Diabetes was induced by an intraperitoneal injection of nicotinamide followed by streptozotocin. Rats with blood glucose levels exceeding 200 mg/dL were considered diabetic. Blood samples and blood mononuclear cells of the animals were collected at the end of the study for further analysis. Results: Gene expressions of transforming growth factor-β1 and interleukin-17A did not differ in blood mononuclear cells among the three groups. Expressions of interleukin-6, interleukin-23, monocyte chemotactic protein-1, and the receptor of the advanced glycated endproducts gene were higher in blood mononuclear cells and the ratio of reduced to oxidized glutathione was lower in erythrocytes in the DM group than in the normal control group. Messenger RNA expressions of these genes were lower, whereas the ratio of reduced to oxidized glutathione was higher in the DM-Gln group than in the DM group. Conclusion: Supplemental dietary Gln increased the antioxidant potential and downregulated the expressions of inflammatory mediators. However, Th17 might not be an important involved pathway and the regulatory effect of Gln on Th17 immune response was not obvious in this animal model.

Original languageEnglish
Pages (from-to)288-293
Number of pages6
JournalNutrition
Volume28
Issue number3
DOIs
Publication statusPublished - Mar 2012

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Experimental Diabetes Mellitus
Glutamine
Gene Expression
Blood Cells
Glutathione Disulfide
Control Groups
Diabetic Diet
Diet
Interleukin-23
Th17 Cells
Niacinamide
Interleukin-17
Chemokine CCL2
Transforming Growth Factors
Streptozocin
Caseins
Intraperitoneal Injections
Blood Glucose
Interleukin-6
Nitrogen

Keywords

  • Diabetes
  • Glutamine
  • Receptor of advanced glycated endproducts
  • Reduced to oxidized glutathione ratio
  • Th17

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Effects of dietary glutamine on inflammatory mediator gene expressions in rats with streptozotocin-induced diabetes. / Tsai, Pei Hsuan; Yeh, Chui Li; Liu, Jun Jen; Chiu, Wan Chun; Yeh, Sung Ling.

In: Nutrition, Vol. 28, No. 3, 03.2012, p. 288-293.

Research output: Contribution to journalArticle

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