Effects of dextrose prolotherapy on contusion-induced muscle injuries in mice

Sen Wei Tsai, Yi Ju Hsu, Mon Chien Lee, Hao En Huang, Chi Chang Huang, Yu Tang Tung

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

© Ivyspring International Publisher. Current treatment options for muscle injuries remain suboptimal and often result in delayed/incomplete recovery of damaged muscles. In this study, the effects of dextrose prolotherapy on inflammation and regeneration of skeletal muscles after a contusion injury were investigated. Mice were separated into five groups, including a normal control (NC), post-injury with no treatment (mass-drop injury, MDI), post-injury with 10% dextrose (MDI + 10% dextrose), post-injury with 20% dextrose (MDI + 20% dextrose), and post-injury with 30% dextrose (MDI + 30% dextrose). The gastrocnemius muscles of the mice were subjected to an MDI, and muscle samples were collected at 7 days post-injury. Results showed the serum creatine kinase (CK), blood urea nitrogen (BUN), creatinine (CREA), and low-density lipoprotein (LDH) of the MDI-alone group were significantly higher than those of the normal control group (p<0.05). However, levels of serum CK, BUN, CREA, and lactate dehydrogenase (LDH) significantly decreased with different concentrations of dextrose. In addition, dextrose suppressed the macrophage response (F4/80 protein decreased) and promoted muscle satellite cell regeneration (desmin protein increased). In conclusion, dextrose prolotherapy can effectively help repair muscles; therefore, it may be one of the methods for clinically treating muscle injuries.
Original languageEnglish
Pages (from-to)1251-1259
Number of pages9
JournalInternational Journal of Medical Sciences
Volume15
Issue number11
DOIs
Publication statusPublished - Jul 30 2018

Fingerprint

Contusions
Glucose
Muscles
Wounds and Injuries
Blood Urea Nitrogen
Creatine Kinase
L-Lactate Dehydrogenase
Regeneration
Creatinine
Skeletal Muscle
Desmin
Serum
LDL Lipoproteins
Muscle Cells
Proteins
Macrophages

Keywords

  • Contusion
  • Dextrose
  • Diclofenac (DCF)
  • Mass-drop injury (MDI)
  • Prolotherapy

Cite this

Effects of dextrose prolotherapy on contusion-induced muscle injuries in mice. / Tsai, Sen Wei; Hsu, Yi Ju; Lee, Mon Chien; Huang, Hao En; Huang, Chi Chang; Tung, Yu Tang.

In: International Journal of Medical Sciences, Vol. 15, No. 11, 30.07.2018, p. 1251-1259.

Research output: Contribution to journalArticle

Tsai, Sen Wei ; Hsu, Yi Ju ; Lee, Mon Chien ; Huang, Hao En ; Huang, Chi Chang ; Tung, Yu Tang. / Effects of dextrose prolotherapy on contusion-induced muscle injuries in mice. In: International Journal of Medical Sciences. 2018 ; Vol. 15, No. 11. pp. 1251-1259.
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